In this study, we found that the glycoprotein (18 kDa) isolated from Lactobacillus plantarum L67 (L67 protein) inhibits allergy-related inflammatory mediators in BPA treated rat basophilic leukemia 2H3 (RBL-2H3) cells and primary cultured splenocytes. First, we measured the levels of ERK, p38, and AP-1 by Western blotting and we evaluated the expression of IL-1β, IL-6, and IL-10 cytokines by real-time quantitative PCR. Finally, we evaluated the applicability of L. plantarum L67 as a yogurt starter culture by measuring pH, enumeration of bacteria, and sensory scores. The results of this study shows that L67 protein inhibits the phosphorylation of ERK and p38 MAPK, as well as the activation of AP-1 (c-Fos and c-Jun). With inflammationrelated factors (Th2-related cytokines), L67 protein inhibits BPA-induced IL-1β, IL-6, and IL-10 activity. Bisphenol A stimulates MAPK signaling, which induces increased GATA-3 expression, and GATA-3 induces the differentiation of Th2 cells and secretion of Th2-related cytokines. Our findings showed that L67 protein may regulate the activation of GATA3 and T-bet through the induction of MAPK signaling, and it relieves the Th1/Th2 cytokine profile imbalance. Taken together, these findings demonstrate that the L. plantarum L67 strain is a potential food supplement and starter culture, that could help in the prevention of Th2-related immune disorders.

Irrigation with saline water of rivers and groundwater reservoir into paddy field cause decreases rice production depending on water salinity. The purpose of this research was to determine the critical concentration of water salinity for provision of a basic information on the stable rice production in west costal area of Honam region. A greenhouse experiment was conducted at the 13 levels of saline water [0.01(control) 0.03, 0.05, 0.08, 0.10, 0.13, 0.15, 0.20, 0.25, 0.30, 0.50, 0.70, 1.00%] in transplanted rice using c.v. Cheongho and tolerance to different water salinities was evaluated at three growth stages of the rice plant such as [(35 DAT:Tillering), (81 DAT:Heading), and (106 DAT:Ripening)], respectively. Increasing water salinity significantly decreased grain yield and the higher in salinity the severe effect on yield reduction. The growth responses to water salinity varied at different growth stages of rice. At 35 DAT, increased water salinity decreased plant height and number of tillers significantly. Higher water salinity delayed the days to heading by 2 days (0.05%), 4 days (0.08%) and 7 days (0.1%). The critical water salinity at 35 DAT was below 0.08%. At 81 DAT, Cheongho survived at 0.10 and 0.08% salinity, respectively. However, at water salinity levels above 0.10%, the yield components such as number of panicles and number of spikelet decreased drastically. Thus, it was suggested that the critical water salinity at the mid-stage (tillering~heading, 81 DAT) was 0.05%, At 106 DAT, based on yield index that gives above 50% grain yield, the 0.05% salinity showed the lowest 61.1% grain yield of 19.2 g/plant as compared with the highest grain yield of 31.4 g/plant at 0.01% (control).

We developed a new ecotoxicological test method using native test species, eggs of Ephemera orientalis, and five kinds of industrial wastewater were tested to validate the test method. The water samples were collected in Jun 2006 from the following industries: pesticide, metal plating, PCB, leather1, and leather2. Wastewater and effluent were diluted by distilled water, respectively, to prepare various concentrations, 100, 50, 25, 12.5, 6.3, 3.1, and 0%. For the egg bioassay, 20 freshly laid eggs (<24 h old) were exposed to test solutions in a Petri dish (52×12 mm) at 20℃ with photoperiod of 16 h light and 8 h dark for 14 days. The median egg hatching concentrations (EHC50) were estimated using Probit analysis. All EHC50s of wastewater were less than 3.1%, which meant very high ecotoxicity except for the wastewater of PCB industry having 6.1% of EHC50. Among the effluents, the least toxic effluent was from pesticide industry having 58% of EHC50, while the effluent of leather2 was the most toxic having 7.3% of EHC50.

Two types of connection between the abutment and CFT pile were proposed for the sub-structure of the integral abutment bridge. One was a bolt connection (Type-A) and the other was a hook connection (Type-B). To analyze the performance of connection Type-A and Type-B, a quasi-static experiment was carried out. According to experimental results, the destruction occurred at the connection point and so both type A and type B need to have a reinforced design as the link between the abutment and CFT pile. However, when the load resistance performance and energy dissipation capacity was analyzed, the performance of connection Type-B was superior to the performance of connection Type-A.

Recently, in Korea various kinds of genetically modified (GM) crops have been imported and used as a raw material to manufacture foods and livestock feeds, but the different social concerns about the benefits and the potential risks of GM crops are being shown with a different reaction from the public. Thus a persistent management is required for the safe utilization of genetically modified organism (GMO). PCR analysis of transgene into crop is generally performed for the efficient post management of GMOs. The most important prerequisite for the application of nucleic acid detections is to decide the effective DNA-extraction methods. Particularly, in the case of processed feeds, the nucleic acids of which may be damaged by heating, high pressure, pH treatments, fermentation, etc. in processing, DNA must be extracted with high sensitivity from the samples to perform the PCR successfully. In this study, seven of DNA-extraction methods used commercially and non-commercially were compared with respect to the yields and quality of DNA extracted from livestock feeds and those crop materials. Amounts of genomic DNA obtained from the extraction methods varies according to feed configurations and crop materials. The DNA yield and uniformity of samples extracted with PG, CTAB, and QF method is greater than that obtained from other extraction methods. In the DNA integrity of the selected extraction methods, PCR analysis showed distinct amplifications and similar patterns in detecting crop endogenous genes and GMO genes. These results would be applicable for the selection of an adequate DNA-extraction method in extracting processed feeds and/or crop materials.

본 연구에서는 건축폐기물로 분류되는 레미콘회수수를 재활용함과 동시에 지구온난화의 주범 인 CO2의 자원화를 위한 시스템의 공정최적화를 진행하였다. 레미콘회수수를 이용한 액상탄산화 반응에 서 가장 중요한 공정은 Ca2+를 용출하는 공정이다. 일정량의 레미콘회수수를 이용해 고순도의 CaCO3을 생성하기 위해 Ca2+ 용출시 질산에 의해 낮아지는 pH 농도를 기준으로 실험을 진행하였으며, CO2는 발전기 배기가스를 이용해 MEA용액에 포집하였다. 본 연구를 통해 1톤의 레미콘회수수에서 최대 11 kg의 CaCO3를 합성할 수 있었다. 생성된 CaCO3 분석결과 제지용으로 사용 가능한 것을 확인하였다.

본 연구에서는 액-액 반응에 의한 액상탄산화법을 이용하여 탄산칼슘을 제조하였다. MEA를 사용하여 습식화학수법의 셔틀메카니즘을 도입하였다. MEA 30% 수용액에 고농도 이산화탄소(A)와 배 기가스(B)를 사용하여 이산화탄소를 포집하였으며, 액상탄산화과정을 통해 슬러지 mg 당 0.35 mg의 이 산화탄소를 고정하였다. 최종생성물의 SEM 분석결과 탄산칼슘의 구조는 calcite가 혼합되어 있으나 대 부분 구형 vaterite가 생성되었다.

본 연구에서는 고체추진기관의 2차원 축대칭 FSI 해석에서 CSS 및 ISS 기법의 수치 안정성을 고찰하였다. 이를 위하여 CSS 및 ISS 기법을 2차원 축대칭 FSI 수치해석 알고리즘에 구현한 프로그램을 작성하고, 이를 ACM 및 BCM 고체추진기관의 복합거동 해석에 사용하였다. 해석 결과들을 비교 분석하여 ISS 기법이 고체추진기관 FSI 해석의 수치 안정성 개선에 효과적인지 검토하였다. 연구결과, ISS 기법을 적용한 FSI 해석은 시스템 시간간격이 작아질수록 수치적 수렴성을 보이며, CSS 기법과 다르게 시간이 진행되어도 수치해의 진동이 발산하지 않는 것을 확인할 수 있었다. 이를 통하여 ISS 기법을 사용하면 ACM 및 BCM의 FSI 해석에 CSS 기법을 이용할 시 나타나는 수치 불안정성을 개선할 수 있음을 확인하였다.

본 조사를 통하여 2015년 6월부터 8월까지 태안군에서 13개 지역을 선정하여 곤충상을 조사 하였다. 주요조사방법으로 임도와 등산로, 생태탐방로를 따라 포충망을 이용한 채어잡기와 쓸어잡기 방법으로 주간 채집을 실시하였고, UV light trap을 이용한 야간 채집을 수행하였다. 채집된 육상곤충은 총 12목 77과 491종 3657개체가 확인되었고, 이 중 고유종으로 줄우단풍뎅이 (Gastroserica herzi Heyden, 1887, 딱정벌레목 : 검정풍뎅이과), 우리벼밤나방 (Sesamia pseudoturpis Kononenko et Ahn, 1998, 나비목 : 밤나방과), 제일줄나비 (Limenitis helmanni Lederer, 1853, 나비목 : 네발나비과), 팔공산밑들이메뚜기 (Anapodisma beybienkoi Rentz et Miller, 1971, 메뚜기목 : 메뚜기과)와 기후변화생물지표종 먹그림나비 (Dichorragia nesimachus Boisduval, 1836, 나비목 : 네발나비과), 암끝검은표범나비 (Argyreus hyperbius Linnaeus, 1763 나비목 : 네발나비과), 제주등줄박 각시 (Marumba spectabilis Okamoto, 1924, 나비목 : 박각시과)가 확인되었다. Shannon’s diversity 지수(H)와 Simpon’s diversity 지수(D′)를 이용하여 각 지역별 다양성을 산출하였는데 안면도 휴양림 (H=4.486, D′=0.9804)이 가장 높게 나타났으며, 대소산 (H=4.175, D′=0.9622)이 다음으로 높은 다양성을 보였다. 주성분분석(Principal Component Analysis) 결과 월별 종 구성의 차이는 크게 나타나지 않았지만, 지역별 구성에서 백화산, 안면도 휴양림, 대소산에서 다른 군집과 매우 상이한 종 구성을 보였다.

The concentration of VOCs, NO2 was measured both inside and outside residential homes surrounding an industrial complex. Measurements were performed in the area of the industrial complexes and around 10 km away from the industrial complex area. Benzene did not exceed the air quality standard value. Toluene exhibited a high value of concentration in outdoor Yeosu investigated group. The concentration of NO2 is higher than outside concentrations of houses in both inside housing research group compared with the group of Gwangyang and Yeosu. Benzene and toluene showed high correlation (p<0.001) in the housing interior in Gwangyang, It showed a high correlation (p<0.01) in the housing interior in the comparison group. In Yeosu there was a high correlation (p<0.001) between the inside and outside of the housing in the survey group. In the control group there was only high correlation (p<0.05) in the inside of the housing.

This study was conducted, using 10 high-quality rice varieties, at 24 experimental sites in 2013 and 2014 to analyze the comparative relationship among head rice yield (HRY), its attributes and sub-attributes. In the data set pooled across sites, years and varieties, where regional and varietal variations were combined, HRY was associated with milled rice yield (MRY) more closely than percentage of head rice (PHR) although both MRY and HRY demonstrated significant relationship with HRY. In the PHR higher than 80%, compared to 80% or less, fitness of the regression between PHR and HRY decreased. MRY and PHR, the two attributes of HRY, were not significantly associated with each other. Regional variation of HRY was associated only with MRY while varietal variation of HRY was related to both MRY and PHR. HRY and MRY showed regional variation greater than varietal variation meanwhile PHR demonstrated regional and varietal variations in a similar range. In the data set pooled across sites, years and varieties, MRY demonstrated relationship with spikelets per square meter (SPIK) in a higher significance than percentage of grain filling (PGF) and 1000-brown rice weight (TBRW). Both regional and varietal variations of MRY showed significant relationship only with SPIK, neitherwith PGF nor TBRW. It is concluded that regional variation of HRY was affected by MRY, varietal variation by both MRY and PHR, and region-variety combined variation mainly by MRY.

방역지리정보시스템이란 지리정보(Geographical Information)의 정밀 위치 및 환경 정보를 이용하여, 감염병 매개체를 감시, 방제, 관리하기 위해 전산체계로 구성된 감염병 매개체 방제관리 시스템으로 매개체의 발생정보를 근거로 한 과학적이고 효율적인 방제를 실현하기 위해 개발되었다. 감염병 매개체를 방제관리하기 위해서는 전문성과 축적된 정보를 활용해야 하나 방역을 전담하는 보건소의 방역 담당자의 잦은 교체로 인한 보건소의 방역업무의 전문성 부재 및 방역인력 부족으로 인한 업무 관리의 어려움이 대두 되며, 관리 업무에 필수적인 매개체 발생 취약환경 정보 부재, 방역업무에서 생산되는 중요자료 DB 소멸로 인해 감염병 매개체에 대한 방제관리가 미흡하였다. 이에 질병관리본부 질병매개곤충과는 ‘2006년 보건복지부 건강증진기금사업 정책과제’ 채택으로 2006년에서 2007년까지 전국 5개 시·군 보건소를 대상으로 시범 구축을 시행 하였고 2012년 기존 C/S 환경의 프로그램을 Web 환경의 프로그램으로 고도화 하여 현재 16개 시·군 보건소에 보급 및 운영 하고 있다. 또한 전국 확산 및 보급을 위해 준비 중이며 향후 기후데이터(빅데이터)를 활용한 예측관리의 진화 및 다양한 정보를 제공 할 수 있는 종합 방제관리 시스템 구축을 목표로 하고 있다.