This study was carried out to establish the consumer-centric food labeling system by investigating usage state, importance evaluation, problems and satisfaction, etc. on the current food labeling system by consumer. For usage state of checking the food label, 50.2% of respondents were replied ‘mostly check the label’, and they were indicated a significant difference on ‘education level(p<.05)’, and in case of married respondents, most were relied 'absolutely check the label', For the reason to check the food label, 61.8% of respondents were replied 'to determine whether it is stable or not', and they were indicated a significant difference on age(p<.01), marital status(p<.01), and job(p<.01). For the item considered as important things on the label of the whole food, 49.8% of respondents were replied that 'expiration and manufacture date is important', and they were indicated a significant difference(p<.001) on age, marital status, job. For item considered as important things on the label of each food, it was indicated that they considered food company as important thing in case of snack, soft drink, edible oils, and noodles, and food company in case of ice cream, and expiration and manufacture date in case of milk product and meat product. For the problems on the current food labeling system, the clauses 'Poor marking on food additives and materials contents' and 'untrue marking of nutrition contents' were indicated a significant difference on age(p<0.05, p<0.001), and the clause 'untrue marking of nutrition contents' were indicated a significant difference on marital status(p<.01) and job(p<.01).

두부, 콩나물, 된장에서 유전자재조합 대두의 혼입여부를 판별하기 위해 가장 적합한 PCR 프라이머의 선택과 생산 고정에서 물리?화학적인 변성을 재조합 DNA의 손실정도를 공정단계별로 비교 분석하였다. 내재유전자인 β-actin은 600, 495, 250, 160bp을 비교한 결과 160bp에서 가장 광범위하게 검출되었으며, 35S promotor는 130bp, NOS terminator 132bp의 작은 사이즈 프라이머가 유효하였다. 가공공정별로 유전자의 손실정도를 팡가한 결과 두부는 대부분 DNA가 잘 보존되어 가공공정에 따른 DNA의 손실은 거의 관찰되지 않았다. 콩나물은 대부분의 DNA가 발아직후 줄기로 이동하여 적절한 분석부위는 줄기로 판단되었으며, 된장은 효소의 작용에 의하여 유통기간내에 DNA의 검출차이를 보였다. 20일 후 삽입유전자는 소실되었고 특히 50일 이후에는 대부분의 내재유전자 뿐만 아니라 분해되어 검출이 어려운 것으로 판단되어다. 가공처리조건인 열에 의한 DNA의 변성은 100℃에서 40분간 가열하여도 DNA의 손실되지 않고 보존됨을 알 수 있었다.

PCR법은 특정 유전자를 증폭하는 기술로 작물 및 식품에서 유전자 변형체 함유 여부를 가리는 효과적인 방법이다. 그러나 핵산의 추출법에 따라 PCR의 감도가 크게 달라지므로 정확한 추출법의 선정이 매우 중요하다. 본 연구는 현재 컬럼형 상용화 키트와 기존의 용매 추출방법을 이용하여 콩과 옥수수가공식품에 대한 각 유전자 부위의 검출감도를 비교 분석하였다. 핵산의 추출효율과 도입유전자의 증폭효율면 모두 상용화 키트인 Wizard™, DNeasy™ 추출법이 우수하였다. DNeasy법은 대부분의 식품에서 우수한 추출효율을 보였으나, 옥수수가공식품에서 수율이 감소하는 단점을 나타내었다. Wizard법은 모든 가공식품에서 고른 추출효율을 보였으며, PCR반응에 의한 증폭산물도 잘 보존되어 가공식품의 GMO검출에 적합한 것으로 나타났다. 한편 CTAB법은 콩가공식품에서 약간 효율이 좋은 것으로 나타났으나 대부분의 경우 효율이 낮았으며, 식품의 종류에 따라 편차가 심하게 나타났다. pheno/chloroform법은 대부분의 식품에서 핵산의 분리가 어려운 방법으로 나타나 GMO분석에는 적합하지 않은 방법으로 확인되었다.

This study was conducted to determine the content of heavy metals in canned foods and soft drinks available on the Korean markets. Trace metals (Pb, Cr, Cd, and Sn) were detected in 24 kinds, 120 samples by Atomic Absorption Spectrophotometer. The average concentration of heavy metals in canned foods was in the order of Sn (6.930 ppm)$gt;Cr (0.050 ppm)$gt;Pb (0.030 ppm)$gt;Cd (0.008 ppm), which was the same order in soft drinks as Sn (3.519 ppm)$gt;Cr (0.080 ppm)$gt;Pb (0.024 ppm)$gt;Cd (0.001 ppm). The total contents of heavy metals in canned fruits and fiuit juices were relatively higher than those in cans and drinks made of vegetable and fish. It can be supposed that the high acidity owing to the organic acid of fruit itself promotes to extract metals from can materials, and although fish usually contains more heavy metals than vegetables, canned fish revealed low level because internal organs and most of skin which had more heavy metals than meat were removed throughout canning process. Because processed foods such as canned foods and soft drinks are very popular with the children and adolescence according to the change of life style and eating habit, and the possibility of exposure to heavy metals by the habitual intake of these is increasing simultaneously, it is suggested that more practical study about the process of exposure and the amount in each step is needed for the assessment of safety.

Commonly eaten fish, meat and other protein-containing foods show some level of mutagenic activity following normal cooking such as broiling, frying, grilling, roasting etc. The main food mutagens found in cooked products are $quot;heterocyclic aromatic amines$quot; . Several of them have been shown to be carcinogenic in rodent and suggested to be relevant for human cancer etiology. This review summarizes the chemistry, formation, occurrence and toxicity of food-borne heterocyclic aromatic amines. Factors that influence the formation of them are also discussed with special emphasis on dietary factors. From a health safety point of view, it is desirable to estimate the intake of heterocyclic amines via foods, and reduce or prevent the formation of food mutagens.

한우육을 비롯한 육류 4종, 육가공식품으로서 다시 다류 4종, 라면수프류 5종, 컵라면수프류 2종, 그리고 햄류 3종과 소시지류 3종에 있어서의 크레아티닌 함량을 조사하여 다음의 결과를 얻었다. 한우육에 있어서의 크레아티닌 함량은 시료 100g당 252.0㎎으로서 수입우육의 경우보다 높았으며, 계육<돈육<수입우육<한우육의 순으로 함량이 높게 나타났다. 다시다류와 라면수프, 컵라면수프류에서의 크레아티닌 함량은 2.0㎎에서 67.4㎎까지의 분포를 나타내었으므로 이들의 조성성분중에 모두 육류성분이 존재하는 것으로 나타났다. 다시다류와 컵라면수프류중의 한가지 시료는 다른 3종의 다시다류나 1종의 컵라면수프류의 크레아티닌 함량보다 매우 높았다. 햄류와 소지지류의 경우 크레아티닌 함량이 고른 분포를 보였으며, 햄류의 크레아티닌 함량이 소시지류보다 22∼37% 정도 높았다.

This study was carried out to give some help for housewives recognizing the desirable purchase of processed foods and food additives. This results were abtained as follows; In case of purchase action for processed foods of housewives confirmation of manufacturing date was the highest point(4.62). When housewives bought processed foods they considered the taste of them as first, and frequencies of processed foods intake showed the ratio of 46.6%, also it was shown to be used once a week. The order for use of processed foods were milk, milk products(butter, cheese, yoghurt, lactic bacterium beverage) 40.2% >canned foods 22.6% >meat products(ham, sausage, bacon) 20.9% > jelled fish 16.3%. Most of housewives wanted to have the knowledge and information for food additives at the highest point(4.11). The interest about food additives of housewives was shown to the order of synthetic preservative 3.86 >chemical seasoning 3.74 >synthetic sweetener 3.59 >synthetic coloring agent 3.53. Also, it was shown to be thought that the hazard factors effected on food safety were agricultural chemical residue(30.9%), environmental pollution(25.2, food additives(23.0%), and microorganism contamination(20.9%).

This study was undertaken to evaluate microbial risk degree of some processed foods in Korea. In this study the data on the outbreak of foodborne diseases during recent 18 years (1976-1989, 1993-1996. 8) were analyzed. The most frequently isolated pathogens were Salmonella (36.9%); followed Vibrio (22.0%), Staphylococcus (15.7%) and Escherichia coli (13.3%). Outbreak rate of Staphylococcus, Vibrio, E. coli and Salmonella, was 33.0%, 23.5%, 17.5% and 17.1%, respectively. Overall risk degree of pathogens by fatality rate, outbreak rate and pathogen amount for foodborne outbreak was Clostridium, 5, Staphylococcus and Vibrio, 4, Salmonella and E. coli, 3. Based on foodborne pathogens, the risk degree of raw seafoods, raw eggs and processed seafoods were 4, and those of raw meats, Doshiraks and milk products were 3. Also, based on processing characteristics of foods, the risk degree of surimi-based imitation crab was 3. Foods of the highest actual risk degree were raw seafoods and raw eggs (16); followed raw meats (15), surimi-based imitation crab (12), Doshirak (9) and milk products (6).

The purpose of this study is to establish the one serving size of Korean Processed Food. Defining the one serving size is very important for nutrition labeling and foodservice operation, because the one serving size is used to set up a proper portion by each foodservice operation. The basic data of 200 items were collected through three methods. Searching many cookbooks, exploring the commercial and noncommercial foodservices -6 industrial foodservices, 100 nationwide elementary school foodservice recipes analysis, and 3 hospital foodservice systems as the samples - moreover, experimental cooking and sensory evaluation by trained panels were conducted to assess quantity preference of selected food items. All data were rearranged through food type, that is, main dish, side dish, dessert and health food. One serving sizes of processed foods showed wide variety according to the different menus that include selected food items. Therefore, means and ranges of serving size by three research methods were presented item by item. The results obtained were: 1. The Korean Processed Foods were dried and sugar adding and soused foods, and many of them used the natual processing methods. 2. There were wide varieties in the classification of main dishes, but many of them were cereals, noodles, and sugar products. One serving size of noodles were around 50~100 g, cereals were 20~40 g, which means the one serving size can be differenciated by the food usage. 3. According to the Food classification of side dishes, many of them were as following; natural dried foods, processed fish products, salted or sugar added foods, seasoned foods and sugar products. Moreover the Types of cooking in side dishes were almost culinary vegetables, teas, health foods and condiments, and soused fish products. 4. About desserts, they were almost teas and sugars, and the Types of cooking were teas, health foods and seasonings. 5. We can conclude that almost Korean Processed foods used the drying and soused processing methods for long-time preservation, but it can make the higher content of any special elements, such as sodium or carbohydrates.

The major purpose of the present study is to survey vegetables, meats, seafoods, processed foods and imported foods for the presence. of Listeria spp. and to prevent listeriosis caused by the contamination of Listeria monocytogenes. Listeria spp. was isolated from 6.0% of meats, 3.4% of seafoods, and 11.4% of imported foods but was not found in vegetables and processed foods. The optimum growth condition of isolates indentified as Listeria monocytogenes was pH 7.0 and 37℃. The antimicrobial effect of grapefruit seed e×tract (GFSE) was observed in the level of more than 100ppm by disk method. When lml(2.5× 10^6 CFU/ml) of Listeria monocytogenes was inoculated and incubated for 3 days at 30, the total cell number of the organism was 4.5 × 10^9 in the control, 7.2 × 10³ in 100 μg/ml of GFSE medium, and 3.5 μg/ml of GFSE medium. Direct visualization of microbial cells by using both transmission electron microscope and scanning electron microscope showed microbial cell membrane the function of which was destroyed by treating with the dilute solutions of GFSE. It could be confirmed that GFSE completely inhibited the growth of the test strain of Listeria monocytogenes.

We analysed 398 kinds of processed foods sold on market - 43 kinds of ramyun, 67 kinds of bread, 104 kinds of cookies, 5 kinds of pizza, 28 kinds of sausage, 31 kinds of steamed fish cake, 4 kinds of tomato ketchup, 30 kinds of canned goods, 82 kinds of beverage and 4 kinds of danmoogi - to know mineral content such as P, Ca, Mg, Na and K. We used atomic absorption spectrophotometry for this study and the results were as follows; 1) P content was 204.24㎎% in sausages, 101.80㎎% in pizza, 67㎎% in canned fish, 44.50㎎% in canned vegetable, 38.62㎎% in ramyun and 32.10㎎% in steamed fish cake. Sausage had the highest P content showing almost 6 times as much as steamed fish cake. There was little difference between ramyun and steamed fish cake. 2) Ca content was 115.00㎎% in pizza, 106.94㎎% in steamed fish cake, 100.59㎎% in sausage, 91.25㎎% in canned vegetable, 79.84㎎% in bread and 78.97㎎% in ramyun. 3) Mg content was 555.16㎎% in steamed fish cake, 476.14㎎% in cookies, 436.75㎎% in ketchup, 394.00㎎% in canned vegetable, 346.95㎎% in ramyun and 341.40㎎% in canned fish. Steamed fish cake was highest in Mg content and there was little difference between ramyun and canned fish. 4) Na content was 730.49㎎% in ramyun, 680.25㎎% in danmoogi, 548.43㎎% sausage, 463.50㎎% in canned vegetable, 449.25㎎% in ketchup, 401.29㎎% in steamed fish cake and 366.00㎎% in pizza. Ramyun had the highest Na content. 5) K content was 302.30㎎% in pizza, 280.25㎎% in ketchup, 219.68㎎% in sausage, 219.2㎎% in canned fish, 183.75㎎% in canned vegetable, 166.49㎎% in ramyun and 122.77㎎% in cookies. Pizza had the highest K content.