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        검색결과 326

        141.
        2014.06 KCI 등재 구독 인증기관 무료, 개인회원 유료
        The aim of this study was to develop the production of crossbred pigs suitable to the Korean consumers’demand. Crossbred (Jeju native pig × Landrace) pigs (n=175) at the age of 200 days, approximately classified into three slaughter weight groups (59~95 kg, 96~110 kg and 111~148 kg) were used to investigate the carcass traits and retail cuts characteristics. We have chosen longissimus dorsi, triceps brachii, biceps femoris, semimembranosus muscles, shoulder butt, thoracic vertebra (5-11th) and last thoracic vertebra for investigations of aforementioned parameters. The results showed that the 111~148 kg group had larger loin area, higher scores for meat color, quality and carcass grade than the other remaining groups (p<0.05). Weights of shoulder butt, rib, picnic shoulder, loin, belly, tenderloin, ham retail cuts, lean meat, fat and bone increased with increasing carcass weight (p<0.05). Ratios of shoulder butt, picnic shoulder, tenderloin, ham, lean meat, skin and bone in the 59~95 kg group were higher than that of the other groups (p<0.05). Ratios of belly in the 96~110 kg group were higher than that in the 59~95 kg group (p<0.05) but similar to the ratios of the 111~148 kg group. Total length, longest length and shortest length of belly increased with increasing carcass weight (p<0.05). Additionally, the longest width and shortest width of belly in 59~95 kg group were lower (p<0.05) than that in the other remaining groups. Length of shoulder butt increased with increasing carcass weight (p<0.05) whereas, the width of shoulder butt showed no differences between the groups. Weights of triceps brachii of picnic shoulder, biceps femoris and semimembranosus muscles of ham increased with increasing carcass weight (p<0.05). Overall, the crossbred pigs of 111~148 kg group possessed better quality and carcass grade however, the yield of belly in 96~110 kg group was higher than the other groups. Based on the consumption preference of Korean consumers therefore, the slaughter weight of crossbred pigs at ≥96 kg could produce higher economic benefit.
        4,000원
        142.
        2014.06 KCI 등재 구독 인증기관 무료, 개인회원 유료
        This experiment was conducted to investigate effect of brine mineral water (BMW) on growth performance and properties of blood in weaning pigs. Treatments allotted were 0% (general water), 2%, 3% and 5% BMW. In growth trial, a total of 36 weaning pig barrows [(Landrace×Yorkshire)×Duroc] weaned after 21 days with an average initial weight of 5.38±0.89 kg were used. Each treatment had 3 replications of 3 pigs per pen in a randomized complete block design. Weaning pigs were investigated for growth performance, complete blood corpuscle count and blood biochemical assay. In results, growth performance of 2% and 3% treatment groups were significantly higher than other groups (p<0.05). In addition, high density lipoprotein cholesterol was significantly (p<0.05) higher in 2% group than other treatment groups. On the other hand, mean corpuscular volume of supplement of BMW treatment groups were significantly (p<0.05) increased than control. Therefore, this study suggests that supplementation of BMW could improve growth performance and level of red blood cell in weaning pigs.
        4,000원
        143.
        2014.04 KCI 등재 구독 인증기관 무료, 개인회원 유료
        MYC (v-myelocytomatosis viral oncogene homologue) is a regulator gene that encodes for a nuclear phosphoprotein. Porcine MYC gene was mapped on chromosome SSC 4p13 and is associated with a variety of functions such as cell proliferation and cell growth. MYC expression is coupled to a multitude of physiological processes and is regulated by hormones, growth factors, cytokines, lymphokines, nutritional status, development and differentiation. MYC is also involved in myogenesis, muscle hyperplasia and adipogenesis. In this study, we investigated SNPs in MYC gene and their association with economic traits in Duroc, Landrace and Yorkshire populations. We detected a single point mutation in exon 3 of porcine MYC gene as a change of T to C at 906 base (amino acid position 302, nonsynomous mutation of alanine) in MYC-N domain. MYC mutation (T906C) was significantly associated with age at 90 kg in these breeds, signifying that this mutation can serve as a selection marker for growth traits in pigs.
        4,200원
        144.
        2014.04 구독 인증기관 무료, 개인회원 유료
        For successful embryo implantation, the communication of the maternal endometrium with the conceptus trophec-toderm is required essentially. In pigs, conceptuses undergo morphological change in length to enlarge the physical contact area with the maternal endometrium and secrete estrogen to induce the maternal recognition of pregnancy during the peri-implantation period. Conceptus-derived estrogen prevents luteolysis by conversion in direction of PGF2α secretion from the uterine vasculature to the uterine lumen as well as it affects on expression of the uterine endo-metrial genes. In addition to estrogen, conceptuses release various signaling molecules, including cytokines, growth factors, and proteases, and, in response to these signaling molecules, the maternal uterine endometrium also syn-thesizes many signaling molecules, including hormones, cytokines, growth factors, lipid molecules, and utilizes ions such as calcium ion by calcium regulatory molecules. These reciprocal interactions of the conceptus trophectoderm with the maternal uterine endometrium make development and successful implantation of embryos possible. Thus, signaling molecules at the maternal-conceptus interface may play an important role in the implantation process. This review summarized syntheses and functions of signaling molecules at the maternal-conceptus interface to further understand mechanisms of the embryo implantation process in pigs.
        4,200원
        145.
        2014.04 KCI 등재 구독 인증기관 무료, 개인회원 유료
        적리를 동반하는 돼지 분변에서 용혈성 균주를 분리 동정한 후, 장 독소 생성 유 무 및 항생제 감수성 시험을 수행하였다. 분리된 용혈성 균주 B. cereus BY06의 gyrB 유전자 염기서열을 분석한 결과, B. cereus와 99% 유사성을 나타내었다. PCR법에 의한 장 독소 유전자 검출 시험에서 B. cereus BY06는 장 독소 분비 양성으로 판정됨에 따라 설사형 식중독 균임이 확인되었다. B. cereus BY06를 이용한 항균제의 감수성 시험 결과, penicillin G에는 내성을 나타낸 반면 cephalothin, vancomycin, clindamycin, fusidic acid, gentamicin, ciprofloxacin, tetracycline 및 rifampin에 대하여 감수성을 나타냈다. 본 연구를 통해 돼지 분변에서 분리된 B. cereus 균주는 설사를 유발하는 장 독소를 분비하며, penicillin G에 대한 내성을 확인하였다.
        4,000원
        146.
        2014.04 구독 인증기관 무료, 개인회원 유료
        The objective of this study was to investigate the relationship between fertility and protein pattern change using in vitro fertilization, analysis of sperm characteristics and two-dimensional gel electrophoresis in different pig types. In results, the viability and mitochondria integrity of sperm were higher significantly (p<0.05) but the portions of ac-rosome reaction was lower significantly (p<0.05) in Duroc and F1 (potbellied×PWG miniature pig) than PWG minia-ture. On in vitro fertilization to investigate fertility, the fertility of F1 semen war higher significantly (p<0.05) than in Duroc and PWG miniature pig. On the other hand, protein patterns showed similar function among the different boar semen. Especially, the heat shock 70 kDa 1-like and G patch domain-containing protein 4 were significantly (p<0.05) higher expressed in F1 than in Duroc and PWG miniature pig. The proteins associated with mitochondria in Duroc were significantly (p<0.05) higher expressed than in F1 and PWG miniature pig. The developmental rates to blastocyst stage of oocytes fertilized with sperm of F1 pig were significantly (p<0.05) higher than in PWG miniature pig. However, phosphoglycerate kinase 2 and zinc finger protein 431 were significantly (p<0.05) higher expressed in PWG miniature pig than in F1 and Duroc pigs. In conclusion, the results of the present study indicate that different proteins were expressed in different pig types, and were associated with a sperm functions and embryo develop-ment.
        4,000원
        147.
        2014.03 KCI 등재 구독 인증기관 무료, 개인회원 유료
        In porcine embryo culture, one of reactive oxygen species (ROS) is harmful factors that are made during in vitro culture. To decrease the detrimental effect of ROS on embryo development, superoxide dismutase, catalase and glutathione peroxidase could be used in the embryo culture. Out of these antioxidants, 7,8-dihydroxyflavone (7,8-DHF) was reported its antioxidant effects to prevent the glutamine-triggered apoptosis. Therefore, this study was performed to investigate the most appropriate concentration of 7,8-DHF in porcine embryonic development. For that, 5 different concentration (0, 0.1, 0.5, 1, 2 uM) of 7,8-DHF was supplemented in the porcine IVM media and then maturation and blastocyst formation rates were compared among 5 groups. In maturation rates of porcine oocytes, significant higher maturation rates was shown in the 1.0 uM group compared with another 4 groups (83.3 ± 2.1 vs. 80.7 ± 1.4, 79.8 ± 1.4, 78.3 ± 1.2, 79.4 ± 1.6), respectively (P<0.05). In the embryo culture, 1.0 uM group also showed the significant higher cleavage rates (76.8 ± 3.1 vs. 62.1 ± 5.0, 65.7 ± 4.0, 68.6 ± 3.7, 64.6 ± 4.0%) and blastocyst formation rates - (39.6 ± 4.0% vs. 28.6 ± 3.3, 31.1 ± 3.9, 29.3 ± 2.5, 39.6 ± 4.0, 26.4 ± 3.2%), respectively (P<0.05). There was no significant difference among 5 groups in the cell number of blastocyst (P<0.05). In conclusion, supplement of 1.0 uM of 7,8-DHF was effective to increase the porcine embryonic development competence as antioxidant to ROS.
        4,000원
        148.
        2013.12 구독 인증기관 무료, 개인회원 유료
        Here, we present an approach of blood plasma proteome profiling and their comparisons between the young and the adult pigs as prerequisite for the identification of bio-markers related to the health conditions, growth performance and meat quality. To profile the proteome in porcine plasma, blood samples were collected from 19 young piglets and 20 adult male barrows and the plasma was retrieved. Then, protein profiling was initiated using one and two-di-mensional electrophoresis. Proteins were spotted and then identified by MALDI-TOF-TOF and LC-MS-MS. In the re-sults, more than thirty-six and twenty eight protein spots were selected in young piglets and adult pigs, respectively and twenty three proteins were identified. The proteome profile images were compared between those ones using Image Master Version 7.0. The image of expressed proteome showed that most of proteins from plasma of young pig-let separated clearly and concentrated in 2DE display compared to ones from adult. Image analysis in detail was car-ried out to look for the specific proteins related to age progression. It demonstrated that the characteristics of proteome expression could be distinct to their age stages. Further investigations needed to proceed to understand the age de-pendent change of protein conformation and biological meaning of those differences in proteome expression between young and mature adult pigs.
        4,000원
        149.
        2013.12 KCI 등재 구독 인증기관 무료, 개인회원 유료
        본 연구는 식물성 불포화지방산 복합제로 이루어진 보조사료를 첨가하여 이유자돈의 균일성과 증체성적에 미치는 효과를 구명하기 위하여 수행하였다. 공시동물은 일반 종돈장의 Landrace 모돈 110두와 포유자돈 248두를 공시하였다. 시험구는 모돈시험에서는 장쇄지방산 복합제제를 분만사입식부터 이유시까지 약 4주간 급여하였고 자돈시험에서는 액상 중쇄지방산 complex를 포유기간 중 2회 구강투여 하였다. 모돈 시험에서 복당생존자돈수와 복당생시체중은 처리구간 차이를 보이지 않았으나, 복당이유자돈수와 복당이유체중에서는 시험구가 대조구에 비해 유의적으로 높았다(P<0.01). 모돈의 사료섭취량은 시험구가 유의적으로 높았으며, 발정재귀일령은 유의적으로 차이는 없었으나 시험구에서 평균 0.3일 정도 빠르게 나타났다. 포유기간내 일당증체량 역시 이유체중과 양의 상관관계를 갖고 있어 유의적인 차이가 있는 것으로 나타났다. 자돈시험 결과 시험구는 포유기간내 이유체중이 대조구에 비해 유의적으로 높았으며 생시체중 대비 증체량도 같은 기간에 많은 차이를 나타내었다. 자돈의 이유체중에 대한 변이의 지표를 보면 시험구와 대조구간 변이의 차이는 없는 것으로 나타나 개체간의 균일성에 대해서는 처리간차이는 나타나지 않았다. 결론적으로 포유기의 장쇄지방산 급여는 모돈의 젖에서 올레인산, 리놀산 등불포화지방산의 함량을 증가시키고, 젖 분비량과 사료섭취량을 증가시켜 자돈의 초기증체에 기여하는 것으로 나타났고, 자돈에 대한 중쇄지방산의 급여도 이유체중을 증가시키는 효과를 가져왔다.
        4,000원
        150.
        2013.10 KCI 등재 구독 인증기관 무료, 개인회원 유료
        본 연구는 244,443 도체로부터 얻은 도체특성(성별, 등지방두께 및 도체중), 사육환경(자돈의 성별 분리사육, 돈사의 온도관리 유무, 돈사의 형태, 환기방식, 급수원의 종류, 음수 개선시설의 유무, 돈사의 연간 소독횟수, 돈사의 바닥형태 및 평당 사육두수), 출하방법(선별출하, 출하일령, 등지방두께의 편차 및 도체중의 편차) 및 번식특성과 자돈관리(모돈의 산차, 이유일령, 정액의 분양처 및 자돈의 품종)등의 자료를 바탕으로 돼지의 도체가격에 미치는 영향을 분석하기 위해 실시하였다. 도체특성 변수에서 암퇘지 및 거세돈이 숫퇘지보다 높은 가격을 형성하였으며(p<0.05), 등지방두께 15-29mm 및 도체중 91-95kg가 높은 도체가격을 보였다(p<0.05). 도체가격에 대한 등지방 두께 및 도체중은 도체가격 형성에 각각 90.75%와 90.23%를 차지하였다. 하지만, 사육환경, 출하방법, 번식관리는 각각의 방법별로 가격에 유의적인 차이를 보였으나, 도체가격형성에는 낮은 영향을 미쳤다. 이상의 결과 여러 가지 변수 중 등지방 두께 및 도체중이 도체가격에 큰 영향을 미친 것으로 나타났다.
        4,000원
        151.
        2013.09 KCI 등재 구독 인증기관 무료, 개인회원 유료
        The aim of this study was to establish a three dimensional (3D) culture system of endometrial cells and to examine the plasminogen activators (PAs) activity in porcine uterine. The 3D culture system in porcine endometrial cells was composed to mixture 3D gel, stromal cells and epithelial cells. The 3D culture system was used to identify normal structure search as uterine tissue and PAs expression in this study. In results, porcine endometrium epithelial cells forming a top monolayer and endometrium stromal cells developed as fibroblast-like within 3D matrix scaffold. Expression of urokinase-type PA (uPA) and tissue-type PA (tPA) were observed during the 3D culture using immunofluorescence. PA activity in 3D-cultured endometrial cells was no significant difference between the tissue type, but 2D culture system were significantly lower than in 3D-cultured endometrial cells (P<0.05). Therefore, basic system and functional aspect of 3D culture could be established with similar system of endometrium tissue. We suggest that this study was assumed applicable as baseline data to investigate mechanism between porcine uterus cells in vitro.
        4,000원
        152.
        2013.09 KCI 등재 구독 인증기관 무료, 개인회원 유료
        The endometrium undergoes a cyclic growth and tissue remodeling as changes of epithelial cells, and plasminogen activators (PAs) are related to endometrium tissue remodeling. This study was to evulate expression of urokinase-type plasminogen activator (uPA) and tissue-type plasminogen activator (tPA) in porcine uterine epithelial cells. In results, the uPA and tPA were expressed in uterine tissue, epithelium and secretory glands in porcine endometrial cell. In addition, the uPA and tPA were expressed in cultured epithelial cells, and it were mainly expressed in cytoplasm. In porcine uterine tissue and epithelial cells, uPA activity was higher than activity in tPA. In PAs mRNA expression levels, uPA mRNA level was significantly higher than tPA mRNA level (P<0.05). The fluorescence intensity of uPA protein was also higher than fluorescence intensity of tPA protein, and uPA protein expression was significantly higher than in tPA protein expression (P<0.05). Therefore, we suggest that a physiological function in porcine uterine epithelial cells should be more influenced by uPA than in tPA during pre-ovulatory phase. The endometrium undergoes a cyclic growth and tissue remodeling as changes of epithelial cells, and plasminogen activators (PAs) are related to endometrium tissue remodeling. This study was to evulate expression of urokinase-type plasminogen activator (uPA) and tissue-type plasminogen activator (tPA) in porcine uterine epithelial cells. In results, the uPA and tPA were expressed in uterine tissue, epithelium and secretory glands in porcine endometrial cell. In addition, the uPA and tPA were expressed in cultured epithelial cells, and it were mainly expressed in cytoplasm. In porcine uterine tissue and epithelial cells, uPA activity was higher than activity in tPA. In PAs mRNA expression levels, uPA mRNA level was significantly higher than tPA mRNA level (P<0.05). The fluorescence intensity of uPA protein was also higher than fluorescence intensity of tPA protein, and uPA protein expression was significantly higher than in tPA protein expression (P<0.05). Therefore, we suggest that a physiological function in porcine uterine epithelial cells should be more influenced by uPA than in tPA during pre-ovulatory phase.
        4,000원
        153.
        2013.09 KCI 등재 구독 인증기관 무료, 개인회원 유료
        The present study was performed to investigate the effect of Hh-Ag1.5, a small-molecule chemical agonist of SMOothened receptor, on the in vitro maturation and development of in vitro fertilized (IVF) embryos in pigs. Oocytes or fertilized embryos were cultured in a maturation or embryo culture medium supplemented with 0 (control), 25, 50 or 100 nM of Hh-Ag1.5, respectively. Although the maturation rate were not different among treatment groups, the blastocyst formation rate in the group treated with 25 nM Hh-Ag1.5 was significantly increased compared to other groups (P<0.05). While the highest dose of Hh-Ag1.5 (100 nM) did negatively affect to the embryo development and cell number in blastocysts compared to other groups (P<0.05), the apoptotic cell index in blastocysts was significantly lower in 25 and 50 nM groups than in control and 100 nM groups (P<0.05). The mRNA expression of the proapoptotic gene Bax and the ratio of Bax/Bcl-XL decreased in among treatment groups compared to control (P<0.05). The embryo quality related genes, Tert and Zfp42, were significantly decreased in 50 and 100 nM groups compared with control and 25 nM groups (P<0.05). In conclusion, the addition of 25 nM Hh-Ag1.5 to in vitro maturation and culture medium can enhance the developmental potential as well as quality of IVF embryos in pig.
        4,000원
        154.
        2013.09 구독 인증기관 무료, 개인회원 유료
        An uterus is female reproductive tract organ that affected estrus cycle. During a various changes occur at uterus in estrus cycle, one of them is body fluids secretion be called uterine fluid. Therefore, the objective of this study was to investigate the changes of protein patterns using two-dimensional gel electrophoresis in uterus fluids during the follicular and luteal phases in estrus cycle of pigs. In changes of protein spots were confirmed during the follicular and luteal phases. The 136 spots were expressed in follicular phase, the 57 spots of them showed reproducibility. On the other hand, the 140 spots were expressed in luteal phase, the 73 spots of them showed reproducibility. Also, spots expressed in follicular phase were number 69 and 94 spots and spots expressed in luteal phase only were number 156, 157, 184~187, 190 and 191 spots. The spots which of higher expression levels in the luteal phase than in follicular phase were number 76 and 79 spots. In conclusion, the spots expressed in follicular and luteal phases were confirmed with difference levels and these differences are function of RNA resolving, protein synthesis and cytoskeletal architecture.
        4,000원
        156.
        2013.06 KCI 등재 구독 인증기관 무료, 개인회원 유료
        The objective of this study was to examine the effect of in vitro maturation (IVM) medium, cytochalasin B (CB) treatment during intracytoplasmic sperm injection (ICSI), and electric activation on in vitro development ICSI-derived embryos in pigs. Immature pig oocytes were matured in vitro in medium 199 (M199) or porcine zygote medium (PZM)-3 that were supplemented with porcine follicular fluid, cysteine, pyruvate, EGF, insulin, and hormones for the first 22 h and then further cultured in hormone-free medium for an additional 21~22 h. ICSI embryos were produced by injecting single sperm directly into the cytoplasm of IVM oocytes. The oocytes matured in PZM-3 with 61.6 mM NaCl (low-NaCl PZM-3) tended to decrease (0.05<P<0.1) nuclear maturation when compared with oocytes matured in M199 (76.9% vs. 83.8%) but no significant differences were found in embryo cleavage, blastocyst formation, and mean number of cells in blastocyst (73.8% vs. 74.6%, 11.1% vs. 12.1%, and 28.4 cells vs. 30.1 cells, respectively). The oocyte degeneration was not reduced by CB treatment during ICSI (11.9%) when compared with no treatment control (11.3%) while the treatment showed detrimental effects (P<0.05) on embryonic cleavage (40.0%) and blastocyst formation (1.8%) rates when compared with control (60.0% and 11.5%, respectively). For activation of ICSI oocytes, additional electric stimulus has no positive or negative effect on in vitro development of preimplantation stage ICSI porcine embryos. Our results demonstrate that CB treatment during ICSI inhibits embryonic development of ICSI oocytes and additional electric activation after ICSI has no effect in improving ICSI embryonic development in pigs. Further studies are needed to improve ICSI efficiency by investigating factors influencing embryonic development after ICSI in pigs.
        4,000원
        157.
        2013.06 KCI 등재 구독 인증기관 무료, 개인회원 유료
        The aim of this study is to investigate the genetic interrelationships between economic or meat quality traits (birth weight, weaning weight, average daily gain, market weight, carcass weight, backfat thickness, water, ash, fat, protein, water holding capacity and pH) and 6 SNPs located on six selected candidate genes (MC4R, PGK2, TNNI1, TNNI2, PIK3C3, and CTSK) in Korean native pigs. The genotypes were identified in the 6 SNPs by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) procedure, and association of the genotype on economically important traits was analyzed by general liner model. According to the analyzed results, the MC4R c.1426A>G was correlated with birth weight (p=0.032) and the PGK2 g.122T>G was associated with pH (p=0.026). These findings obtained in the present revealed that the each SNPs of MC4R and PGK2 could be useful as potential genetic markers for birth weight and pH in Korean native pigs, respectively.
        4,000원
        158.
        2013.04 KCI 등재 구독 인증기관 무료, 개인회원 유료
        본 연구는 양돈농가의 사료비 절감을 위해 국내 대표 사료작물인 청보리(유연보리)를 이용하여 양돈용 일반배합사료에 첨가 가능성을 조사하고자 실시하였다. 실험에 사용된 청보리 발효사료는 총체보리를 밀 봉하여 사일리지 형태로 제조하여 보관하였고, 3mm 이하로 분쇄하여 시험사료로 이용하였다. 공시동물은 평균체중 77.71 kg인 3원교잡종 비육돈 72두를 공시하여 돈방 당 6두씩 3반복으로 실시하였다. 처리구는 일반배합사료(대조구)에 청보리 발효사료를 1%, 2% 및 3% 첨가하였고, 42일간 급여하여 종료하였다. 청보리 발효사료 급여는 성장 및 일당증체량에는 영향을 주지 않았지만, 청보리 발효사료 3% 첨가구에서 일일 두당 사료섭취량은 유의적으로 감소하였고(p<0.05), 도체등급에서는 상위등급 출현율이 높았다(p<0.05). 경제성 조사에서 청보리 발효사료 첨가는 일일 두당 사료비를 약 0.55~8.65% 절감하였고, 특히 3% 첨가구에서 사료비 절감 효과가 가장 높았으며, 청보리 발효사료 2% 및 3% 급여구에서 도체가격이 각각 1.53% 및 6.25% 정도 높았다. 따라서 청보리 발효사료 첨가급여는 사료비 절감과 도체등급을 개선하는 것으로 나타났다.
        4,000원
        159.
        2013.03 구독 인증기관 무료, 개인회원 유료
        In order to investigate genetic stability and gene expression profile after cloning procedure, two groups of cloned pigs were used for swine leukocyte antigen (SLA) gene nucleotide alteration and microarray analyses. Each group was consist of cloned pigs derived from same cell line (n=3 and 4, respectively). Six SLA loci were analyzed for cDNA sequences and protein translations. In total, 16 SLA alleles were identified and there were no evidence of SLA nucleotide alteration. All SLA sequences and protein translations were identical among the each pig in the same group. On the other hand, microarray assay was performed for profiling gene expression of the cloned pigs. In total, 43,603 genes were analyzed and 2,150~4,300 reliably hybridized spots on the each chip were selected for further analysis. Even though the cloned pigs in the same group had identical genetic background, 18.6~47.3% of analyzed genes were differentially expressed in between each cloned pigs. Furthermore, on gene clustering analysis, some cloned pigs showed abnormal physiological phenotypes such as inflammation, cancer or cardiomyopathy. We assumed that individual environmental adaption, sociality and rank in the pen might have induced these different phenotypes. In conclusion, the results of the present study indicate that SLA locus genes appear to be stable following SCNT. However, gene expressions and phenotypes between cloned pigs derived from the same cell line were not identical even under the same rearing conditions.
        4,000원
        160.
        2013.02 KCI 등재 구독 인증기관 무료, 개인회원 유료
        본 연구는 돼지의 난포란을 체외성숙하여 세포질내정자주입(ICSI)에 의해 생산된 체외수 정란의 체외발달율을 평가하기 위하여 실시하였다. 세포질내정자주입에 의한 체외수정란의 발달율은 서로 다른 보존상태의 정자인 신선정자, 액상정자 및 동결-융해된 정자를 이용하 더라도 수정율과 배발달율에는 영향을 미치지 않는 것으로 나타났다. 세포질내정자주입 후 난자의 전기적 활성화를 처리한 실험군이 활성화를 처리하지 않은 실험군에 비해 수정율과 배반포기배로의 발달율에 있어서는 높은 경향을 나타내었으나, 체외수정 실험군 및 전기적 활성화를 처리한 실험군과 전기적 활성화를 처리하지 않은 실험군간 배반포기배의 할구수는 유의적인 차이를 나타내지 않았다. 또한 각각의 실험군에서 얻은 배반포기배의 염색체를 분 석한 결과, 정상 이배체 염색체상의 비율에 있어서도 유의성을 나타내지 않았다.
        4,000원