Background : Ginseng is perennial, frequently damaged by various diseases and insect pests, due to this, quantity and quality decreases. Particularly, Alternaria blight greatly damages the stem in its germination and the leaves after leafing. Here, this research conducted a test by investigating the control effect of lime-sulfur mixture in the market on 5 ginseng Alternaria blight, in order to utilize it as the disease control measure for disease and pest for eco-friendly cultivation of ginseng. Methods and Results : This research investigated the control effect of 5 types of lime-sulfur mixture in market on ginseng spotting disease by distributing the solvents to 5-years ginseng by 100 times and 200 times on April 29, May 15, and May 27. Also, this research investigated spore germination inhibition effect on Alternaria panax, Colletotrichum sp. and Botrytis cineria spore germination inhibition effect was investigated with spore germination inhibition ratio by manufacturing 100 times’ culture and 200 times’ culture per lime-sulfur mixture medium. There were two materials among 5 lime-sulfur mixture products to control over Alternaria blight using eco-friendly ginseng material, which didn’t damage during the leafing period of ginseng, and this research selected Sulfur 153, effective for Alternaria blight. Sulfur 153 was effective for Alternaria blight control due to its 82.2% control effect when treating by 200 times. Sulfur 153 was effective for spore germination inhibition of Alternaria panax, Colletotrichum sp. and Botrytis cineria and the rates of spore germination inhibition were 71.5, 100, 19.5% respectively. Conclusion : There were 2 materials among 5 lime-sulfur mixture products to control over Alternaria blight using eco-friendly ginseng material, which did not damage leafing period, and sulfur 153 was selected as the material effective for Alternaria blight. Sulfur 153 appeared to suppress germination of spore of Alternaria panax and Colletotrichum sp.

본고는 프란츠 리스트(1811-1886)의 즉흥연주에 대한 사고를 탐구한다. 1820-1850년대에 걸쳐 리스트는 서유럽 비르투오소 즉흥연주자로 그의 명성을 떨쳤고, 동시에 그의 헝가리-집시 즉흥연주에 대한 이해를 확충하며 그의 저서와 작곡에 반영하기도 하였다. 기존의 연구가 서유럽과 헝가리-집시라는 양대문화에 기인해서 리스트와 즉흥연주의 관계를 조명했다면, 본 논문은 그 두 문화의 융합과 연계에 초점을 맞춘다. 필자는 다음 네 가지에 특히 초점을 맞춘다. 첫째, 1820년대 즉흥연주에 대한 리스트의 문화융합적 사고에 주목한다. 둘째, 1830-1840년대의 즉흥연주에 대한 비판과 리스트의 해결책을 살펴본다. 셋째, 1840-1850년대의 즉흥연주 쇠퇴와 역으로 부상하는 리스트의 헝가리-집시 즉흥연주에 대한 탐구에 집중한다. 마지막으로, 이러한 흐름을 바탕으로 리스트에게 즉흥연주는 작곡과 연주의 경계를 모호하게 하는, 또한 서유럽과 헝가리-집시의 경계를 넘나드는 중요한 음악적 매개체였음을 이해한다. 이상의 네 가지에 주목하면서 필자는 리스트의 실용적이면서도 이성적인 대응, 평가절하적인 즉흥연주에 대한 진지한 탐구, 혁신적인 문화융합적 사고를 다각도로 분석한다.

글로우 고압 방전 방식에 의한 저온 플라즈마(Non-thermal Plasma, NTP) 공정은 액상에서 광전자, 전자, OH-라디칼, 전자기 에너지 등 반응성이 높은 화학물질의 형성할 수 있다. 이 NTP는 공기를 carrier 가스로 활용하여 반응성이 높은 화학종을 생성하는데, 유기물을 효과적으로 산화시키는 고도산화공법의 일종이다. NTP의 강력한 산화력에도 불구하고, NTP 공정에서 발현되는 오염물질의 분해 메커니즘과 그 중간부산물 및 최종 생성물의 경로는 명확하게 밝혀지지 않아 연구가 필요한 실정이다. 본 연구는 폐수 내 다양한 오염물질이 어떠한 메커니즘으로 분해되는가를 실험적으로 규명하고, 2종(sulfamethazine sodium salt와 sulfathiazole sodium salt)의 sulfonamide 계열 항생제에 NTP 공정을 적용하여 제거성능과 분해경로를 검증한다. 또한, 대상 항생제의 분해가 폐수처리 과정에서의 분해 동역학 및 생태 독성과 어떻게 관련되는지를 확인하기 위해 주요 중간부산물 및 신규생성물의 fate를 확인한다. NTP 공정의 진보된 효과를 증명하기 위해 반응성 화학종인 과산화수소 (H2O2)의 순간 생성량을 정량하였으며, OH-라디칼 생성의 간접지표인 N-Dimethyl-4-Nitrosoaniline (RNO) 물질의 분해동역학적 성질을 바탕으로 분해속도계수를 산출하였다. H2O2의 농도는 초기 NTP 공정 적용 시 300 mg/L 까지 증가하였고, 1440 min 적용 시 약 12 mg/L로 감소하며 OH-라디칼 생성과 오염물질 분해에 관여하는 것을 확인하였다. 또한 RNO 물질은 일차함수 형태로 감소하며, 0.91/hr의 속도로 제거되었다. LC-MS/MS (6410 LC-ESI/MS/MS (QQQ), Agilent, USA) 분석을 통해 검출된 중간부산물과 신규생성물을 통해 분해경로를 확인하였다. 문헌과 실험결과의 비교는 NTP 공정이 OH-라디칼 발생 및 산화 환원제와 반응종의 상호 작용으로 인한 산화력 측면에서 다른 산화 공정보다 우수함을 보여준다. Daphnia magna를 이용한 생태독성(Toxicity Unit, TU) 결과는 폐수처리에 대한 NTP 공정 적용이 반응성 화학종의 생성촉진을 통해 독성의 저감에 기여함을 보여주며, 실험결과 TU 값은 초기 2.2 대비 24 hr 적용 후 0.8로 크게 감소하였고, 충분한 체류 시간이 핵심 설계요소임을 밝혔다. 궁극적으로 본 연구는 항생제의 효과적인 제어 및 부산물 관리방안에 대한 유용한 정보를 제공한다.

Background : The fruit of Lycii fructus (Gugija) has beeen used as a tonic medicine and a long-term healthy food without side effect in Asia. An increase in the demand for natural healthy food, Gugija has been thought as a source of healthy foods, and then the extracts of dried Gugija have been frequently used as food ingredients. But, its extracts with Gugija alone has a slightly bad taste. Physiological characteristics of Gugija extracts were investigated by adding white ginseng (WG) and red ginseng (RG) to enhance taste. Methods and Results : Gugija extract were prepared by adding 0, 20, 40, 60, 80 and 100% of ginseng (WG and RG) to the weight of Gugija, respectively. Antioxidant activities of Gugija extracts were investigated DPPH and ABTS radical scavenging activity, SOD-like activity, FRAP, activity. As the amount of WG added increased, DPPH, ABTS radical scavenging activity, and FRAP activity of Gugija extract decreased. On the other hand, the physiological activity of Gugija extract increased with increasing amount of RG. The SOD-like activity of Gugija extract added up to 60% of WG and to 40% of RG was continuously lowered, in the addition amount thereafter, the activity increased as the addition amount increased. In components, As the amount of WG and RG added increased, the ginsenosides content of Gugija extract increased, the content of ginsenosides was higher in extract added RG than WG, but betaine and total flavonoid content decreased. Conclusion : From the above results, we may suggest that Gugija extracts by adding RG increased the antioxidant activities, SOD-like activity, and the preference degree.

Background : Korea ginseng root has been traditionally used as a tonic as it is stated to have the capacity to normalize body functions and strengthen systems that are caused by various stresses. But, white ginseng (WG) has lower antioxidant activity than other medicinal crops. Omija (Schisandra chinensis Baillon) has various physiological functionalities such as anti-cancer, anti-inflammatory, and antioxidant activities, which have the effective components of Omija are lignans (schizandrins and gomisins), and this components were contented mostly in seed part on Omija. Physiological characteristics of WG extracts were investigated by adding Omija to enhance functionality. Methods and Results : WG extract were prepared by adding 0, 20, 40, 60, 80 and 100% of Omija to the weight of WG. Physiological characteristics of WG extracts were investigated DPPH radical scavenging activity, FRAP, and nitrite scavenging activity. As the amount of Omija added increased, DPPH radical scavenging activity of WG extract increased proportionally. IC50 of 10 times water extracts were 0.455, 0.028 and 0.041 ml/ml on WG (100WG), WG added 50% Omija (50OM50WG), and Omija (100OM0WG), respectively. On the other hand, FRAP of extracts decreased slightly with increasing amount of Omija. Nitrite scavenging activity of Omija extract were significantly more active than WG extract, exhibited to 88.89% and 72.65% on WG extract added with 40% Omija (40OM60WG) and non added (100WG), respectively, pH 1.2. and then, to 33.45% and 1.38% with 40OM60WG and 100WG extract, respectively. Conclusion : From the above results, we may suggest that WG extracts by adding Omija increased the DPPH radical scavenging activity, nitrite scavenging activity and the preference degree on taste.

화석 연료 사용량 증가로 야기되는 지구 온난화를 비롯한 여러 환경문제와 자원 고갈로 인해 화석 연료를 대체 할 수 있는 연료원이 요구되고 있다. 이에 동식물성 기름으로부터 추출한 바이오디젤은 재생 가능한 연료로 주목을 받았으나 여러 한계를 보였고, 단위면적당 높은 에너지 수율과 빠른 성장속도 및 친환경적 특징을 겸비한 미세조류가 3세대 바이오디젤의 원료로 주목받고 있다. 본 연구는 독립영양과 혼합영양을 가능하게 하는 광 조건이 바이오매스 축적 지질의 지방산 특성 변화와 상관관계를 도출하여 바이오디젤 생산성 향상 방안을 고찰하였다. 본 실험에서 배양한 미세조류는 지질 축적능력이 우수한 Botryococcus braunii 종을 명암주기를 조절하여 광영양 및 혼합영양 조건으로 배양하였고, 회수한 바이오매스를 fatty acid methyl ester (FAME)형태로 지질을 추출하여 FAME 구성 성분과 FAME 수율을 비교 평가하였다. 실험결과는 독립영양과 혼합영양에 상관없이 C16, C18, C20 지방산이 주요 FAME의 구성 성분으로 밝혀져 바이오디젤로써 이용 가능성이 충분함을 확인하였다. 광영양 조건에서 전체 지질의 61.1%가 FAME 형태로 추출되었고 구성성분 중 C16, C18, C20 지방산의 함량이 각각 52.3%를 차지하였으며, FAME 수율(g FAME/g biomass)은 2.4%로 도출되었다. 혼합영양 조건에서 74.3%의 FAME이 추출되었고 C16, C18, C20 지방산의 함량은 73.9%를 차지하였으며, FAME yield는 3.1%로 도출되었다. 이 결과는 폐수로부터 수확한 미세조류 바이오매스가 대체 연료원으로 이용가치가 높음을 보여준다.

Background : The several studies on the characteristics of Korean ginseng cultivars and breeding lines have already been carried out the level of molecular classification analysis in Korea. In spite of where Geumsan is a representative place of Korean ginseng, Geumsan native species (breeding lines) have not yet been carry out analysis of morphological, genetic characteristics and relationship. We have plan to carry out morphological, genetic characteristics and relationship for Geumsan native species, breeding lines. Furthermore, We could be used diverse genetic resources for Ginseng breeding. Methods and Results : In this study, a total of 71 breeding lines and variety (GS97-1 - Geumwon) consisting of native ginseng collections from Geumsan was analyzed to identify for Korean ginseng variety respectively, and clustered for the selection of Geumsan native ginseng in Korea using DNA markers. We collected 71 Ginseng breeding lines from Geumsan. Analyses of the genetic characteristics of the collection were conducted for extraction gDNA using sprout. We were measured DNA concentration using QIAxpert (QIAGEN). Each DNA sample was quantified at the final DNA concentration of 5 ng/㎖ using sterilized distilled water. Korean ginseng 14 variety and 57 Ginseng breeding lines from Geumsan could be identified polymorphism using the selected 6 primer (MFGp183, MFGp130, MFGp110_E, UFGp163, MFGp108 and UFGp156). Conclusion : These finding could be used for morphological and genetic characteristics for produced native ginseng in Geumsan area. Futhermore, we could be used diverse genetic resources for Ginseng breeding.

Background : For ginseng breed development, mostly the pure-line separation method that registers excellent varieties after performing the regional adaptation test with the varieties selected through the preliminary and main productivity tests on selected excellent individuals cultivated in farms or excellent lineages for growing from collected lineages is used. Recently, the quality of ginseng is degrading while the yield decreases by 15 - 20% due to the high-temperature injury, the physiological disorder and/or the diseases caused by the climate warming so that development of disaster tolerant varieties strong against high temperatures, salts and diseases are demanded. For such reason, three lineages showing excellent overground and underground growing were selected from the gene resources collected from 1997 to 2000. And the productivity test has been conducted with these lineages having Chunpoong and Yunpoong, the existing varieties as the reference varieties. For the lineages excellent in growing, the variety registration will be performed after the regional adaptation test. Methods and Results : The results of productivity tests on the 3 lineages (GS98-3-4, GS00-58 and GS97-69) selected for excellent growing while having Chunpoong and Yunpoong as the reference varieties show that on the 2-years-old dormant roots, all of the selected 3 lineages are better in growing compared to the reference varieties. Especially, GS98-3-4 shows excellent leaf lengths and stem lengths while GS97-69 shows excellent leaf widths. On the overground growing of 3-years-old ones, GS00-58 and GS97-69 lineages are exceedingly more excellent compared to the reference varieties while the selected 3 lineages showing superb underground growing. Especially, GS98-3-4 lineage shows great root weights and diameters. Conclusion : According to the productivity tests on the 3 lineages selected for excellent growing, the selected lineages are excellent in both overground and underground growing compared to the reference varieties of Chunpoong and Yunpoong. On the 2-years-old ginsengs, GS98-3-4 shows excellent overground growing while GS97-69 shows excellent underground growing. On the 3-years-old ginsengs, GS00-58 and GS97-69 show excellent overground growing while GS98-3-4 shows excellent underground growing.

Background : The most problematic disease in Boxthorn is Anthracnose in open field. New variety with Anthracnose-resistant is good for high yield and Safe fruit production in open field. Therefore it is necessary to develop a new variety with Anthracnose -resistant and high yield. Methods and Results : The new boxthorn line, CB10511-104 was selected from the cross between CB09480-18 and Chunghong (IT258916) to breed new variety with high yield, Anthracnose-resistant and good color in 2011. Its preliminary yield test was performed from 2013 to 2014 and the selected line was named Cheongyang 26. Its regional yield trials were carried out in Cheongyang, Yesan and Jindo for 3 years, from 2015 to 2017. Cheongyang 26 was registered as the new variety, Cheonggang, in 2017. The specific characteristics were summarized as follows. Tree shape is semi-open type and the leaf is lanceolate and green color. The flowers are normal size and purple. The fruit is normal size, round oval and redish. The flowering was June 17 with medium flowering. Number of sprout branch by pruning was generated less than the check variety, Cheongmeong. The infection rates on leaves to Eriophys macrodonis Keifer of Chunggang, 54.4 percent, was higher than the check variety, Cheongmeong. Anthracnose on fruits in open field was slightly strong compared to the check variety, but check variety was Anthracnose-resistant. The content of betaine and free sugar in dried-fruits were higher than that of the check variety. The dried-fruit yield was increased about 26 percent in open field compared Cheongmeong. Conclusion : This variety ‘Cheonggang’ was suitable for cultivation in open field and rain-shelter plastic greenhouse because of Anthracnose-resistant and good color. It is necessary the companion variety because of self-incompatible. It’s suitable ‘Cheonggeum’ in open field, ‘Cheongun’ in rain-shelter plastic greenhouse.

Background : Anemarrhena asphodeloides Bunge is herbaceous perennial plant used for medicine with tuber root. This experiment was conducted to establish proper drying method of Anemarrhena asphodeloides for GAP post-harvest management manual. Methods and Results : Anemarrhena asphodeloides radix was cut in 3mm thickness after washing. Drying methods were air curing, sun drying, and hot-air drying (45℃, 50℃, 55℃, 60℃). Dry matter ratio (DMR) and moisture content (MC) were checked for every 12 hours for air curing and sun drying and checked for every 2 hours for hot-air drying. In 6 hours hot air drying at 45℃ and 50℃, DMR and MC were 39.9%, 38.4% and 13.5%, 12.8% respectively. In 4 hours hot air drying at 60℃, DMR and MC were 38.0% and 13.6% respectively. In air curing and sun drying for 4 days, DMR and MC were 37.0%, 37.6% and 12.3%, 13.1% respectively. Color value according to drying methods was higher as 74.57 in ℓ value under 50℃ hot air drying and lower as 65.35 under air-curing. a value was higher as 3.50 under air-curing and lower as 0.47 under 50℃ hot air drying. Conclusion : In 6 hours hot air drying at 50℃, MC was 12.8% and color value was higher as 74.57 in ℓ value and lower as 0.47and 22.59 in a value and b value respectively. This result showed that hot air drying for 6 hours at 50℃ was more proper for Anemarrhena asphodeloides radix drying.

Background : Cynanchum wilfordii is a perennial species of climbing vine belonging to the family Asclepiadaceae. Cynanchum wilfordii native to Korea has been cultivated and used for medicine with tuber root. Prevalent furrow size is 20 ㎝ in height for Cynanchum wilfordii cultivation in Korea. This experiment was carried out to find out yield and commercial characteristics according to furrow height in cultivation of Cynanchum wilfordii. Methods and Results : There were 4 furrow height of 10 ㎝, 20 ㎝, 30 ㎝, and 40 ㎝. Seedling transplanting was performed in early May with 40-day seedlings grown in pot from late March. Seedling establishment rates were similar as 96.7% - 99.7% in all treatments. Vine length, branch number, node number, stem diameter, leaf length, and leaf width were higher in furrow of 40 ㎝ and chlorophyll content was not significantly different according to furrow height. Main root number was more as 5.2 ea/plant in 20 ㎝ furrow and first lateral root number was more as 4.5 ea/plant but not significantly different in 20 ㎝ and 30 ㎝ furrow. Main root length was longer as 39.2 ㎝ and 37.0 ㎝ in 40 ㎝ and 30 ㎝ furrow height respectively. First lateral root length was longest as 25.0 ㎝ in 30 ㎝ furrow. Main root diameter was thicker as 24.9 ㎜ in 40cm furrow and thinner as 18.7 ㎜ in 10 ㎝ furrow. First lateral root diameter was thicker as 14.4 ㎜ in 20 ㎝ furrow and thinner as 11.2 ㎜ in 10 ㎝ furrow. Total yield and commercial yield in 40 ㎝ furrow were highest as 1,173 ㎏/10 a and 919 ㎏/10 a, 78.6% of total yield. Conclusion : It was considered that higher total yield and commercial yield in 40 ㎝ furrow was resulted from better aerial part growth including vine length, branch number, node number, stem diameter, leaf length, and leaf width and bigger main tuber root size.

Background : Cynanchum wilfordii is a perennial species of climbing vine belonging to the family Asclepiadaceae. Cynanchum wilfordii native to Korea has been cultivated and used for medicine with tuber root. Direct seeding and seedling transplanting and tuber root seed (below ∅10 ㎜) planting have been performed for cultivation. This experiment was carried out to investigate growth and yield characteristics of Cynanchum wilfordii according to planting method. Methods and Results : Direct-seeding and tuber root seed (TRS) planting were performed in early April and seedling transplanting with seedling grown for 40 days was performed in early may. TRS size was 2 – 3 ㎝ in length and 10 ㎜ and 20 ㎜ in diameter respectively. Seedling establishment rate was similar as 97.3% - 99.0% in all treatments. Vine length, branch number, node number and chlorophyll content were not significantly different in all treatments. Stem diameter, leaf length, and leaf width were higher in TRS planting. Main root number was more as 4.5 - 4.3 in seedling planting and TRS planting (∅20 ㎜) respectively but first lateral root number was not significantly different in all treatments. Root diameter was thicker as 23.3 ㎜ in TRS planting (∅20 ㎜). Total yield in TRS planting (∅20 ㎜) was higher as 1,041 ㎏/10 a, 25% increased compared to 830 ㎏/10 a of direct seeding and with higher commercial yield rate of 75.7%. Conclusion : It was considered that higher total yield and commercial yield in TRS planting (∅20 ㎜) was resulted from bigger size of stem diameter, leaf length, and leaf width and bigger main root size related to root yield and commercial value.

Background : Liriope platyphylla Wang et Tang is planting in April and harvest next April for the fourth season went through medicinal crops. This is why growth and impact on the quantity of additional fertilizer for application timing and amount of application is very important. To want to find out optimal method of additional fertilizer that can increase the quantity and quality application. Methods and Results : So this study was designed in order to find out proper timing for application and amount of applied fertilizer. Timing for application were July, September, November (custom) and May, July, September and August, October, December. Amount of applied fertilizer were 7 ㎏, 13 ㎏, 19 ㎏ per 10 a. The result of an experiment, when In cultivation with timing for application was May, July, September and amount of applied fertilizer was 7 ㎏ per 10 a, In situation of growth and development, plant length was short and root length was long and number of plants was many than timing for application was August, October, December and amount of applied fertilizer was 19 ㎏ per 10 a. In addition, in quantity characteristics, tuberous root number was 44 to 68 more than 24 and tuberous root length and tuberous root thickness was long and thicker. When these growth and development situation and the quantity characteristics, yield was 275 ㎏ to 340 ㎏ more than 65 ㎏ per 10 a. That is 5.2 times. Conclusion : In cultivation of Liriope platyphylla Wang et Tang, when the timing for application quickly start in May and reducing the amount of applied fertilizer was yield increased 5.2 times.

Background : Liriope platyphylla Wang et Tang are very sensitive to the excess moisture condition and dry crops. This is why the tuberous root is big and as many as the quantity for adequate water is needed. Study of proper bed width and hight to maintain optimal moisture for the high quality and yield. Methods and Results : So this study was designed in order to find out proper bed Width and hight. Bed width were 80 ㎝, 100 ㎝, 120 ㎝ and hight were 10 ㎝, 15 ㎝, 20 ㎝. The result of an experiment, when cultivation with bed width 100 ㎝, hight 20 ㎝, In situation of growth and development, plant length and root length were long and number was plants was many than bed width 120 ㎝, hight 10 ㎝. In addition, in quantity characteristics, tuberous root number was 27 to 58 more than 31 and tuberous root length and tuberous root thickness was long and thicker. When these growth and development situation and the quantity characteristics, yield was 66 ㎏ to 252 ㎏ more than 186 ㎏ per 10 a. Conclusion : In cultivation of Liriope platyphylla Wang et Tang, when to maintain optimal moisture with bed width 100 ㎝, hight 20 ㎝ have been higher quality and yield than cultivation with bed width 120 ㎝, hight 10 ㎝.

Background : Currently sprout ginsengs are produced in extensive variations of cultivation times, methods and specifications that are different in each farm in Korea. Also, regardless the effective ingredient content of ginseng, overgrown sprout ginseng are distributed so that the quality standardization of sprout ginseng is urgently required. The sprout ginseng market is only a segment of the Korean ginseng market still. However, If development of processed products utilizing the functionalities is connected, the market may exponentially grow. Also, if the use of sprout ginseng is extended to productions of drinks, cosmetics, facial masks and others in the cosmetics and health functional food industries, the export competitiveness to the global market is expected to increase. Therefore, this study aims the quality standardization of sprout ginseng to secure the market competitiveness in Korea and overseas by examining the property change following the cultivation phases. Methods and Results : For the overground growing in green houses, the sprout ginsengs planted in early April and early June show to be better in growing than the ones planted in early August. For the underground growing, while no significance is found among the cultivation periods, the sprout ginsengs planted in early June are excellent in terms of the root length and the weight of overground portion. Also, according to the examination on the stem properties of 2-year grown sprout ginsengs in each cultivation time, a trend that, as the growing period is longer from 25 days to 35 days, 45 days and 95 days, the hardness, gumminess, chewingness and springiness of stem are higher. And, on the hardness, gumminess, chewingness and springiness of leaf, while no statistically significance is found in the growing periods from 25 days to 45 days, the 95-day sprout ginsengs show high figures referring that the leaves becomes tougher. Conclusion : In case of cultivating sprout ginsengs in a green house, cultivating in April - June is advantageous for the overground and underground growing. And, it is determined that cultivating sprout ginsengs for 35 - 45 days produces leaves and stems with the most excellent chewing textures felt by consumers when chewing.

최근 제주지역에서의 폐기물관리문제가 가장 심각한 지역현안중 하나로 부각되고 있다. 제주지역 내에서 폐기물관리에서 부각되고 있는 대표적 사안들은 페기물 배출량의 급격한 증가, 빨라지는 매립장 만적현상 등으로 나타나고 있다. 사업장폐기물 발생의 지속적 증가로 인해 야기되는 폐기물 매립장의 조기 만적 문제를 해결하기 위해서는 효율적 폐기물 관리정책이 긴요하다고 판단되어 지는 시점이다. 본 연구에서는 제주특별자치도내에서 배출되는 폐기물의 배출 특성과 전국평균치와 제주도간 폐기물 발생현황을 비교한다. 또한, 두 행정시인 제주시와 서귀포시에서 배출되는 사업장폐기물을 중심으로 폐기물 배출량과 처리량에 대한 현황분석과 이를 통한 효율적 폐기물 관리방안에 대한 논의를 한다. 폐기물관리의 효율성은 공공부문의 노력과 민간부문과의 협업을 통해 높아질 수 있다. 공공부문측면에서 볼 때 해당 지자체 공무원들로 하여금 강력하고 효과적인 폐기물감축을 위해 폐기물 배출자에 대한 관리와 가이드라인 제시를 할 수 있는 실재적 권한을 부여해야 할 필요성이 있다. 민간부문과의 협업측면에서는 폐기물총량제의 일환인 폐기물 매립권 거래제(Landfill Trade)를 생각해 볼 수 있다. 다시 말해, 시장 기구를 통해 가격으로 하여금 일정수준이상을 넘어서는 매립의 초과수요를 해소 할 수 있도록 하여 폐기물매립에 대한 최적의 효율을 달성 할 수 있도록 하는 것이다.

인구의 증가와 산업화는 돼지, 소, 닭 등의 육류 식품의 급격한 수요증가를 초래하여 축산폐수발생량 역시 증가하였다. 2012년 해양투기의 법적 금지는 축산폐수 발생량을 173,304m3/day에 이르게 하였고 지속적인 증가추세에 있다(2016, 환경부). 축산폐수는 고농도의 질소, 인, 유기물을 포함하므로 수계 노출 시 부영양화를 유발하여 인간의 생활과 보건에 혼란과 악영향을 초래한다. 이러한 문제해결을 위해 혐기성 생물공학기술, 화학적 산화기술 등이 적용되어 왔으나 고부하에 취약하고 높은 비용을 요구하는 한계를 지닌다. 최근 새로운 대안으로 미세조류를 활용한 처리방법이 주목받고 있는데, 그 이유는 광독립영양 성장을 하는 미세조류의 특성상 빛에서 에너지를 얻어 경제적으로 지속가능하고 CO2를 탄소원으로 이용하여 탄소중립적으로 폐수 내의 고농도의 질소와 인을 동시에 처리할 수 있기 때문이다. 따라서 본 연구는 축산폐수내의 고농도의 유기물 및 영양염류를 동시에 가장 효과적으로 제거 가능한 미세조류를 문헌조사하여 대상 미세조류의 적용 타당성을 평가하였고, 최적 종을 선정하기 위한 성능평가 및 성장저해 분석을 수행하였다. 문헌조사를 통해 선정된 Scenedesums quadricauda, Scenedesums obliquus, Chlorella sorokiniana 을 28℃ 인큐베이터에서 500mL bottle의 용량으로 Phototrophic 조건(연속 빛조사)과 Mixotrophic 조건(16hr light-8hr dark 주기 반복)으로 Batch test를 진행하였고 그 결과를 성장 동역학적으로 해석하여 최적 종을 제시하였다. 실험은 BG-11을 대조구(Control)로 하여 미세조류의 cell counting결과를 바탕으로 비성장률(specific growth rate)을 도출한 결과 0.293 hr-1 (Scenedesums quadricauda), 0.302 hr-1(Scenedesums obliquus), 0.243 hr-1(Chlorella sorokiniana) 로 밝혀져 Scenedesums obliquus 가 가장 빠른 성장속도를 보였고, 축산폐수를 원수, 2배, 5배, 10배 희석을 하여 미세조류를 배양했을 때에도 최적의 유기물 및 영양염류 제거가 가능함을 보였다. 본 연구는 최적미세조류를 활용한 축산폐수 처리가 저에너지를 사용하며 기후변화에 대응하고 지속가능한 고농도 축산폐수처리 방법이 될 수 있음을 입증 하였다.

The purpose of this study was to confirm the applicability of aerobic granular sludge (AGS) in the advanced sewage treatment process. Simulated influent was used in the operation of a laboratory scale reactor. The operation time of one cycle was 4 h and the reactor was operated for six cycles per day. The volume exchange ratio was 50%. The influent was injected in divisions of 25% to increase the removal efficiency of nitrogen in every cycle. As a result, the removal efficiencies of CODCr and TN in this reactor were 98.2% and 76.7% respectively. During the operation period, the AGS/MLVSS concentration ratio increased from 70.0% to 86.7%, and the average SVI30 was 67 mL/g. The SNR and SDNR were 0.073 0.161 kg NH4 +-N/kg MLVSS/day and 0.071 0.196 kg NO3 --N/kg MLVSS/day respectively. These values were higher or similar to those reported in other studies. The operation time of the process using AGS is shorter than that of the conventional activated sludge process. Hence, this process can replace the activated sludge process.

Background: Gastrodia elata Blume is a saprophytic perennial plant in the Orchidaceae family, because of its agricultural and medicinal effectiveness, researchers focus on its genome and chemical components. However, cytogenetic information based on the chromosome structure and composition to construct chromosomal backbone for genome sequencing research and for the development and breeding of plants is very limited. Methods and Results: We determined the metaphase chromosome composition of the G. elata genome by fluorescence in situ hybridization (FISH) using 5S and 45S rDNAs and telomeric repeat probes. The nuclear genome of G. elata was organized into 2 n = 36, with relatively small (2.71 - 5.50㎛)chromosomes that showed gradual decrease in size. Conglutination phenomenon was observed among the metaphase chromosomes, and it was distinguished from that in other plant metaphase chromosome spreads. One pair of signal was detected for each 5S and 45S rDNA in the pericentromeric region and interstitial region on the short arm of chromosomes 10 and 4, respectively, and telomeric DNA signals were detected in the terminal region of most chromosomes. Conclusions: To our knowledge, this is the first FISH chromosome composition result in G. elata and could be useful in more comprehensive molecular cytogenetic and genomic analyses as well as breeding programs of the medicinal plant G. elata.

In the case of the port mooring facility, jetties with piles are mainly applied in soft ground conditions. In the three-dimensional modeling for the structural analysis of jetties with piles, the boundary conditions between the pile and the ground are universally applied to the Approximate depth of pile supported method and Elastic subgrade reaction spring method. In this study, p-y curve data for the pile ground condition was generated using the LPILE program, and then the nonlinear spring considering the p-y curve as the pile and ground boundary condition was input and analyzed in the 3D modeling of jetties with piles using the MIDAS civil And the necessity of applying the Nonlinear subgrade reaction spring method by comparison with the result and the analysis result according to the existing analysis method is suggested.