A field study was conducted to reduce airborne bacteria by supplying active ions to indoor spaces used by vulnerable human groups spending substantial amounts of time in places such as schools and hospitals. In an experiment conducted during school hours (8:00-15:00), the average number of airborne bacteria in classrooms was 345.53 CFU/m3 or more without active ions. However, ion supply reduced the airborne bacteria to an average of 113.23 CFU/m3, indicating an efficiency of 61.61%. As a result of tests in 33 rooms used for surgery in small and middle sized hospitals, ion supply for 2 to 4 hours reduced the average airborne bacteria concentration from 243.88 CFU/m3 to 104.34 CFU/m3, representing a 41.53% reduction. A laboratory test to confirm the ion activity has shown that the mortality rate of E. coli used as a test bacterium increased with exposure time to ions. The initial colony number of E. coli was 251 CFU, but decreased to 4 CFU after 60 minutes of exposure to active ions. Therefore, it was confirmed that the supply of active ions can contribute to the control of airborne bacteria in the indoor environment of schools, hospitals and other public facilities.

The present study was conducted to examine the effect of soybean silage as a crude protein supplement for corn silage in the diet of Hanwoo steers. The first experiment was conducted to evaluate the effect of replacing corn silage with soybean silage at different levels on rumen fermentation characteristics in vitro. Commercially-purchased corn silage was replaced with 0, 4, 8, or 12% of soybean silage. Half gram of the substrate was added to 50 mL of buffer and rumen fluid from Hanwoo cows, and then incubated at 39°C for 0, 3, 6, 12, 24, and 48 h. At 24 h, the pH of the control (corn silage only) was lower (p<0.05) than that of soybeansupplemented silages, and the pH numerically increased along with increasing proportions of soybean silage. Other rumen parameters, including gas production, ammonia nitrogen, and total volatile fatty acids, were variable. However, they tended to increase with increasing proportions of soybean silage. In the second experiment, 60 Hanwoo steers were allocated to one of three dietary treatments, namely, CON (concentrate with Italian ryegrass), CS (concentrate with corn silage), CS4% (concentrate with corn silage and 4% of soybean silage). Animals were offered experimental diets for 110 days during the growing period and then finished with typified beef diets that were commercially available to evaluate the effect of soybean silage on animal performance and meat quality. With the soybean silage, the weight gain and feed efficiency of the animal were more significant than those of the other treatments during the growing period (p<0.05). However, the dietary treatments had little effect on meat quality except for meat color. In conclusion, corn silage mixed with soybean silage even at a lower level provided a greater ruminal environment and animal performances, particularly with increased carcass weight and feed efficiency during growing period.

Probiotic strain is known to regulate the immune system by colonizing in the intestine and interact with intestinal cell receptors of lymphoid tissue. In this study, safety of Streptococcus thermophilus KCTC14471BP and silk fibroin coating effects was evaluated with respect to mucin binding abilities and immune system modulation. S. thermophilus KCTC14471BP was coated with silk fibroin by adding 1% water-soluble calcium and 0.1% silk fibroin. S. thermophilus KCTC14471BP showed the high activities of leucine arylamidase and β-galactosidase. Regarding the antibiotic resistance tests, S. thermophilus KCTC14471BP was susceptible to ampicillin, vancomycin, gentamicin, kanamycin, streptomycin, erythromycin, clindamycin, tetracycline, and chloramphenicol. S. thermophilus KCTC14471BP coated with silk fibroin showed the improved mucin binding ability from 16.1% to 71.3% and was confirmed to have no cytotoxicity against RAW 264.7 macrophage. S. thermophilus KCTC14471BP coated with silk fibroin showed dose-dependently significant increases in pro-inflammatory cytokines IL-1β, IL-6, and TNF-α. These results suggested that S. thermophilus KCTC14471BP can be expected as a promising probiotic bacteria for applications to food industries such as fermentation or functional foods.

본 연구에서는 2021년 전남, 전북을 대상으로 23개소에서 어류 4종(향어, 비단잉어, 참붕어, 금붕어) 총 1,115마리에 대하여 병원체 감염 여부를 조사하였다. 법정전염병 3종(SVC, Spring viraemia of carp, EUS, Epizootic ulcerative syndrome, and KHVD, Koi herpesvirus disease)을 분 석한 결과, 조사한 모든 시료에서 법정전염병이 검출되지 않아 전라권에서 조사한 잉어과어 류는 청정국 또는 청정지역 선언에 중요한 정보를 제공할 것이다.

PURPOSES : The purpose of this study is to perform traffic flow characteristics analysis for each point of the long-term work zones and to propose an estimated capacity method to support the establishment of traffic flow management strategies for the long-term work zones. METHODS : The study explained the difference in traffic flow characteristics between the short-term and the long-term work zones, and estimated the capacity of the long-term work zones. The analysis data were collected from five points of long-term work zones of the twolane highway. And VDS and DSRC data were applied to validate data. RESULTS : The characteristics of traffic flow at each measurement point in the long-term work zones showed some differences, among which the capacity was estimated as the starting point to be 1,200 pcphpl and the ending point, 1,400 pcphpl. The delay length was estimated by applying the queuing theory based on the capacity of the start point where the bottleneck starts. As a result of verifying the congestion length based on space diagram map analysis, it was determined that the capacity calculation value and estimation methodology presented in this study were appropriate. CONCLUSIONS : The long-term work zones are mixed with different characteristics of roads, so as the capacity values depending on the analysis location. Therefore, it is necessary to select analysis points and methodologies for estimating capacity and delay depending on the purpose of the analysis, such as estimating the maximum queue length or analyzing the maximum travel time. Through this study, it is expected that by providing accurate information on congestion in advance, road users can detour to other roads, and construction officials can adjust the construction plan to minimize congestion in the construction section.

The purpose of this study was to examine the quality characteristics of byeolmijang prepared several roasted whole grain powders (oat, brown rice, black soybean, corn) for eight weeks. As the fermentation progressed, the pH decreased from 6.10~6.12 to 4.48~4.92 and the total acidity increased dramatically from 0.41~0.48% to 1.67~2.24%. There were no differences in the moisture content. The content of reducing sugar decreased, in particular, brown rice sample(C) decreased significantly than the other samples during fermentation. In color, L and b-value decreased all samples, whereas a-value showed a tendency to slightly increase. The total cell counts and lactic acid bacteria revealed an increasing tendency during fermentation. In case of the amino-type nitrogen contents, it increased significantly during the fermentation period, especially control sample (A) showed the highest content significantly. The total polyphenol of all samples increased in the fermentation period. ABTS and DPPH radical scavenging activities also increased, especially corn sample (E) had the highest levels. In by electronic tongue analysis, corn sample (E) revealed higher umami and sourness than the control. So, by adding roasted corn powder, it can enhance function and taste of byeolmijang.

This study was carried out to investigate the effect of side vent heights on temperature and relative humidity inside and outside the single-span plastic greenhouse (W: 7 m, L: 40 m H: 3.9 m) during natural ventilation. Four different heights (120, 100, 80, 60 cm) of the side vent were used as an experimental condition. Variations of temperature and relative humidity inside and outside the greenhouse and the differences between heights were compared by using one-way ANOVA. In the daytime, the difference in temperature between inside and outside the greenhouse was dropped from 14.0°C to 7.1°C as the side vent height increased. The temperature difference in the nighttime was less than 0.2°C regardless of the height. One-way ANOVA on the temperature difference between heights presented that the statistical significance was founded between all of the combinations of height in the daytime. The difference in relative humidity between inside and outside the greenhouse was grown from –13.8% to – 22.2% with a decrease in the side vent height. The humidity difference in the nighttime was less than 1% regardless of the height. One-way ANOVA on the humidity difference revealed that most of the side vent heights showed significance in the daytime but between 100 and 80 cm was not significant. It seemed because the external air became cooler during the experiment with a height of 80 cm. Conclusively, this study empirically demonstrated that the higher side vents resulted in the decrease of differences in temperature and relative humidity between inside and outside the greenhouse, and also the effect of side vent height was statistically significant. This study may be helpful for deciding the height of the side vent effective for controlling temperature and relative humidity in a single-span greenhouse during natural ventilation.

Known for its effectiveness in weight loss and diabetes prevention, Gymnema sylvestre products can be found in the US, Japanese, and Indian markets. However, the recommended dosage or safety of these products has not yet been proven. Therefore, development of an analytical method for detecting the content of Gymnema sylvestre in food products is required. Accordingly, this study proposes an analysis method that can examine Gymnema sylvestre in food using LC-ESI-MS/MS and KASP (Kompetitive Allele-Specific PCR) markers. In LC-ESI-MS/MS, a simultaneous analysis method for gymnemic acid and deacylgymnemic acid was optimized using negative ionization mode, and its validation test was completed for solid and liquid samples. In addition, KASP markers were prepared by finding the specific SNP of G. sylvestre in ITS2 and matK through DNA barcodes. The two KASP markers returned positive FAM fluorescence result when combined with G. sylvestre, and this aspect was confirmed in raw G. sylvestre as well. The applicability of the method was tested on 21 different food and healthy functional products containing G. sylvestre purchased on the internet. As a result, although there was a difference in the ratios of gymnemic acid and deacylgymnemic acid in LC-ESI-MS/MS, the index component was detected in all 21 products samples. In the KASP analysis, 9 products returned positive FAM result, and the rest of the products were found to be containing G. sylvestre extract. This study is the first study to use the dual system of LC-ESI-MS/MS and KASP for the analysis of G. sylvestre. The study has confirmed that these two methods are applicable to the examine G. sylvestre content in food products.

본 연구는 나라꽃으로서 상징성이 높은 무궁화를 실내공간에 도입하기 위하여 실내광원별 경관적 특성을 살펴서 효율적인 도입방안을 모색하기 위해 수행되었다. 공시식물로서 무궁화 품종 중 내음성이 비교적 강하다고 알려진 무궁화 “백단심” 을 선정하였으며 형광등, LED등, 삼파장등, 광섬유 4종류의 광원과 500, 750, 1500, 2,500lux의 광도에서 7개월간 실험하였다. 식물의 경관적 측정요소로 엽장, 엽폭, 초장, 엽수, 낙엽수, 절간장, 엽록소함량 등을 조사하였다. 식물의 생리적 요소로서 광합성률(Pn), CO2흡수율(CO2), 세포내 CO2농도(Ci), 대기중 CO2농도(Ca), 기공전도도(Cs), 증산량(Tr) 등을 측정하였다. 또한 기공수를 관찰하여 광합성이 잘 이루어지고 있는지 확인하였으며 광원별 개화율과 색차계를 이용한 화색을 조사하였다. 실험결과 및 도입방안은 다음과 같다. 첫째, 식물의 경관적 구성요소 중 엽수가 모든 광원에서 통계적 유의성을 보였다. 엽수는 엽장, 엽폭과 함께 수관 형성 및 수형에도 영향을 미치는데 광도가 높을수록 많이 출엽하였으며, 광섬유 1,500lux 하에서 가장 많이 출엽하였다. 엽장 엽폭은 광도가 높을수록 크기가 커지는 경향을 보였다. 둘째, 무궁화는 잎도 특이하지만 꽃이 아름답고 상징성이 있어서 선호되는데 무궁화 “백단심” 은 8월~10월에 개화한다. 꽃을 감상할 목적으로 광원별 광조건을 살펴보면, 형광등 750lux~2,500lux, LED등 1,500lux~2,500lux, 삼파장등 750lux~2,500lux, 광섬유1,500lux~2,500lux로 파악되었다. 가장 효과적인 광원 및 광도는 광섬유 1,500lux~2,500lux인 것으로 나타났다. 셋째, 잎의 신선도는 광합성량 및 잎의 기공의 특성으로도 가늠해 볼 수 있는데 광도가 높을수록 광합성률은 높아졌다. 광원별 기공의 수는 7~28개로 나타났으며 LED등 1,500lux와 광섬유 1,500lux에서 가장 많은 기공수를 보였다. 넷째, 색차계 분석결과 꽃색은 분홍빛 보다는 옅은 Green에 가까우며, Blue에 가까운 색상을 나타내었다. L*(명도)의 값은 90 이상의 수치를 나타내어 명도가 매우 높은 것으로 조사되었다. 최종적으로 생육상태 및 생장량, 개화율을 고려할 때 무궁화 백단심의 최적광원은 광섬유 1,500lux인 것으로 판단된다. 그러나 향후 더 많은 무궁화 품종을 대상으로 적정광원과 적정광도를 파악하고 무궁화 연령별 개화율에 대한 실험을 추가적으로 하여 나라꽃 무궁화의 활용가치 및 활용 영역을 향상할 필요성이 있다.

This study was performed to evaluate the effect of pork belly thickness on physicochemical and sensory characteristics after pan frying. Pork belly with three different thickness (5, 10, 20 mm) from three different parts (A:thoracic vertebrae no. 5-9, B:thoracic vertebrae 10-14, C:lumbar vertebrae 1-6) was pan grilled with electronic grill until it reached an internal temperature of 71℃. Cooking loss, shear force, sensory characteristics, volatile compounds of cooked pork belly were determined. Electric nose analysis was performed to support descriptive analysis and consumer sensory analysis. The cooking loss of pork belly with 20 mm thickness from all positions was significantly low (p<0.05). Shear force increased with increase in thickness (p<0.05). Electric nose identified major seven volatile compounds such as acetonitrile, (Z)-4-heptenal, 2-octanol, linalool, carbon disulfide, methylcyclohexane, n-nonanal to discriminate those samples. The part of pork belly significantly affected the intensity of volatile compounds rather than thickness. In discriminate analysis, the overall acceptability of pork belly (A and B parts) with 20 mm thickness was higher than that of pork belly with 5 mm thickness. In addition, the overall acceptability by consumers for pork belly with 20 mm thickness from A, B, and C parts was higher than the overall acceptability for pork belly with 5 mm thickness. This preliminary study suggests that it is more desirable to use pork belly with 20 mm thickness from part A when cooking on a pan gril compared with pork belly of 10 mm or 20 mm thickness from part B or C in the meat markets and restaurant.

보호지역 중 국립공원은 생물다양성이 풍부한 곳으로 다른 곳에 비해 인간에게 제공되는 생태계서비스 혜택이 높은 편이다. 이러한 국립공원의 가치를 객관적이고 과학적인 데이터를 기반으로 관리하기 위해 생태계서비스 평가가 활용되 고 있다. 생태계서비스는 공급, 조절, 문화, 지지의 4가지 서비스로 분류되며, 본 연구에서는 지지서비스 중 서식지질을 평가하였다. 서식지질 평가는 InVEST의 Habitat Quality 모델을 활용하였으며, 선행연구 분석 및 국내 보호지역 실정을 반영하여 인자별 민감도 및 서식지질 초기값 계수를 재설정하였다. 한라산국립공원을 제외한 21개 국립공원의 서식지 질을 분석하고 지도화하였다. 서식지질은 0과 1사이의 값으로 나타나며, 1에 가까울수록 자연성이 높은 것으로 평가한 다. 서식지질 분석결과 설악산·태백산국립공원(0.90), 지리산·오대산국립공원(0.89), 소백산국립공원(0.88) 순으로 높 게 나타났다. 해안-해상형 국립공원을 제외한 18개 국립공원의 면적과 서식지질을 비교한 결과 면적이 넓을수록 전반적 으로 서식지질이 높게 나타났다. 용도지구별 서식지질을 비교한 결과 공원자연보존지구, 공원자연환경지구, 공원문화 유산지구, 공원마을지구 순으로 서식지질이 높게 나타났다. 서식지질 분석과 용도지구별 법적인 규제를 함께 고려하였 을 때, 인위적인 행위가 제한될수록 서식지질은 높아지는 것으로 판단된다. 본 연구는 국내 보호지역 상황에 맞게 매개변수를 조정하여 21개 전 국립공원을 대상으로 서식지질 분석을 실시한 것에 의의가 있다. 적확한 수치와 지도화를 통해 직관적으로 파악이 용이하며, 향후 보호지역의 개발 및 보전에 관련한 정책 결정에 유용할 것으로 기대된다.