본 연구에서는 항산화 효능과 관능적 특성이 강화된 막걸리 제조를 위해 통단호박을 직접 찐 후 비율별로 첨가하여 막걸리를 제조하였다. 단호박 막걸리 제조를 위해 단호박을 쌀 중량의 5%, 10%, 20% 및 30%를 첨가하였으며 발효 및 담금 후 9일간 단호박 막걸리의 이화학적(pH, 총산 및 알코올), 미생물학적(유산균 및 효모수) 및 항산 화 활성을 조사하였다. pH의 경우 모든 실험군에서 발효 및 저장기간이 늘어날수록 점차 낮아졌으며 9일차에는 단호박 무첨가군인 대조군(3.27)에 비해 단호박 첨가군(3.28- 3.52)이 다소 높은 결과를 보였다. 총산도는 1일차(0.09- 0.55) 및 3일차(0.81-0.86)에 급격히 증가하였으며, 그 후 서서히 증가하여 9일차에는 단호박 첨가군에서 1.16-1.32의 결과를 보였다. 알코올 함량은 9일차에 대조군 0%가 4.59로 가장 낮았고 단호박 5-30% 첨가군에서 5.20-5.77% 로 단호박 첨가량이 많아질수록 알코올 함량이 높았다. 유산균수 측정의 경우 발효초반에 증가하는 양상을 보이다가 5일차부터 다소 감소하거나 유사하여 9일차에는 단호박 첨가 0-30% 첨가군에서 7.95-8.09 log CFU/g로 나타났으며, 효모수의 경우 발효가 진행될수록 증가와 감소를 다소 반복하였으며 9일차에는 7.68-7.99 log CFU/g으로 측정되었다. DPPH 및 ABTS 라디칼 소거능은 단호박 30% 첨가군에서 85.96 및 78.97%로 각각 측정되어 단호박 무첨가군의 DPPH (48.29%) 및 ABTS (62.12%) 결과와 비교 시 높은 항산화 활성도를 보였다. 따라서 추후 단호박 첨가에 따른 각종 비타민류, 무기질 및 β-carotene 등의 영양·기능성 성분의 분석과 더불어 소비자의 관능평가 등을 통해 항산화 기능이 강화된 단호박 막걸리의 실용화를 위한 구체적인 연구개발의 확대가 필요할 것으로 사료된다.

The biosorption characteristics of SRCM 120569 biomass on anionic Congo red (CR) dye. SRCM 120569 strain isolated from Korean turbid rice wine (makgeolli) was identified by 16S rRNA sequence analysis as Bacillus licheniformis. Bacillus licheniformis SRCM 120569 (Genbank Accession No. MW819861) showed superior CR dye biosorption capacity in an aqueous solution. Maximum adsorption capacities of 61.2 and 133.1 mg/g were obtained at pH 3.41 and 0.01 g/50 mL dried cell dosage, respectively. The CR dye adsorption properties by the Bacillus licheniformis SRCM120569 strain were characterized by Fourier transform infrared spectroscopy (FT-IR), point of zero charge (pHpzc) analysis, and phylogenetic analysis. The biosorption isotherm and kinetic models are well described with the Langmuir adsorption isotherm and pseudo-second-order kinetic models.

This study investigated the quality characteristics and antioxidant activities of Makgeolli (a traditional Korean rice wine) made with Etteum bell flower root (0.5, 1, 1.5, and 2% with steamed rice) during fermentation. The pH values of Makgeolli with Etteum bell flower root powder decreased after 3 days of fermentation and then increased after 5 days of fermentation. Sugar content decreased after 3 days of fermentation. Color evaluation showed the L values of these drinks decreased during fermentation, whereas a and b values both increased. The alcohol content of Makgeolli increased after fermentation, reaching a maximum concentration of 7.90-8.07% by the end of fermentation. Total phenolic compound contents and DPPH radical scavenging activities increased as the ratio of Etteum bell flower root increased. Sensory scores of Makgeolli fermented with 1.5% Etteum bell flower root ware greater than those of Makgeolli prepared by other treatments. Therefore, Makgeolli added with 1.5% Etteum bell flower root added considered to be the most suitable for manufacturing.

주박추출물을 첨가한 사과식초의 효율적인 초산발효를 위한 최적조건을 검색하고 그 품질을 조사하였 다. 정상적인 초산균의 생육을 취한 사과식초 사입액의 초기 acetic acid과 ethanol의 농도는 각각 3.0%와 6.0%였다. 사과식초 사입액에서의 초산균의 생육적온은 27℃이였고, 24℃보다 30℃에서 배양 하는 것이 초산생성능이 우수하였다. 초기 주박추출물 농도가 8.0%일 때 초산생성정도가 가장 높게 나 타났다. 초산균은 주박추출물의 첨가농도가 높을수록 유도기가 짧아져 생성되는 초산의 함량이 증가하 다가 8.0%이상의 주박추출물이 첨가되면 유도기가 길어져 생성되는 초산의 함량이 감소하는 것을 확인 하였다. 8.0%의 주박추출물을 첨가하여 발효시킨 사과식초는 대조구에 비해 유도기를 2일정도 단축시 킬 수 있으며 발효 중의 당도변화가 대조구와 거의 차이가 없었다. 유기산 함량을 분석한 결과, acetic acid와 malic acid가 주된 유기산으로 검출되었고 주박추출물을 첨가한 식초에서의 함량은 각각 8,416.9mg%, 103.5mg%로 대조구보다 높게 나타났다. 따라서 8.0% 주박추출물, 3.0% acetic acid, 6.0% ethanol을 첨가한 초기 사과식초에 균을 접종하여 27℃에서 발효하는 것이 발효기간을 단축할 수 있으며 초산과 사과산의 함유량이 높은 식초를 제조할 수 있다.

광펄스 기술은 광범위한 파장의 강한 빛을 짧은 시간동안 조사하여 미생물을 사멸시킴으로서 식품을 살균하는 새로운 비가열 살균 기술로서 주목받고 있다. 본 연구에서는 막걸리를 회분식 방법으로 광펄스 처리하여 살균 효과를 살펴보고, 저장 중의 품질 변화에 대하여 살펴보았다. 광펄스 처리 후 막걸리는 처리하지 않은 막걸리에 비해 2 log cycle 정도의 생균수의 감소를 보였으며, 균수는 1.8-2.7×104 CFU/mL 정도였다. 저장 기간 중 생균수는 4oC에서는 처리구와 무처리구 모두 효모, 세균 및 젖산균의 수가 약간 감소하는 경향을 보였으며, 저장 후 4주간 큰 변화가 없었다. 그러나 30oC에서는 광펄스 처리하지 않은 막걸리의 경우 106정도 수준에서 7일간 일정하게 유지되었으며, 처리구는 2일 정도 후에 무처리구와 비슷한 생균수를 나타내었다. 저장기간 중 품질 변화를 보면 pH는 저장 온도와 상관없이 무처리구와 처리구 모두 약간 감소하는 경향을 보였으나 적정산도는 4oC에서는 일정하게 유지가 되었으나 30oC에서는 무처리구의 경우 2일 후부터 급격한 증가를 보였지만 처리한 시료의 경우에는 저장 6일 후까지 큰 증가를 보이지 않았다. 그리고 환원당의 경우에는 저장 온도에 따라 차이는 있으나 처리구와 무처리구 모두 저장기간동안 지속적으로 감소하였다. 이상의 결과로 보아 막걸리를 적절한 조건으로 광펄스 처리하였을 경우 품질변화를 최소화하고 생균수와 적정산도의 증가를 지연시킬 수 있을 것으로 생각된다.

This study was carried out to investigate the changes in the storage characteristics of the Korean traditional raw rice wine (RRW) treated with Korean sun-dried salts and gamma rays. Nowadays, RRWs have received attention because they are a nutritious foo

This study was conducted to investigate the effects of Takju lees hot water extracts on the blood pressure in spontaneously hypertension rats (SHR). Twenty eight male SHR were grouped by their blood pressure and fed a control diet or experimental diets containing 1% (G1), 2% (G2), or 4% (G4) Takju lees extracts for 4 weeks. Food intake was not significantly different among the groups. However, body weight gain was significantly lower in groups fed the Takju lees extract than the control group. The systolic blood pressure was significantly lower in the Takju lees extract containing groups (especially in G4 group) than the control groups. In addition, mean blood pressure {(systolic+dyastolic)/2} decreased with an increase in the amount of Takju lees extract in the diet and feeding period. Takju lees extract decreased angiotensin I converting enzyme (ACE) activity in a dose-dependent manner. These results suggest that the Takju lees extract exert an antihypertensive effect by decreasing ACE activity.

We investigated the effect of Takju lees extract on blood glucose levels in the db/db mice (a murine model of type 2 diabetes mellitus). We fed 40 male db/db mice a control diet (G0, AIN93G) and experimental diets containing 1% (G1), 2% (G2), or 4% (G4) Takju lees extract for 4 weeks. We found no difference in food intake and body weight gain among the animal groups. In the G1 and G2 groups, plasma glucose levels decreased significantly between Days 10 and 21 compared with the G0 group. However, we found no difference in plasma glucose levels between groups G4 and G0. The change in insulin concentrations was not significant among these animal groups, and we found no significant difference in glucose transporter 4 (GLUT4) expression in the soleus muscle. These results suggest that the Takju lees extract has a beneficial effect in animals with type 2 diabetes.

Takju lees extract is expected to be a promising material for functional food. This study was conducted in order to assess the glycemic index (GI) of Takju lees extract and its effects in an oral glucose tolerance test (OGTT). The GI for Takju lees extract was evaluated with 10 healthy young adults (Male 5, Female 5). OGTT was conducted in 20 male db/db mice, fed on a diet containing 2% Takju lees extract for 4 weeks. Those animals were subjected to OGTT after one oral administration of Takju lees extract at 2 g/kg BW. The GI of the Takju lees extract was measured at 97.97. The effects of the Takju lees extract on the oral glucose tolerance test in db/db mice evidenced no differences as compared to the control group. In conclusion, Takju lees extract is a high GI material, and it has no effect on blood glucose levels in a type II diabetic animal model. Further studies will be required to confirm its anti-diabetic effects.

Effects of Takju(Korean turbid rice wine) lees on noodle investigated by substituting Takju lees water extract powder for each 2% and 4% wheat flour in sample II and III, respectively. The lightness(L) of uncooked noodles was decreased, whereas that of cooked noodles was increased with increasing amount of extract added. Redness(a) and difference of total color(δE) were significantly increased according to the amount of extract added in uncooked and cooked noodles. Yellowness(b) of the noodles containing 2% Takju lees water extract powder was exhibited lowest values in uncooked and cooked noodles. In cooking property of noodles, turbidity of soup decreased in proportion to amount of extract added, 2% and 4% in sample II and III, respectively whereas the weight and volume of noodles were not significant than those of control. Tension of cooked noodle was highest in sample II and lowest in sample III. Texture profile analysis of cooked noodle showed an significant increase of hardness in addition of Takju lees water extract powder but not significant different in springiness, chewiness, adhesiveness. Cohesiveness was significantly lowest in sample II. The sensory evaluation showed that significant difference between noodles with 2% addition groups and control was not recognized but was recognized 4%. Therefore, based on cooking properties and sensory evaluation, Takiu lees water extract powder up to 4% could be substituted for wheat flour.

This study was performed to determine the effect of Takju(Korean turbid rice wine) lees on the serum glucose level in streptozotocin-induced diabetic rats. 24 Sprague-Dawley male rats were divided into three groups: normal control(NC), diabetic control(DC) and diabetic rats(DS) were fed on experimental diet and water ad libitum for 4 weeks. DS diet was containing 20% Takju lees. Body weight gain and food Efficiency Ratio(FER) were significantly lower in DC and DS than NC. DS tended to have higher weight, weight gain and FEF than DC nevertheless food intake. Therefore Takju lees could possibly complement casein as a protein source. Gastrointestianl transit time in DS significantly decreased than NC while not significantly than DC. Serum lipid profiles and AST. ALT and amylase were not significantly different between diabetic DC and DS. Blood glucose was measured at fasting state and 30, 60, 90 and 120 minute by oral glucose tolerance test, DS tended to lower the mean(± SE) incremental blood glucose concentrations than DC and was significantly low at 120 min. But incremental AUG(area under the curve) of postprandial glucose response was not significantly different. In conclusion, in spite of high contents of carbohydrate Takju lees perhaps have a benefit effect on the diabetes.

Recently,mushrooms have received attention because they are material of nutritious food health-stimulating properties and medicinal effects. For the purpose of application the antihypertensive Pholiota adiposa and S. cerevisiae into development of new functional traditional rice wine, optimal fermentation condition and sensory evaluation of functional traditional rice wines were investigated and further its angiotensin-I converting enzyme(ACE) inhibitory activities were determined. Addition of antihypertensive Pholiota adiposa ASI 24012 into the mash was unaffected to ethanol production. The best acceptability showed in the PS-1 traditional rice wines which was brewed byaddition of 0.1% Pholiota adiposa ASI 24012 fruiting body into mash containing antihypertensive S. cerevisiae and cooked and also showed high ACE inhibitory activity of 78.3%.

The antioxidant activities of makgeolli and other alcoholic beverages were compared. Based on the same volume (70 μL eq.) of the alcoholic beverages, the 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid ammonium salt) (ABTS+) radical-scavenging activities were as follows: whisky > makgeolli crude extract (MCE) > rice wine (RW) > clarified makgeolli (CM) > soju. Based on the same alcohol concentration (6%) of the alcoholic beverages, however, makgeolli showed the highest activity. In addition, based on the same volume (70 μL eq.), the inhibition effects against the formation of cholesteryl ester hydroperoxide (CE-OOH) were as follows: soju > whisky > RW > MCE > CM. Based on the same alcohol concentration (6%), however, the inhibition effects against the formation of CE-OOH were as follows: RW > MCE > soju > whisky > CM. Therefore, it was suggested that makgeolli may contain radical-scavenging- and metal-ion-chelate-type antioxidants and may increase the antioxidant activity in the blood.

생산자에게는 상품의 적정가격 보장과 생산의 극대화를 위한 시설 개선으로 소비자의 구매 욕구를 충족시키는 것이다. 막걸리 의 유통 개선 방안을 논의하고 막걸리에 대한 이론과 막걸리 열풍 의 사회적 배경 및 영향, 그리고 막걸리 유통구조의 개선 및 대기 업의 진입으로써 막걸리 업계에 미치는 영향, 지속적인 시장 활성 화 방안에 대해 분석했다. 막걸리 유통구조와 문제점은 현재까지 많은 문제점과 개선방안 이 실재하였음에도 불구하고, 개선책이 마련되지 않은 이유는 소 비자에 대한 부정확한 문제 인식이 주요원인이며, 막걸리 유통구 조 개선의 궁극적인 목적은 생산자에게는 막걸리 시장의 활성화로 인한 생산 시설의 개선이며, 소비자에게는 적정한 가격에 안전하 고 좋은 품질의 상품을 공급받는 것이다. 위에서 언급한 바와 같 이 물류시스템의 개선과 위생안전 관리를 위한 정부의 지원 등을 통한 변화가 필요하며, 이에 따르는 많은 막걸리 업체들이 유통의 주요 문제로 인식하여야 할 것이다.