This study aimed to analyze crude protein, amino acid and bioactive changes in Phellinus linteus HN00K9 cultured oat. The crude protein content of P. linteus cultured oat (PCO) was 12.9%, which was higher than that of uncultured oats (UCO) as control at 11.26%. The total free amino acid contents of PCO and UCO were 11,4 mg/100 g and 9,686.205 mg/100 g, respectively. Glutamic acid accounted for 21% of the total amino acids of PCO, and the histidine content was increased by more than 51% in PCO compared to UCO. The total polyphenol content of PCO was 275 mg GAE/g, which was more than twice that of UCO (135 mg). The DPPH radical scavenging activity was 15.5% in PCO, which was more than five times that of UCO (3.5%). The β-glucan content of PCO was 12.5 g/100 g, which was more than five times that of UCO (3.2 g/100 g). Therefore, it is believed that PCO can be utilized as a material for various functional foods.

This study aimed to evaluate the germination characteristics, -glucan and antioxidant contents, and antioxidant activity of naked oat according to germination temperature (20, 25, and 30oC) and time (24, 48, and 72 h). Sprout length was highest at 25oC, and the rotten degree was increased as the germination temperature and time increased. The -glucan content of naked oat increased after 24 h of germination at all temperatures and then decreased by 72 h of germination. Total polyphenol content, flavonoid content, and antioxidant activity increased as the germination temperature and time increased.

Recently, consumers’ awareness of the importance of the intestinal action of lactic acid bacteria and intestinal microbes is increasing, as well as interest in yogurt. In this study, yogurt was prepared with three mixed strains (lactic acid bacteria combination, Lactobacillus acidophilus, Lactobacillus delbrueckii subsp., and Bulgaricus, Streptococcus thermophilussei, 1:1:1) by adding oats flour, and the quality characteristics of yogurt were investigated, while stored at a storage temperature of 4℃ for 12 days. According to the storage period, the control as wel as the oat yogurt showed slight decrease in pH, and no significant change in acidity. Sugar content slightly increased. and brightness decreased, in the control and the oat yogurt. Visible cell numbers increased during storage, and decreased on the 12th day. Viscosity in the oat yogurt was 7,580 cP, which was approximately eight times higher than that of the control group, and decreased gradually according to the storage period. Antioxidant activity (DPPH) was approximately two times higher in the oat-added yogurt, and slightly increased with the storage period, decreased on the 12th day of storage, and β-glucan was detected only in oat-added yogurt.

In this study, we investigated the effects of adding oat and lactic acid bacteria on the quality and functionality of yogurt. Yogurt was fermented with various lactic acid bacteria,; Lactobacillus acidophilus (LA), Lactobacillus delbrueckii sub. bulgaricus (LB), and Streptococcus thermophilussei (ST) and quality properties, β-glucan content, antioxidant activity were estimated. The quality of control and oat added yogurt (OY) showed significant differences depending on the type of strain and combination. The addition of oats significantly accelerated the lactic acid bacteria production, decreased the pH, and increased the titratable acidity and count of the viable cells compared to the control. Acid production was highest in ST, with the complex strains containing ST and LALBST showing high quality characteristics. The viscosity of oat yogurt was higher than that of the control group, and LALBST was also significantly higher than that of the control group. The β-glucan content of OY was 0.14-0.2%, and the organic acid content and antioxidant activity were also significantly increased by the addition of oats. As a result, it is thought that the addition of oats and a combination of lactic acid bacteria can be used for improving the quality and functionality of yogurt.

The purpose of this study was to develop a functional senior mixed soymilk for seniors and to provide functional senior-mixed soymilk to prevent disease outbreaks. The isoflavone content of senior soymilk was 0.15±0.01 mg/g. The β-glucan content of senior soymilk was 9.82±0.01 mg/g. The weight gain of mice were significantly higher in the high-fat diet group than in the other groups. The serum triglyceride content was high at 102.67±40.61 mg/dL in the high-fat diet group, but significantly lower at 83.00±42.43 mg/dL in the 20% senior soymilk group. Thus, the results of this study comprehensively suggest that food intake should be ingested for each life cycle with reference to the dietary reference intakes for Koreans. In particular, the elderly in their 50s and older are deficient in protein and weakened immune capacity, so it is imperative that they maintain their health through various foods such as soymilk, which is evenly nutritious.

본 연구는 귀리의 겨, 겉껍질 및 잎을 에탄올로 추출한 후 항산화 및 항균활성을 조사하였으며, 추출물을 유화형 돈육 소시지에 적용하였을 때 품질 및 저장성에 미치는 영향을 알아보기 위하여 수행하였다. 귀리의 잎 추출물(OLE)의 총 페놀함량(145.99ug CAE/mg), ABTS 라디칼 소거 활성(35.07%) 및 FRAP 활성(32.77ug Fe2+/mg)은 다른 부위에 비해 유의적으로 높게 나타났다(p<0.05). 항균 활성 측정결과 모든 부위의 추출물에서 Staphylococcus aureus, Streptococcus pneumonia과 Escherichia coli에 대해서 활성을 보이지 않았으나, OLE 및 귀리 겨 추출물은 Klebsiella pneumonia에 대해서는 120μg/disk이상에서 활성을 보였으며, OLE가 귀리 겨 추출물보다 동일 농도에서 유의적으로 높은 활성을 나타내었다(p<0.05). OLE를 유화형 돈육 소시지에 첨가하였을 때, 0.025% 첨가구에서 pH, 조직감 및 관능적 특성에 유의적인 영향을 미치지 않았다. 하지만 저장 14일차에 모든 첨가구에서 지방산화(TBARS), volatile basic nitrogen (VBN), 총균수가 대조구보다 유의적으로 낮게 측정되었다(p<0.05). 본 실험결과 OLE는 높은 항산화 활성을 보였으며, 유화형 돈육 소시지 제품에 적용하였을 때 지방산화를 지연시켰고, 총균수의 저하를 가져왔기 때문에 천연 보존제로서 좋은 첨가제로 사료된다.

잡곡의 Fusarium 곰팡이독소의 오염 조사를 위해, 총 244개 잡곡시료(귀리, 수수, 율무, 기장)를 수확기 포장에서 2017년과 2018년에 수집하였다. 데옥시니발레놀(DON), 니발레놀(NIV), 제랄레논(ZEA)은 면역친화컬럼법과 UPLC를 이용하여 분석하였으며, 푸모니신(FUM)은 QuEChERS 방법과 LC-MS를 이용하여 분석하였다. 잡곡 시료 중 귀리의 NIV 오염수준은 120.0-3277.0 μg/kg로 다른 잡곡에 비해 가장 높았다. 율무에서는 DON이 최대 730.0 μg/kg 검출되었다. 기장의 NIV과 ZEA의 오염빈도는 각각 61.5% 와 57.9%로 높았으나 평균 오염량은 각각 75.6 μg/kg과 21.5 μg/kg로 안전한 수준이었다. 잡곡 시료 중 수수는 DON, ZEA, FUM의 오염빈도가 가장 높았으며, 2 종 이상의 Fusarium 독소 중복 오염률이 70.0%로 잡곡 평균 29.9%에 비해 높았다. 잡곡 재배포장에서 Fusarium 독소 오염을 안전하게 관리하기 위하여 독소 발생 모니터링과 함께 오염예방기술 개발 연구가 수행되어야 할 필요가 있다.

This study estimated the effect of sowing and harvesting dates on dry matter (DM) yield and feed value of forage oats at Sancheong, Korea. The forage oats (Darkhorse vs. Highspeed) were used in this experiment. The experimental main plots consisted of the different sowing and harvesting dates at 2 seasons as follows: spring oats of sowing (February 25, March 3 and March 13) and harvesting (May 27, June 6 and June 16); and fall oats of sowing (August 15, August 25 and September 4) and harvesting (October 15, October 25 and November 4). On spring oats, Highspeed sown on March 3 and then harvesting on June 6 had the highest (p<0.05) plant length and DM yield. Crude protein content decreased (p<0.05) in seed of the delayed-harvesting Highspeed. On fall oats, plant length and DM yield with the delayed-harvesting date increased (p<0.05), while crude protein content of the delayed-harvesting Highspeed decreased (p<0.05). This study concluded that the spring oat sown in early March and then harvesting in early June was recommended to increase dry matter and feed value although the fall oat sown in end August and then harvesting in early November was recommended for fall period.

고추장은 메주에서 자라는 세균, 곰팡이 등이 분비하는 분해효소의 작용으로 탄수화물을 당으로 분해시켜 단맛이, 단백질을 아미노산으로 분해하여 감칠맛이 증가된다. 담금 원료에 따라 저장 발효 중 생성되는 당과 아미노산의 종류와 양은 고추장의 품질에 영향을 미친다. 베타글루칸 함량이 높은 귀리 가공품의 다양화를 위해 볶은 귀리 분말을 20% 첨가하여 고추장을 제조하고 1개월 상온에서 발효 후 냉장(10℃), 상온(25℃)에서 5개월 총 6개월 동안 저장 발효하면서 이화학적 특성 변화와 관능을 조사하였다. 당도는 1개월 발효 후에 5.1°Bx였고 냉장, 상온 저장 4개월 후 가장 낮은 4.9°Bx, 4.4°Bx, 6개월에는 5.2°Bx, 5.4°Bx로 감소 후 다시 증가하였다. 수분 함량은 1개월 발효 후 44.0%에서 4개월째에는 냉장 저장은 44.1%, 상온 저장은 47.7%, 6개월째는 43.9%, 49.4%로 상온저장 발효 시 변화가 컸다. 총 유리아미노산 함량은 발효 1개월 후 3,936.1 μg/g에서, 3개월에 냉장 저장은 4,256.1 μg/g, 상온 저장은 4,300.0 μg/g로, 6개월 후에는 냉장 저장은 4,701.9 μg/g 상온 저장은 4,275.2 μg/g이었다. 베타글루칸 함량은 제조 직후 1.20%에서, 1개월 발효 후에 0.74%였고 상온 저장 3개월에 0.66%, 6개월에는 검출되지 않았고 냉장 저장 발효에서는 3개월에 0.74%, 6개월에 1.20%로 베타글루칸 함량이 유지되었다. 7점 척도법으로 관능평가한 결과 전체적 만족도는 발효 1개월 후 6.3에서, 냉장 저장 발효 3개월에 5.5, 6개월에 5.0으로 감소하였으며 상온 저장 발효는 3개월에 5.8, 6개월에 6.1이었다. 귀리분말 20%를 첨가한 고추장은 냉장 저장 발효 시에 당도, 수분, 총 유리아미노산, 베타글루칸의 함량 변화는 상온 발효 시 보다 적었으나 관능평가에서는 상온 발효 고추장의 평가가 높았다.

귀리는 미국 TIME지에서 기능성식품으로서의 가치를 인정하여 당뇨예방, 콜레스테롤 저하 및 심장병 위험 감소 효과를 명기할 수 있도록 허가된 10대 건강식품으로 소비자 관심과 수요가 증가하고 있으며 우리나라에서도 다양한 가공제품이 개발되고 있다. 선행 연구에서 베타글루칸 함량이 높은 조건으로 선발된 볶은 분말을 이용하여 첨가량별 귀리 고추장을 제조한 후 품질 특성을 조사하였다. 볶은 귀리 분말을 0, 5, 10, 15, 20%를 첨가하였다. 수분, 당도, pH, 총 산도는 처리에 따라 유의적인 차이가 없었으며 베타글루칸 함량은 귀리 첨가량이 0%에서 20%로 증가함에 따라 0.2%에서 1.2%로 유의적으로 증가하였다. 색도의 경우 볶은 귀리 분말 첨가량이 증가할수록 명도(L 값)는 15.1에서 16.9, 적색도(a 값)는 31.1에서 32.8, 황색도(b 값)는 26.1에서 28.8로 증가하였다. 총 유리당 함량은 볶은 귀리 분말 0% 첨가구(14.6%), 5%(14.6%), 10%(13.8%), 15%(12.7%), 20%(8.3%)로 첨가비율이 증가할수록 감소하였으며, 유리당 성분별 함량은 maltose(6.04∼4.92%) > glucose(5.69∼0.54%) > fructose(2.70∼1.91%) > sucrose(0.13∼0.94%) 순이었다. 총 유리아미노산 함량은 볶은 귀리 분말 0% 첨가구(221.4μg/g), 5%(2,775.5μg/g), 10%(2,715.5μg/g), 15%(2,805.1 μg/g), 20%(3,936.1 μg/g)로 첨가비율이 증가할수록 높아졌다. 필수아미노산 10 종의 총량과 glutamic acid 함량 역시 볶은 귀리 분말 20% 첨가 처리에서 가장 높은 161.5 μg/g, 1,066.9 μg/g이었다. 7점 척도법에 의한 관능조사 결과 대조구인 찹쌀고추장 대비 전반적인 기호도는 볶은 귀리 분말을 첨가한 고추장에서 높게 나타났으며 특히 볶은 귀리 분말 10% 첨가구에서 가장 높은 5.5이었다. 볶은 귀리 분말을 첨가한 고추장은 기호성과 영양면에서 우수하여 제품화가 가능할 것으로 생각된다.

'Dakyeong' (Avena sativa L.), a winter oats for forage use, was developed by the breeding team at National Institute of Crop Science, RDA in 2016. It was derived from a cross between ‘CI7505’(IT133304) and ‘Swan’(IT197920). Subsequent generations followed by the cross were handled in bulk and pedigree selection programs at Iksan and Jeonju, respectively. After preliminary and advanced yield test for 2 years, ‘SO2004009-B-B-10-8-3-9’, designated as a line name of ‘Gwiri91’, were subsequently evaluated for earliness and forage yield during 3 years in four parts such as Jeju (upland), Yesan (upland), Iksan (upland), and Jeonju (paddy), from 2014 to 2016, and finally named as ‘Dakyeong’. Cultivar ‘Dakyong’ has leaves of dark green color, thick diameter culm and long grain of brown color. Over 3 years, the heading date of ‘Dakyeong’ was about 5 days earlier than that of check cultivar ‘Samhan’ (April 30 and May 5, respectively), and their average forage dry matter yield harvested at milk-ripe stage was higher 12% (15.7 tone ha-1) than 14.0 tone ha-1 of check cultivar. Cultivar ‘Dakyeong’ was lower than the check cultivar ‘Samhan’ in terms of the protein content (6.1% and 7.0%, respectively) and total digestible nutrients (62.1%, and 62.5%, respectively), while the TDN yield was more than the check (7.79 tone ha-1 and 7.64 tone ha-1, respectively). Fall sowing cropping of ‘Dakyeong’ is recommended only in areas where average daily minimum mean temperatures in January are higher than -6°C, and it should not be cultivated in mountain areas, where frost damage is likely to occur.

Oats (Avena sativa L.), which are known as one of the forage crops of Korea, have good livestock palatability and are popular to cattle farmers because of their high dry matter. However, the cultivation of double cropping in the rice field was reluctant due to the late maturing for farmers to plant rice continuously. 'Hi-early', a winter oats for forage use, was developed by the breeding team at National Institute of Crop Science, RDA in 2016. It was derived from a cross between ‘517A2-121’(IT133383) and ‘CI7604’ (IT133379). Subsequent generations followed by the cross were handled in bulk and pedigree selection programs at Suwon, Iksan and Jeonju, respectively. After preliminary and advance yield test for 2 years, ‘SO2004015-B-B-23-1-3-7’, designated as a line name of ‘Gwiri92’, were subsequently evaluated for earliness and forage yield during 3 years in four parts such as Jeju (upland), Yesan (upland), Iksan (upland), and Jeonju (paddy), from 2014 to 2016, and finally named as ‘Hi-early’. Cultivar ‘Hi-early’ has the characteristics of medium leaves of green color, thick diameter culm, and medium grain of brown color. Over 3 years, the heading date of ‘Hi-early’ was about 9 days earlier than that of check cultivar ‘Samhan’ (April 26 and May 5, respectively). Average forage fresh yield of ‘Hi-early’ harvested at milk-ripe stage was similar to check cultivar (40.2 tone ha-1 and 40.0 tone ha-1, respectively), and dry matter yield also was similar to check cultivar (14.2 tone ha-1 and 14.0 tone ha-1, respectively). Cultivar ‘Hi-early’ was lower than the check cultivar ‘Samhan’ in terms of the protein content (6.2% and 7.0%, respectively) and total digestible nutrients (61.0%, and 62.5%, respectively), while the TDN yield was more than the check (7.91 tone ha-1 and 7.64 tone ha-1, respectively). Fall sowing cropping of ‘Hi-early’ is recommended only in areas where average daily minimum mean temperatures in January are higher than -6°C, and it should not be cultivated in mountain areas, where frost damage is likely to occur.

This study was conducted to investigate the feed value and silage quality according to storage period and film layers in whole crop oat silage. The crude protein content was increased in all silage during the storage periods compared to those before silage, under prolonged storage period slightly and the number of film layers of silage, six layer were higher than four layers, but no significant. NDF and ADF contents were also increased in all silage of storage duration compared to those before silage, but they was similar level between storage duration and number of film layers. TDN content was decreased of the storage duration. However, it was similar under the storage duration and the number of film layers. The pH value was decreased during prolonged storage period and six layers was lower than four layers depending on the film layers. In the organic acid contents, lactic acid and acetic acid were increased under the prolonged storage duration, and butyric acid was higher significantly(p<0.05), six layers of the film were showed higher lactic acid and lower butyric acid(p<0.05). Therefore, oat silage should be used within 6 months it was when treated with 4 layers, if considering the long-term storage, it is desirable to treat it with 6 layers or more.

This study examined the quality of bread made from a wheat flour, oat powder, and wild carrot powder mixture. The lightness and redness values decreased with increasing amount of wild carrot powder addition, while the yellowness was increased significantly. In a sample of wild carrot powder addition, the total volume was lower than the control. The texture profile analysis of oat bread, such as hardness, gumminess and cohesiveness decreased significantly with increasing amount of wild carrot powder addition. In the case of springiness, the value was not significant. The sensory evaluation of oat bread was significantly different in all analyses. Oat bread (BCB2.0) containing 2.0% (w/w) wild carrot powder showed the highest value in the four sensory evaluation items. Therefore, the 2.0% (w/w) addition of wild carrot powder addition was appropriate. An analysis of the physicochemical active component and DPPH scavenging activity of oat bread revealed a higher total flavonoid and total polyphenol content than normal bread. The DPPH scavenging activity was also 20.3% compared to the wild carrot powder. The availability of wild carrot powder in oat bread could be identified.

In the current study, we investigated the inhibitory activity of water soluble β-glucan from oat (Avena sativa) against various digestive enzymes such as α-glucosidase, sucrase, maltase and glucoamylase. Inhibition of these enzymes involved in the absorption of disaccharide can significantly decrease the post-prandial increase of blood glucose level after a mixed carbohydrate diet. The β-glucan had the highest documented rate of small intestinal sucrase inhibitory activity (2.83 mg/mL, IC50) relevant for potentially managing post-prandial hyperglycemia. Furthermore, we evaluated the effects of β-glucan on the level of post-prandial blood glucose in animal model. The post-prandial blood glucose levels were tested two hours after sucrose/starch administration, with and without β- glucan (100, and 500 mg/kg-body weight). The maximum blood glucose levels (Cmax) of β-glucan administration group were decreased by about 23% (from 219.06±27.82 to 190.44±13.18, p<0.05) and 10% (from 182.44±13.77 to 165.64±10.59, p<0.01) in starch and sucrose loading test, respectively, when compared to control in pharmacodynamics study. The β -Glucan administration significantly lowered the mean, maximum, and minimum level of post-prandial blood glucose at 30 min after meal. In view of the foregoing, it is felt that our findings suggest that β-glucan from oat serves to reduce post-prandial blood glucose rise secondary to slower absorption of glucose in the small intestine, via carbohydrate hydrolyzing enzymes inhibition.