Background : This study examined the effect of harvesting time on the growth, yield characteristics, and major beneficial components in Salvia miltiorrhiza. Methods and Results : Although plant height, stem diameter and branch length were not affected by harvesting time, the number of stems was highest when harvested in mid October. There were no differences in root length and thickness, however, the rhizome was thicker when it was harvested at the end of October or early November than when it was harvested in early and mid October. The dried root weight also showed a similar pattern. However, there was a statistically significant increase to 408 ㎏ (16%) in the rhizome weight when in late October and a rise to 455 ㎏ (29%) when harvested in early November. Harvest time had little effect on the content of the major component of S. miltiorrhiza. For example, salvianolic acid content rose from 9.42 to 9.64% with later harvest times, and tanshinone ΠA content was tended to be slightly more increased in mid October which S. miltiorrhiza has 0.22% tanshinon ΠA than in early October. Conclusions : According to these results, the optimum harvest time for S. miltiorrhiza is early November when plant or major component yields are hightest. There were no significant harvest time effects on the major beneficial components.

Müllerian inhibiting substance (MIS) is a protein that encoded by MIS gene. It has also been called Müllerian inhibiting factor (MIF) and anti-Müllerian hormone (AMH). Mis expression occurs in ovarian granulose cells of females postpartum, and serves as a molecular biomarker for relative size of the ovarian reserve. In humans, the number of cells in the follicular reserve can be used to evaluate the reproductive function and fertility of female. Pagrus major is typical cultured fish in Korea but there is no clear evidence for their gene identification. However, in many teleost, MIS genes were demonstrated already. Present study aimed to identify the Mis gene in Pagrus major and seasonal difference of its expression. Using conserved sequence of the other known teleost Mis genes, we make conserved primers. Pagrus major’s ovary samples were obtained from the sea rim farm (Geoje, Korea) and kept in RNAlater®Solution or fixed in 4% paraformaldehyde containing 0.16% picric acid. RNA was isolated from kept sample and cDNA was synthesized. The PCR products were performed ligation with TOPO vector and transformation in TOP10 cell and sequenced the Mis mRNA fragment. MIS was localized in the follicle cells. Its mRNA levels were higher in summer than spring or fall. Based on them, it is suggested that MIS can be used to estimate the fertility of this fish.

High temperature is one of major environmental stress. Heat tolerance managing is difficult through the phenotypic selection, so marker assistant selection (MAS) using molecular markers like as RAPD, SSR etc. was tried to select useful traits for heat tolerance. Fourteen SSR markers reported by previous research were selected for this research. We tried to evaluate 14 SSR markers for MAS using 31 useful wheat resources including 24 crossing line from Turkey, six Korean wheat cultivars and Chinese spring. The average of the number of alleles and PIC values in this study were 6.14 and 0.64, respectively. Two major clades and four sub clades were grouped by phylogenetic tree using UPGMA. Four Korean wheat cultivars were distinct from other Turkey resources in the phylogenetic dendrogram. From the results, we expected that these markers were able to adapt to screening wheat genotyping for heat tolerance.

In 2013, International Agency for Research on Cancer (IARC) concluded that outdoor air pollution is carcinogenic to humans, with the particulate matter component of air pollution most closely associated with sufficient evidence of increased cancer incidence by exposure to particulate matter component of air pollution. Motor vehicles are one of a major emission sources of fine particle (PM2.5) in urban areas. A large number of epidemiological studies have reported a positive association of morbidity or mortality with distance from the roadside. We conducted this study to assess the association of PM2.5 concentrations measured at roadside hotspots with those at adjacent residential sites using real-time PM2.5 monitors. We conducted real-time PM2.5 measurements for rush hour periods (08:00∼10:00 and 18:00∼20:00) at 9 roadside air monitoring Hotspot sites in metropolitan Seoul over 3 weeks from October 1 to 21, 2013. Simultaneous measurements were conducted in residential sites within a 100 m radius from each roadside air monitoring site. A SidePak AM510 was used for the real-time PM2.5 measurements. Medians of roadside PM2.5 concentrations ranged from 9.8 μg/m3 to 38.3 μg/m3, while corresponding median values at adjacent residential sites ranged from 4.4 μg/m3 to 37.3 μg/m3. PM2.5 concentrations of residential sites were 0.97 times of hotspot roadside sites. Distributions of PM2.5 concentrations in roadside and residential areas were also very similar. Real-time PM2.5 concentrations at residential sites, (100 m adjacent), showed similar levels to those at roadside sites. Increasing the distance between roadside and residential sites, if needed, should be considered to protect urban resident populations from PM2.5 emitted by traffic related sources.

Fibroin silk proteins from spider or silkworm are attractive biomaterials that are of particular biotechnological interest for industrial and medical purposes because of their unique physical and mechanical properties. In this study, we generated and characterized the transgenic rice plant expressing a spider silk protein. Spider silks have great potential as biomaterials with extraordinary properties. Here, we report the cloning and characterization of the major ampullate silk protein gene from the spider Araneus ventricosus. A cDNA encoding the partial major ampullate silk protein (AvMaSp) was cloned from A. ventricosus. An analysis of the cDNA sequence shows that AvMaSp consists of a 240 amino acid repetitive region and a 99 amino acid C-terminal non-repetitive domain. The peptide motifs that were found in the spider major ampullate silk proteins, (A)n, (GA)n, and (GGX)n, were conserved in the repetitive region of AvMaSp. Phylogenetic analysis further confirmed that AvMaSp belongs to the spider major ampullate spidroin family of proteins. Recombinant AvMaSp-R was degraded abruptly by trypsin. However, AvMaSp-R was stable at 100 °C for at least 30 min. Additionally, the AvMaSp-R was stable at pH values from 2 to 12 for at least 1 h. Taken together, our findings describe the molecular structure and biochemical properties of the A. ventricosus major ampullate silk protein and demonstrate its potential as a biomaterial.

Youth Training Facilities is a facility used for youth training activities. Youth Training Facilities is a facility that could be vulnerable to accidents. The purpose of this study is to minimize any accidents through analysing previous inspection data. Should avoid accidents that may occur during training activities through real-time management of the facility.

In this study database on the results of in-depth inspection was built and statistical analysis was performed on the major faults for concrete dams. using condition evaluation results for existing concrete dams.

본 논문은 디자인 전공 분야에서 학생들이 현장 경험을 통해 실무적인 기술을 습득할 수 있도록 돕는 지역사회경험학습인 CBL(Community Based Learning) 학습 모델을 고찰하고 그 질적 효과를 NCS(National Competency Standards)에서 제시한 지식, 기술, 태도의 관점에서 분석하는데 목적을 둔다. ‘디자인과 지역사회경험학습’ 과목은 동의과학대학교 디자인계열의 ‘커뮤니케이션 디자인 프로젝트’ 과목을 수강하는 2학년 학생들을 대상으로 개설되었다. ‘디자인과 지역사회경험학습’ 교과목으로 학생들은 ‘커뮤니케이션 디자인 프로젝트’에서 배운 전공이론과 지식을 실제 현장에 직접 적용해보고, 프로젝트 결과물을 수업 시간 중에 발표한다. 그리하여 시각 디자인 직무의 현장 실무에 대한 이해를 향상시키고 경험을 넓힐 수 있게 된다. 본 연구를 통해 현장 실무와 관련된 CBL은 시각 디자인 전공 학생들의 전공능력과 직무 관련 능력을 향상시키는 것으로 나타났다. 즉, NCS 기준에 의한 학생 일지 분석, CBL 관련기관 평가, 포트폴리오, 자격증 취득 결과 등을 통해, 본 논문의 교육설계와 그 설계에 따른 실행이 학생들의 전공능력과 현장실무 능력을 높이는데 기여하고 있음을 보여주었다.

본 연구에서는 극한강우를 고려한 산사태 및 토석류 재해 실시간 예측 및 대응을 위하여 필요한 원천기술들 중, 토석류 전이규준 개발 및 이를 통한 발생부 토사량 추정 기법, 그리고 전이 이후 토석류 확산의 정량적 예측 분석에 결정적으로 중요한 인자들인 연행 증가율 및 유변학적 인자들에 대한 입력값을 결정하는 기법에 대하여 연구하였다. 또한 본 원천기술들을 실시간으로 연계 및 적용 가능하도록, GIS(Geographic Information System) 및 Web 기반의 산사태 및 토석류 관련 D/B 시스템을 경기도 용인지역에 시범 구축하였다. 이 시스템은 클라우드 환경에서 산사태 및 토석류 유발인자 관련 지리 공간적 컨텐츠를 저장하고 게시하여 Web 상에서 D/B를 공동으로 활용하고 분석할 수 있다. 토석류 전이규준에서 발생부 토사량 추정으로 이어지는 연구는 두 가지 접근법을 통해 이루어졌다. 이는 지형학적 인자들을 바탕으로 통계학적인 규준값을 설정하는 접근법, 그리고 국내의 전형적인 사면파괴 형태인 강우침투로 인한 침윤전선(wetting depth)의 하강과 같은 실제 메커니즘을 고려하는 물리적 기반의 접근법 등이다. 한 편, 국내 과거 토석류 발생 지역 데이터들을 바탕으로 다중 회귀분석을 실시하여 DAN3D 모델을 통한 토석류 확산 예측 분석에서의 연행 증가율에 대한 추정식을 개발하였다. 본 연구결과들은 궁극적으로 국내의 실시간 산사태 재해 예·경보 및 종합적 리스크 평가 시스템 구축을 위한 핵심 구성 원천기술들로 활용될 수 있을 것으로 기대된다.

파프리카에 발생하는 병들을 방제하기 위하여 합성농약이 광범위하게 사용되어왔지만 최근에 수많은 농약사용의 부작용에 대한 관심이 증가 하고 있다. 파프리카 주요병인 잿빛곰팡이병, 줄기 및 과실썩음병, 역병, 균핵병, 시들음병을 방제하기 위한 미생물을 분리하고 특성을 파악하기 위하여 본 연구를 실시하였다. 지방산분석과 16S rDNA 염기배열은 이 연구에서 분리한 YKJ1가 Serratia marcescens 그룹에 속하는 것을 밝혔다. 특히, YKJ1의 16S rDNA 염기배열은 S. marcescens의 염기서열과 99% 상동성을 보였다. 광학현미경을 통해 YKJ1처리에 의해 병원균의 포자 발아 및 균사 생장이 저해됨을 확인 하였다. YKJ1처리는 팽윤균사와 같은 현저한 형태적 변화와 세포벽의 분해를 유발하였다. 역병균의 경우 유주자낭의 형성이 억제되었다. 본 연구에서 동정한 S. marcescens는 S. marcescens-YKJ1으로 부르고자 한다. 포장실험등과 같은 시험이 차후 더 요구되어지나 파프리카의 주요 병관리를 위한 생물적 방제제의 하나로 가치가 있을 것으로 생각된다.

시판 건조김의 일반성분, 아미노산, 지방산, 구성당 및 무기질 함량을 분석한 결과는 다음과 같다. 수분 함량은 5.67~7.43%, 조회분 함량은 8.01~8.95%, 조지방 함량은 1.54~2.25%, 조단백질 함량은 37.77~39.98% 및 탄수화물 함량은 43.83~46.24%으로 산지에 따라 유의적인 차이를 나타냈다. 구성아미노산 함량은 건조김 100 g 당 완도가 25,982.15 mg, 부산이 23,847.35 mg, 고흥이 21,499.83 mg, 서천이 20,190.80 mg의 순으로, 필수아미노산은 건조김 100 g 당 완도가 9048.28 mg, 부산이 8003.11 mg, 고흥이 7178.65 mg, 서천이 7156.80 mg의 순으로, 유리아미노산 함량은 건조김 100 g 당 완도가 4,545.44 mg, 부산이 3,526.64 mg, 고흥이 3,103.19 mg, 서천이 2,871.62 mg의 순으로 산지에 따라 유의적 차이를 나타냈다. 주요 지방산 조성은 20:5n3가 64.50~70.06%, 16:0가 8.79~10.29%, 23:0가 3.15~7.36%, 22:1n9가 1.86~3.09%으로 산지에 따라 유의적 차이를 보였고 전체 지방산의 78.30~90.80%를 차지하였다. 구성당은 건조김 100 g 당 galactose가 3,678.84~4,052.52 mg, fructose가 2,112.30~2,473.86 mg, mannose가 1,103.74~1,648.39 mg, glucose가 361.67~590.21 mg의 순으로 산지에 따라 유의적 차이를 나타냈다. 무기질 함량은 완도 및 부산 김이 K, Na, Mg, Ca, Fe, Zn, Mn, Cu 순으로, 고흥 및 서천의 김은 K, Na, Ca, Mg, Fe, Zn, Mn, Cu 순으로 산지에 따라 유의적 차이를 보였다.

Since then the Special Act on the Safety Control of Public Structures has been enforced, the precision safety Inspection result was being collected. but it was not performed to comprehensive analysis and D/B. In this study, statistical analysis was performed by utilizing the Precise Inspection for Safety of Dam.

Adsorption and desorption characteristics of the representative 10 kinds components consisting of gasoline vapor on activated carbon were investigated at the temperature range of -30℃∼25℃. The breakthrough curves of each vapors obtained by the Thomas model were well described the breakthrough experimental results of this study. The breakthrough times of each vapors were correlated with the molecular weight, density, and vapor pressure. The breakthrough times had greater correlation with boiling point than molecular weight and density. The slope of the breakthrough curve was a proportional relationship with the rate constant (k) of Thomas model expression. The higher the slope of the breakthrough curve, the rate constant was larger. The biggest slope vapor had the smallest adsorption capacity (qe). Adsorption and desorption characteristics of mixed vapor similar to the gasoline vapor were studied at room temperature (25℃). The mixed vapor consisting of 9 components; group A (pentane, hexene, hexane), group B (benzene, toluene), group C (octane, ethylbenzene, xylene, nonane) was examined. Group A was not nearly adsorbed because of substitution by group C, and the desorption capacity of group A was smaller than group C. The adsorbed substances were confirmed to be Group C.

PGA 함량 정보가 없는 유전자원을 활용한 다양한 용도의 감자 품종 개발은 괴경 내 PGA 함량을 높이는 비의도적 결과를 초래할 가능성이 있다. 이러한 배경에서 신품종에 대한 정밀한 PGA 함량 분석은 감자의 식품안전성 제고와 PGA 저 함유 품종 개발을 위한 교배 모부본 확보차원에서 중요하다. 본 연구에서는 도입품종인 대서, 수미와 최근 개발된 하령, 고운, 홍영, 자영 4품종을 대상으로 괴경 부위, 저장기간별 PGA 함량변화를 분석하였다. 그 연구 결과를 요약하면 다음과 같다.