In this study, we propose a data-driven analytical framework for systematically analyzing the driving patterns of autonomous buses and quantitatively identifying risky driving behaviors at the road-segment level using operational data from real roads. The analysis was based on Basic Safety Message (BSM) data collected over 125 days from two Panta-G autonomous buses operating in the Pangyo Autonomous Driving Testbed. Key driving indicators included speed, acceleration, yaw rate, and elevation, which were mapped onto high-definition (HD) road maps. A hybrid clustering method combining self-organizing map (SOM) and k-means++ was applied, which resulted in eight distinct driving pattern clusters. Among these, four clusters exhibited characteristics associated with risky driving such as sudden acceleration, deceleration, and abrupt steering, and were spatially visualized using digital maps. These visualizations offer practical insights for real-time monitoring and localized risk assessment in autonomous vehicle operations. The proposed framework provides empirical evidence for evaluating the operational safety and reliability of autonomous buses based on repeated behavioral patterns. Its adaptability to diverse urban environments highlights its utility for intelligent traffic control systems and future mobility policy planning.

The 2024 Noto Peninsula Earthquake in Japan caused significant damage to wooden structures due to strong ground motion and widespread liquefaction. This study examines the damage patterns observed in wooden houses and analyzes the underlying structural vulnerabilities that contribute to these patterns. Key findings include standard failure modes such as foundation settlement, soft-story collapse, torsional deformation, and joint failure. The evolution of Japan’s seismic design standards has been reviewed, highlighting the importance of balanced wall distribution, reinforced connections, and lightweight roofing systems. Based on field investigations, this study systematically classifies the types of seismic damage in wood structures. It develops a qualitative performance curve that visually illustrates structural behavior in terms of inclination, seismic damage patterns, and repairability. Furthermore, a seismic damage taxonomy is proposed to infer the causes of damage through logical links between observed failure patterns and structural vulnerabilities. This research establishes a practical foundation for the seismic design and retrofit strategies of wood structures, ultimately contributing to the advancement of earthquake-resilient construction practices.

This study was conducted to analyze odor regulation policies of major countries and explore development directions for Korea’s odor management system through international comparison. Korea has achieved significant progress in odor management over the past 20 years since the enactment of the Odor Prevention Act in 2004, including the establishment of real-time monitoring systems, integrated complaint management systems, and scientific management infrastructure in major odor management areas such as Sihwa-Banwol Industrial Complex and metropolitan landfills in Incheon and Daejeon. To identify potential development directions, a literature review and comparative analysis were conducted on four regions: the United States, the European Union, Japan, and Singapore. The analysis revealed that each region has developed unique approaches: Japan’s Odor Judge system (involving 3,352 active professionals) for measurement standardization; the United States’s citizen science-based monitoring systems, such as the Odor Explore project that utilizes community participation; the EU’s preventive management policies through the application of Best Available Techniques (BAT) and land use planning integration; and Singapore’s systematic management approach through its myENV app and urban planning integration. Based on Korea’s existing achievements and international experiences, this study identified eight development areas, including measurement system standardization, management scope expansion, integrated management system enhancement, real-time monitoring implementation, technology development investment, complaint resolution mechanism improvement, preventive management system enhancement, and information transparency enhancement. This study proposes development directions that build upon Korea’s current infrastructure and policy achievements, considering the country’s unique socio-economic conditions and environmental characteristics. These directions are expected to contribute to the continuous advancement of Korea’s odor management system while leveraging existing strengths and addressing future challenges.

This study analyzed policy measures to comprehensively achieve two goals: carbon neutrality in buildings and optimization of indoor air quality. While buildings account for approximately 40% of total energy consumption and greenhouse gas emissions, and present-day individuals spend 90% of their daily lives indoors, both goals are critically important. However, these objectives often conflict with each other, and current policies have limitations in effectively addressing this complex relationship. Analysis of related policies, including the Green Building Creation Support Act and the Indoor Air Quality Management Act, revealed significant drawbacks such as the lack of an integrated approach due to policy fragmentation, insufficient consideration of lifecycle carbon emissions, imbalance in economic incentive structures, and rigidity in technical standards. To overcome these challenges, this study proposes innovative improvement measures, including the following: establishing an integrated policy framework, introducing a multi-layered air quality management system, expanding performance-based design that simultaneously considers energy efficiency and indoor air quality, developing region-specific policies, implementing AI-based self-assessment systems, mandating green space ratios, controlling high-radon concentration areas, and expanding government incentives.

CRISPR/Cas9 ribonucleoprotein (RNP)-mediated gene editing has recently been applied to Ganoderma lucidum as a promising tool for functional genomics and strain improvement. However, the multinuclear nature of this basidiomycete can result in genetic mosaicism, raising concerns about the long-term stability of edited strains. In this study, we report the occurrence of revertant phenotypes in CRISPR/Cas9-edited transformants of G. lucidum. Although the edited colonies initially exhibited the expected phenotype, repeated subculturing led to the reappearance of wild-type phenotypes. PCR and sequencing analyses revealed the coexistence of edited and non-edited nuclei, and the progressive loss of edited genotypes over successive generations. These findings demonstrate that multinuclearity is a key factor contributing to the instability of CRISPR/Cas9-based edits in G. lucidum. This brief report provides the first direct documentation of revertant occurrence in edited G. lucidum strains and underscores the need for rigorous selection strategies and novel approaches to secure stable homokaryotic transformants in mushroom genetic engineering.

This study reports the development of a new pure white Flammulina velutipes variety, ‘Seoldan’. The breeding process involved a primary cross between a brown strain (KMCC02267) collected by the Rural Development Administration and a white cultivar ‘Baekwoon’ (KMCC05472). From this cross, a white line ‘Fv-23-553’ was selected and subsequently crossed with a superior pure white resource (KMCC05474), resulting in the novel cultivar Seoldan. Mycelial growth tests showed that both varieties grew best at 25°C; however, Seoldan exhibited faster mycelial growth than the control variety under four temperature conditions except at 20°C. In media tests, Seoldan also demonstrated superior growth on three media types, with the exception of YM medium. In sawdust bottle cultivation, The time from scratching to the first fruiting was 2 days shorter in Seoldan than in the control variety, contributing to an overall reduction in cultivation time. The most distinct morphological trait of Seoldan is its pure white cap color. The average yield was 218.8 ± 10.6 g per bottle, comparable to that of the control. Morphologically, Seoldan produced smaller pileus and thinner stipes than the control. Furthermore, somatic incompatibility tests confirmed that Seoldan is genetically distinct from the control variety. These results indicate that Seoldan is a promising pure white variety with stable productivity, improved cultivation efficiency, and clear genetic differentiation from existing varieties.

This study analyzed the structural performance of a microalgae-based lightweight ecological integration system for large-span structures to achieve carbon neutrality. To address the load problems of existing soil-based ecological systems, a lightweight system utilizing microalgae bioreactors was proposed, and structural performance was evaluated for four types of large-span structures: truss, arch, dome, and cable structures. Structural analysis results through finite element analysis showed that the proposed system achieved a 70% load reduction effect compared to existing systems, with structural performance improvements including 35-40% reduction in maximum deflection, 30-35% reduction in maximum stress, and 25-30% increase in natural frequency. Environmental performance analysis confirmed CO₂absorption capacity of 12-18 kg per m² annually and PM2.5 reduction effects of 15-25%. Economic analysis results indicated that benefits of 3.95-6.7 million KRW per year are generated for a 1,000 m²reference area, creating cumulative benefits of 179.75-227.5 million KRW over 25 years. Verification through the German BIQ House case confirmed CO₂reduction performance of 6 tons per year for 200 m², demonstrating the practical applicability of the system. This study presented the potential of an innovative ecological integration system that can ensure structural safety of large-span structures while simultaneously contributing to carbon neutrality.

Endocrine-disrupting chemicals (EDCs), such as perfluorooctanoic acid (PFOA), have been linked to adverse effects on reproductive health. However, the impact of PFOA exposure during the embryonic stage on hypothalamic gonadotropin-releasing hormone (GnRH) neurons, which are central to the regulation of the hypothalamic–pituitary axis, that controls the reproductive system, has not been investigated. In this study, immortalized embryonic mouse hypothalamic cells (mHypoE-N46) were used to evaluate alterations in GnRH expression following PFOA exposure in vitro. In addition, the expression levels of Pnx, Gpr173, BDNF, and Ntrk2—upstream signals known to regulate GnRH expression—were examined. mHypoE-N46 cells were treated with PFOA for 2-24 hours, and gene expression and protein expression levels were evaluated. PFOA significantly altered the gene expression levels of Pnx, Gpr173, BDNF and Ntrk2. GnRH expression was significantly increased at both the mRNA and protein levels. In conclusion, exposure to PFOA may perturb the upstream signaling of GnRH and may directly or indirectly affect the expression of GnRH, suggesting that PFOA may alter the GnRH regulatory network.

This study proposes empirical formulas for predicting the nonlinear behavior of GIR beam-to-column connections in timber structures to evaluate their structural performance. A database comprising 59 experimental results of GIR connections was collected, and the normality of data distribution was verified. Statistical analysis were conducted to investigate the correlations between input and output parameters. Based on input parameters with high correlation, derived variables were formulated and utilized in a multiple regression analysis to develop empirical formulas for moment capacity and rotation. The R-squared values of the proposed formulas exceeded 0.9, and the predicted initial stiffness and strength closely matched those of experimental results not used in the regression analysis. So the suggested empirical formulas exhibit excellent predictive performance for the nonlinear behavior of GIR beam-to-column connections in timber structures.

This study quantitatively evaluated the ghost fishing effects caused by lost gillnets and pots through abandoned fishing gear retrieval surveys conducted along the east and south coasts of Korea. The catch efficiency of retrieved gear was compared with commercial gear, and Monte Carlo simulations were employed to estimate ghost fishing rates over time. The results showed that the ghost fishing rate of snow crab gillnets and spring-type whelk pots retrieved from the East Sea were 50.7% and 57.9%, respectively while the drum-type octopus pots from the South Sea showed a ghost fishing rate of 48.3%. These findings indicate that lost fishing gear retains more than half the catch efficiency of commercial gear, and the impacts of ghost fishing can persist over extended periods. Simulation results revealed a sharp decline in ghost fishing rates within the first three months of submersion, followed by a gradual decrease, confirming the long-term impact of ghost gear. When applying time-weighted ghost fishing rates based on soak duration, the estimated loss in 2023 from ghost fishing in gillnet and pot fisheries amounted to approximately KRW 419.9 billion, representing 9.62% of the total production value of coastal and offshore fisheries. These findings emphasize the need for mandatory use of biodegradable gear, regular seabed gear monitoring, and the implementation of traceability and gear identification systems. The study provides scientific evidence for the development of policies and technical standards aimed at mitigating ghost fishing.