From 2020, Korean Animal and Plant Quarantine Agency has reset the withdrawal time (WT) for veterinary drugs typically used in livestock in preparation for the introduction of positive list system (PLS) program in 2024. This study was conducted to reset the MRL for tiamulin (TML) in broiler chickens as a part of PLS program introduction. Forty-eight healthy Ross broiler chickens were orally administered with TML at the concentration of 25 g/L (TML-1, n=24) and 50 g/L (TML-2, n=24) for 5 days through drinking water, respectively. After the drug treatment, tissue samples were collected from six broiler chickens at 1, 2, 3 and 5 days, respectively. According to the previously established analysis method, residual TML concentrations in poultry tissues were determined using LC-MS/MS. In TML-1, TML in all tissues was detected less than LOQ at 2 days after drug treatment. In TML-2, TML in liver and kidney was detected more than LOQ at 2 days after treatment. According to the European Medicines Agency’s guideline on determination of withdrawal periods, withdrawal periods of TML-1 and TML-2 in poultry tissues were established to 0 and 2 days, respectively. In conclusion, the estimated WT of TML in poultry tissues is shorter than the current WT recommendation of 5 days for TML in broiler chickens.

The objective of this study was to investigate the anticancer effects of EMPS (edible mushroom mycelium polysaccharide: Tremella fuciformis) in animal models with colorectal cancer induced by AOM/DSS. The experimental groups consisted of Nor (normal), NC (AOM/DSS), EMPS (EMPS 50, EMPS 100), and PC (Fluorouracil). The NC group had the highest number of colon tumors, whereas it was observed that tumor occurrence was significantly reduced in the EMPS consumption group. The expression of Bcl-2, an apoptosis inhibitor, was significantly lower in the EMPS 50 & 100 and PC groups. On the other hand, the mRNA gene expression of Bax, a factor that induces apoptosis, was significantly higher in the EMPS 50 & 100 and PC groups compared to the NC group. The mRNA expression levels of TNF-α and COX-2 significantly increased in the NC group, but showed a significant decrease in the EMPS and PC groups, indicating inhibition of the cancer-promoting response of cells. At the phylum level of the mice's intestinal microbial composition, the proportion of Bacteroidetes tended to decrease, while the proportion of Firmicutes tended to increase with EMPS administration. This suggests that changes in the gut microbiota caused by inflammation can be influenced by dietary intake.

Because collagen is inherently piezoelectric, research is being actively conducted to utilize it to harvest energy. In this study, a collagen solution was prepared using edible low-molecular-weight peptide collagen powder, and collagen films were fabricated using a dip coating method. The collagen films prepared by dip coating showed a smooth surface without defects such as pinholes or cracks. Dehydrothermal treatment of the collagen films was performed to induce a stable molecular structure through cross-linking. The collagen film subjected to dehydrothermal treatment at 110 °C for 24 h showed a thickness reduction rate of 19 %. Analysis of the collagen films showed that the crystallinity of the collagen film improved by about 7.9 % after dehydrothermal treatment. A collagen film-based piezoelectric nanogenerator showed output characteristics of approximately 13.7 V and 1.4 μA in a pressure test of 120 N. The generator showed a maximum power density of about 2.91 mW/m2 and an output voltage of about 8~19 V during various human body movements such as finger tapping. The collagen film-based piezoelectric generator showed improved output performance with improved crystallinity and piezoelectricity after dehydrothermal treatment.

식용곤충은 미래식량 자원으로써 우수한 가치를 지니고 있어 해외에서는 사육자동화, IoT 및 AI 기술적용, 수직재배시스템 구축 등 많은 연구가 진행되고 있지만 국내에서는 대규모 사육농가나 곤충스마트팜 기술개발 이 부족하여 이를 위한 AI/빅데이터 인프라 구축이 시급한 실정이다. 학습용 인공지능 데이터는 식용곤충으로 활용되고 있는 장수풍뎅이, 흰점박이꽃무지, 갈색거저리, 백강잠, 메뚜기, 풀무치의 생애 주기별 총 6종의 RGB 사진데이터와 분광이미지 데이터 408,000장을 구축하였으며 온도, 습도, CO,, 암모니아, 조도, 수분 등 환경 데이 터 200,000세트를 수집하였다. 수집된 데이터는 원시데이터 수집, 원천데이터 가공, 라벨링 데이터 결합, 가공데 이터 검수 등을 통해 만들어졌으며 관련 데이터는 AI Hub(www.aihub.or.kr)에서 다운받을 수 있다. 확보된 식용곤 충 6종의 데이터는 곤충 종별 성장단계, 환경 변수에 따른 최적의 사육환경 조성, 생산시기 예측, 스마트대량사육 시스템 개발, 제품 가공시 추적이력제 도입, 식용곤충 스마트팜 기술 개발 및 연구 등 다양한 분야에 활용될 수 있을 것으로 예상된다.

Plastics are widely used in industries in human society and because of their structural stability, degradation is a serious global issue. To estimate the degradation of plastic, 31 edible mushrooms were cultured with the selected plastic films (polyethylene [PE], polystyrene [PS], and poly(ethylene terephthalate) [PET]) for 3 months at 25 °C. Measuring the weight of the films showed that four species of mushrooms, namely Porostereum spadiceum, Ganoderma lucidum, Coprinellus micaceus, and Pleurotus ostreatus, exhibited the highest degrees of plastic degradation. In addition, the mushrooms and fungi that exhibited the most significant plastic degradation were cross-cultured to promote this degradation. As a result, cross-cultivation of G. lucidum and Aspergillus niger showed a weight loss of 2.49% for the PET film. For the PS film, Aspergillus nidulans showed a weight loss of 4.06%. Cross-cultivation of A. nidulans and C. micaceus, which showed a weight loss of 2.95%, was noted as an alternative for PS biodegradation, but is harmful to humans. These bio-degradation effects of edible mushroom will contribute to the development of alternatives for eco-friendly plastic degradation.

This study was conducted to reset the maximum residue limit (MRL) for didecyldimethylammonium chloride (DDAC) in broiler chickens. The disinfectant containing DDAC (10%, w/w) was diluted 160 times and evenly sprayed on the bodies of twenty-four broiler chickens at a rate of 15 mL per day per bird for 7 days. After the disinfectant treatment, tissue samples were collected from six broiler chickens at 0.25, 1, 3 and 5 days, respectively. Residual DDAC concentrations in poultry tissues were determined using LC-MS/MS. Correlation coefficient (0.99 >), the limits quantification (LOQ, 2.0~10.0 μg/kg), recoveries (86.9~118.6%), and coefficient of variations (<19.98%) were satisfied the validation criteria of Korean Ministry of Food and Drug Safety. In all tissues except for liver, DDAC was detected more than LOQ at 5 days after the disinfectant treatment. In liver tissues, DDAC was detected more than LOQ at 3 days after treatment. According to the European Medicines Agency’s guideline on determination of withdrawal periods, withdrawal period of DDAC in poultry tissues was established to 26 days. In conclusion, the developed analytical method is sensitive and reliable for detecting DDAC in poultry tissues. When DDAC disinfectant is sprayed on a poultry house in the presence of broiler chickens, it is necessary to keep the disinfectant from contacting the body of the livestock.

This study was performed to investigate antioxidant and anti-inflammatory activities of perilla(Perilla frutescens L.) seed, flower and leaf according to extraction condition. Perilla seed extracts(PSE), perilla flower extracts(PFE), perilla leaf extracts(PLE) was extracted by stirring extraction (STE, 25°C), shaking extraction (SHE, 80°C), and sonication assisted extraction(SAE, , 25°C) with 94% ethanol, 60% ethanol and distilled water, followed by analysis of total polyphenol and flavonoid and testing radical scavenging activities. The highest total polyphenol content (5.47, 9.36, 38.58 mg gallic acid equivalent/g), total flavonoid content(5.77, 8.62, 46.44 mg catechin equivalent/g), ABTS(10.68, 19.46, 63.56 mg trolox equivalent/g) and DPPH(6.51, 7.69, 79.73 mg trolox equivalent/g) radical scavenging activity of PSE, PFE and PLE was observed in the HWE with 60% ethanol,. Among the three extraction method, SHE provided the best results for yield, polyphenol, flavonoid content of perilla seed, flower, leaf in comparison to STE or SAE. SHE with 60% ethanol of perilla seed, flower, leaf more effectively inhibited secretion of nitric oxide(NO) and pro-inflammatory cytokine in RAW 264.7 macrophage exposed to LPS compared to other extraction solvent and method. Therefore, these extracts obtained from perilla seed, flower, leaf could be used antioxidant and anti-inflammatory ingredients in the food industry.

This study investigated ethopabate (EPB) residues in edible tissues of broiler chickens given in drinking water and established the withdrawal time (WT) of EPB in poultry tissues. Twenty-four healthy Ross broiler chickens were orally administered with EPB at the concentration of 3.8 mg/L for 14 days (EPB-1, n=24) and 15.2 mg/L for 7 days (EPB-2, n=24) through drinking water, respectively. After the drug treatment, tissue samples were collected from six broiler chickens at 0, 1, 3, and 5 days, respectively. EPB residue concentrations in poultry tissues were determined using LC-MS/MS. Correlation coefficient values ranged from 0.9980 to 0.9998, and the limits of detection and quantification (LOQ) were 0.03~0.09 and 0.1~0.3 μg/kg, respectively. Mean recoveries in muscle, liver, kidney and skin/fat tissues were 95.9~109.8, 108.7~115.3, 89.9~96.6 and 86.7~96.8%, respectively, and coefficient of variations were less than 17.11%. At the end of the drug-administration period (0 day), EPB was detected at levels under the LOQ in all tissues from both the EPB-1 and EPB-2 groups. According to the results of EPB residue in Ross broiler tissues, withdrawal periods of both EPB-1 and EPB-2 in poultry tissues were established to 0 day. In conclusion, the developed analytical method is suitable for the detection of EPB in poultry tissues, and the estimated WT of EPB in poultry tissues will contribute to ensuring the safety of Ross broiler chickens.

From 2020, Korean Animal and Plant Quarantine Agency has reset the withdrawal time (WT) for veterinary drugs typically used in livestock in preparation for the introduction of positive list system (PLS) program in 2024. This study was conducted to reset the MRL for amprolium (APL) in broiler chickens as a part of PLS program introduction. Forty-eight healthy Ross broiler chickens were orally administered with APL at the concentration of 60 mg/L (APL-1, n=24) for 14 days and 240 mg/L (APL-2, n=24) for 7 days through drinking water, respectively. After the drug treatment, tissue samples were collected from six broiler chickens at 0, 1, 3 and 5 days, respectively. Residual APL concentrations in poultry tissues were determined using LC-MS/MS. Correlation coefficient (0.99 >), the limits quantification (LOQ, 0.3~5.0 μg/kg), recoveries (81.5~112.4%), and coefficient of variations (<15.5%) were satisfied the validation criteria of Korean Ministry of Food and Drug Safety. In APL-1, APL in all tissues except for kidney was detected less than LOQ at 3 days after drug treatment. In APL-2, APL in liver and kidney was detected more than LOQ at 5 days after treatment. According to the European Medicines Agency’s guideline on determination of withdrawal periods, withdrawal periods of APL-1 and APL-2 in poultry tissues were established to 3 and 2 days, respectively. In conclusion, the developed analytical method is sensitive and reliable for detecting APL in poultry tissues. The estimated WT of APL in poultry tissues is longer than the current WT recommendation of 2 days for APL in broiler chickens.

현재, 국내에서는 가공식품인 식용유지에 대한 잔류농약 허용기준이 설정되어 있지 않아 잔류농약은 식용유 품질평 가의 사각지대라 할 수 있다. 본 연구에서는 식용유지에서 가열증류법을 이용하여 68종의 농약을 대상으로 추출 및 정 제법을 최적화하여 GC-MS/MS 분석법을 확립하였다. 가열 증류법은 가열온도 및 시간의 영향을 받았으며 이동상의 역 할을 하는 질소의 유량과 용출용매의 종류에는 큰 영향을 받지 않는 것으로 나타났다. 본 연구에서 잔류농약의 결정 계수(R2)는 0.99 이상으로 나타났고, 정량한계(LOQ)는 0.01- 0.02 mg/L이었으며, 대두유를 이용하여 0.01, 0.02, 0.1, 0.5 mg/L 수준으로 회수율 실험 결과 평균 회수율(n=5)은 66.1- 120.0%이었고 상대표준편차는 ±10%이하로 나타났다. 또한 실험실내 일간정밀도는 11%이하로 조사되어, 식품의약품안 전평가원의 ‘식품등 시험법 마련 표준절차에 관한 가이드라 인(2016)’에 적합한 수준임을 확인하였다. 따라서 본 연구에 서 개발한 시험법은 식용유지의 잔류농약 안전관리를 위한 시험법으로 활용이 가능할 것으로 기대된다.

식용 옥수수 수확기는 수확 시 옥수수 이삭의 손상율을 결정하는 탈과 시스템이 중요하다. 탈과 시스템을 개발하기 위해서는 탈과 시스템의 손상율에 영향을 미치는 주요 설계 변수를 구명해야 하고, 설계 변수의 영향 분석에는 시뮬레이션 기법이 많이 사용되고 있다. 본 연구의 목적은 탈과 시스템의 시뮬레이션 해석에 사용할 옥수수 줄기에 대한 이산요소 모델을 개발하는 것이다. 식용 옥수수의 특성을 고려하기 위하여 옥수수 줄기의 물리적 특성을 분석하였으며, 만능재료시험기를 이용한 압축 시험과 3점 굽힘 시험을 통하여 역학적 특성을 확인하였다. 또한, 옥수수 줄기의 이산요소 모델은 접촉 모델과 결합 모델로 구현하였고, 매개변수 연구를 통해 이산요소 모델의 주요 파라미터를 도출하였으며, 시험 결과와 비교하여 개발된 이산요소 모델을 검증하였다. 본 연구로부터 개발된 옥수수 줄기 모델은 탈과 시스템의 시뮬레이션에 활용된다.

There has been increased interest in lignans due to their potential effect in reducing the risk of developing several diseases. To evaluate lignan contents, sensitive and accurate methods should be developed for their quantification in food. The present study aimed to validate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of 5 lignans: lariciresinol (Lar), matairesinol (Mat), pinoresinol (Pin), secoisolariciresinol (Seco), and syringaresinol (Syr). The validation included selectivity, linearity, recovery, accuracy, and precision. The method was proved to be specific, with a linear response (R2≥0.99). The limits of detection were 0.040~0.765 μg/100 g and the limits of quantification were 0.114~1.532 μg/100 g. Recoveries were 90.588~109.053% for black sesame powder. Relative standard deviations of repeatability and reproducibility were below 5%. Total lignan contents of roasted coffee bean, oat, and blacksoy bean were 105.702 μg/100 g, 78.965 μg/100 g, and 165.521 μg/100 g, respectively. These results showed that LC-MS/MS analysis would be effective in producing acceptable sensitivity, accuracy, and precision in five lignan analyses.