Saengcheonggukjang, known as Natto in Japan, is a soybean fermented food which is made from steamed soybean, Bacillus and water. Demand of Saengcheonggukjang has increased because it does not have much smell compared to Cheonggukjang. Seven varieties of Saengcheonggukjang were investigated and compared in terms of 100 seed weight, quality characteristics, hard seed rate, and water absorption rate in order to determine the suitability of Korean soybeans. In addition, each characteristic of Saengcheonggukjang was compared. ‘Hoseo’ and ‘Haewon’ showed low 100 seed weight with 8.41 g and 8.11 g, respectively. The water absorption rate was higher in ‘Hoseo’ and ‘Pungwon’ than in Japan varieties. The yield of Saengcheonggukjang was significantly different for each variety. No differences were observed in yield and hardness of Saengcheonggukjang with respect to the varieties and sowing date. ‘Haewon’ showed the highest amino nitrogen content with 575.0 mg%. Viscous material content did not appear to differ between varieties. These results suggested that ‘Hoseo’ and ‘Haewon’ can be considered as suitable candidates for yield and quality of Saengcheonggukjang compared to Japan varieties.

This experiment was conducted to evaluate feed value and usability of soybean varieties as livestock forage. In this study, three soybean cultivars, OT93-26, Geomjeongsaeol, and Pungwon, were harvested at R5 (beginning seed development)- and R6 (full seed)-reproductive stages for analyzing feed value of soybean. Days to R5 stage harvest of OT93-26 among the three soybean cultivars was 55 days and the shortest while Pungwon took 103 days to reach at R6 stage. The R6-harvested soybeans had higher dry matter (DM) yields and crude protein (CP) content than the R5-harvested. However, both DM and CP were the highest in the R6-harvested Geomjeongsaeol. Contents of neutral detergent fiber (nDF) and acid detergent fiber (ADF) of Pungwon harvested at R5 were the highest whereas the R6-harvested Geomjeongsaeol had the lowest. Digestible dry matter (DDM), dry matter intake (DMI), and relative feed value (RFV) of the R6-harvested Geomjeongsaeol and Pungwon were higher than those of the R5-harvested, but in case of OT93-26, those at R6 stage were low rather than those at R5 stage. However, soybean could be used as alternative forage with high feed value for livestock. Taken together, Geomjeongsaeol could be used for developing new forage soybean varieties with high feed value, and R6 would be the optimum harvesting stage for yield and quality of forage soybean.

This study was performed to select the high-yield soybean cultivar suitable for the mid part of Korea and to provide as a basic data for improving productivity of the farmhouse. To this, Daewon as a control and four soybean cultivars such as Uram, Taeseon, Saegeum and Seonpung soybean seeds known that high-yield varieties developed after 2010 were selected. Growth characteristics and functional isoflavone contents of five soybeans were field tested at the field in Goesan, Chungbuk during 2014 and 2015. In comparison with Daewon, maturity date was recorded the earliest at Taesun with 15th Oct. Height of the first pod for the mechanical harvesting have a benefit to Uram (16.8 cm) and Taeseon (14.6 cm), however; shattering percentage of Taeseon were showed the high value of 11.2%. Productivity of Uram, Seonpung and Taesun were increased with 20, 9, and 5 %, respectively, compare to that of Daewon. Total isoflavones contents (μg/g) of five soybeans were ordered as Saegeum (1222.2) > Uram (1111.9) > Seonpung (897.2) > Daewon (885.8) > Taesun (829.5). Total isoflavones contents when soil mulching compare to the non-mulching were increased 206.6 μg/g at Daewon and 460.8 μg/g at Uram soybean.

미이라병은 Diaporthe/Phomopsis complex에 의해 유발되는병으로 콩 재배기간 중 따뜻하고 습한 환경에서 종자가 성숙되면 감염률이 높아지며 감염된 콩 종자는 외관상 품질뿐만아니라 종자 활력이 저하된다. 미이라병에 대한 연구를 수행하기 위하여 대만에 위치한 아시아채소개발연구센터(AVRDC)의 콩 시험포장에서 미이라병 병징을 보이는 콩 줄기를 채집하고 이로부터 3개의 곰팡이 균주(isolate)를 분리하였다. 배지위에서의 곰팡이 균사의 생육특성, 현미경하에서 관찰된 알파,베타 분생자(conidia)의 모양 그리고 PCR-RFLP 분석으로, 3개의 균주는 Diaporthe phaseolorum var. sojae 으로 확인되었다. 한편, 미이라병 저항성 육종을 위해서는 유전자원과 계통의 검정이 선행되어야 하는데, 인공접종을 위해서 분생자의최적 배양조건을 탐색하였다. 그 결과 배지는 PDA, 온도는24oC에서 잘 배양되었으며, 일장은 암조건에서는 균사체만 유도되고 분생자는 유도되지 않았으며, 24시간과 15시간의 일장에서는 균사체 유도 및 분생자의 유도 정도에 차이가 없었다.또한 잎-줄기와 꼬투리, 두 개의 접종 부위에 따른 미이라병감염률을 조사하였는데, 두 접종 부위에 따른 미이라병 감염정도는 통계적인 유의차는 나타나지 않았으나 잎-줄기에 접종한 개체 보다 꼬투리에 접종한 개체의 종자 감염률이 높은 경향을 보였다.

A new soybean variety, ‘Joongmo 3009’ (Milyang 222) was developed at the National Institute of Crop Science (NICS) in 2012. ‘Joongmo 3009’ was released by pedigree selection from the cross between ‘Cheongja 2(Milyang 121)’ and ‘Daemangkong’. It has determinate growth habit, white flower, brown pubescence, brown pod color, green seed coat, green cotyledon, spherical seed shape, oval leaf shape and large seed size (29.3 grams per 100 seeds). It was late 16 days in maturing date than the check cultivar ‘Cheongjakong’. The average yield of ‘Joongmo 3009’ was 2.91 ton per hectare, which was higher 36 percentage than the check variety, in the regional yield trials carried out in three adaptable locations of Korea from 2010 to 2012. The number of breeder’s right is ‘5474’

Thirty-eight Pea (Pisum sativum L.) genotypes were screened to identify varieties to be suitable for sprout. Based on seed yield and sprout qualities such as whole length and sprout yield, five genotypes (PI269803, PI343278, PI343283, PI343300 and PI 343307) were primarily selected as candidates for pea sprouts. In order to determine optimal cultivation condition for pea sprouting, growth characteristics were investigated according to the change of germination temperature and days for sprouting. Whole length and hypocotyl length were observed to increase as a time dependent manner at each tested temperature (20, 23, and 25°C). However, whole length, hypocotyl length, and sprout yield were highly increased at 23°C compared to 20 and 25°C. Especially, PI269803 and PI343300 showed higher sprout yield than the others. In addition, the effect of the change of germination temperature on antioxidant properties was estimated by measuring total phenolic content (TPC) and free radical scavenging activity (DPPH and ABST activity). TPC and DPPH/ABST activities of PI269803 and PI343300 were higher at 23°C than at 20 and 25°C, while antioxidant properties of PI343278 and PI343283 were decreased in a temperaturedependent manner. The results show a high degree of correlation between TPC and antioxidant activities and suggest that the temperature change for pea sprouting could be responsible for antioxidant properties. Taken together, these results provide optimal cultivation conditions for pea sprouting and suggest that PI269803 and PI343300 with high sprout yield and antioxidant properties could be used for pea sprouts.

‘연풍’은 1999년에 대립이며 황색 종피이며 다수성인 ‘수 원191호’를 모본으로 하고 경장이 짧고 조생종인‘수원196호’ 를 부본으로 교배되었다. 2000년도에 F1을 양성하고, 2001년 도에 F2는 집단선발을, 2002년 ~ 2004년도에 F3 ~ F5세대를 계통육종법으로 선발한 HS982-B-1-1-2-2 계통으로 계통명은 ‘연천3호’이다. ‘연풍’의 주요특성을 요약하면 다음과 같다. 신육형은 유한신육형이며 꽃색은 자색이고, 엽형은 피침형 과 능형의 중간형이며, 모용색은 회색이고, 종실모양은 편구 형이며, 제색과 종피색은 황색이고 협색은 갈색이고 협은 성 숙 후에도 잘 개열되지 않으며, 성숙기는 대원콩보다 15일 빠 른 조중생종이며, 100립중은 28.7 g으로 대립이다. SMV유묘 검정에서는 하위엽에서 모자이크증상을 보였으나, 포장에서 는 0이었다. 검은뿌리썩음병의 발병율은 2.5%였다. 불마름병 유묘 검정에서는 5 정도였으나, 포장에서는 0이었다. 콩나방 은 1.5%였다. 종실의 화학적 성분은 단백질은 36.1%이었는 데 특히 아미노산 함량은 346.8 ㎎/100g, 아이소플라본 함량 은 1,667 ㎍/g으로 대원콩보다 낮았다. 조지방은 19.2%였는 데, 대원콩보다 포화 지방산은 11.8%로 1.6% 높았으나, 불포 화지방산은 85.0%로 1.2% 낮았다. 가공적성에서 두부수률은 대원콩과 비슷한 수준이었으나 두부물리성 중 경도, 검성, 응집성은 비슷한 수준이었으나 경도는 낮았다. 청국장의 발효 및 풍취는 대원콩보다 우수하였다. 수량성은 대비품종인 대원콩보다 생산력검시험에서는 16% 증 수 되었고, 3개년 간 실시한 지역적응시험에서 3.44 MT/ha으 로 38% 증가하였다(품종보호번호: 제4242호).

Soybean mosaic virus (SMV) is a prevalent pathogen that causes significant yield reduction in soybean production worldwide. SMV belongs to potyvirus and causes typical symptoms such as mild mosaic, mosaic and lethal necrosis. SMV is seed-borne and also transmitted by aphid. Eleven SMV strains, G1 to G7, G5H, G6H, G7H, and G7a were reported in soybean varieties in Korea. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) method allowed one-step detection of gene amplification by simple procedure and needed only a simple incubator for isothermal template. This RT-LAMP method allowed direct detection of RNA from virus-infected plants without thermal cycling and gel electrophoresis. In this study, we designed RT-LAMP primers named SML-F3/B3/FIP/BIP from coat protein gene sequence of SMV. After the reaction of RT-LAMP, products were identified by electrophoresis and with the detective fluorescent dye, SYBR Green I. under daylight and UV light. Opmtimal reaction condition was at 58℃ for 60min and the primers of RT-LAMP showed the specificity for nine SMV strains tested in this study.

Generally, the virus was detected by the ELISA using the serological method and RT-PCR based on the genetic information. Recently, NGS (next-generation nucleotide sequencing) has been used in genome analysis and diseases diagnostics. To identify distribution aspects of viruses, we collected diseased samples twice in soybean breeding field. After extraction of total RNA from the collected bulk samples, RNA was sequenced by the NGS method. The NGS data were analyzed using the bioinformatics software. With newly produced NGS data, the identification of distribution aspects of organisms in field was estimated in this study. Sequence based identification method should be more accurate diagnostic tools of the target diseases and be able to predict occurrence of potential and new pathogens. NGS method will also provide the basic data by identifying the distribution of using bacteria. In this study, we analyzed the extracted RNA from the collection of approximately 3000 samples. Consequently, we confirmed the following types: the 7,089 kinds of bacteria including Burkholderiaceae, the 13,397 kinds of Eukaryota, the 952 kinds of viruses from the first bulk samples, the 4,160 kinds of bacteria including Burkholderiaceae, the 10,475 kinds of Eukaryota, and the 576 kinds of viruses from the second bulk samples

‘만풍’은 1998년에 두부 특성이 우수한 동산 128호를 모본으로 하고 미국에서 도입된 Sprite 87을 부본으로 인공교배하였다. 1999년에 F1을 양성하고, 2000년에 F2는 집단선발을, 2001년부터 2002년까지 2년에 걸쳐 AVRDC에서 SSD (single seed descent)로 F3-F5세대를, 2002년도에는 F6세대를 계통육종법으로 선발한 YS1325-B-B-S-S-S-100계통으로 계통명은 ‘연천 1호’이다. ‘만풍’의 주요특성을 요약하면 다음과 같다. 신육형은 유한 신육형이며 꽃색은 백색이고, 엽형은 난형이며, 모용색은 회백색이고, 종실 모양은 편구형이며, 제색과 종피색은 황색이고 협색은 담갈색이고 협은 성숙 후에도 잘 개열되지 않으며, 성숙기는 태광콩보다 9일 빠른 중생종이며, 100립중은 29.5 g으로 대립이다. 병 저항성은 뿌리썩음병은 강하고, 불마름병은 1이었고 콩나방은 1.5%였다. 종실의 화학적 성분은 단백질은 41.3%, 조지방은 19.3%였고, 특히 아미노산 함량은 381.2 mg/100g, 아이소플라본함량은 2,425 ㎍/g으로 태광콩보다 높았다. 가공적성은 태광콩보다 두부 수율은 230%로 높았고, 경도는 낮아 부드러웠고, 청국장의 발효 및 풍취는 우수하였다. 수량성은 대비품종인 태광콩보다 생산력검시험에서는 14% 증수 되었고, 3개년 간 실시한 지역적응시험에서 단작에서는 2.41 MT/ha으로 3% 낮았으나, 이모작 재배 시에는 2.87 MT/ha으로 12% 증수하였다.

‘청아’는 내재해 다수성 장류용콩 신품종을 육성하기 위하 여 내도복, 다수성 품종인 ‘신팔당콩 2호’를 모본으로 1999년 도에 인공교배한 SS99505 조합으로 계통육성법으로 선발하였으며 계통명은 ‘강원 110호’이다. 꽃색은 백색이고 잎모양 은 난형이며, 종자모양은 구형이다. 종피색과 배꼽색은 황색 이고 백립중은 25.2 g으로 ‘태광’보다 2.4 g 무거운 대립이다. ‘태광’에 비하여 개화기는 7월 20일로 11일 빠르고 성숙기는 9월 30일로 5일 빨라 조숙종이면서도 등숙기간은 6일 길다. 특히, 도복에 강하며 불마름병과 바이러스에 강하다. 수량성 은 ‘태광’보다 생산력검정시험에서는 15% 증수하였고, 철원 등 4개 단작지역 적응성검정시험에서는 14% 증수하여 평균 종실수량이 ha당 2.72톤이다.

‘원흑’은 나물콩 특성이 우수한 고품질 내재해 다수성 신품종육성을 목표로 ‘다원콩’과 ‘진주1호’의 F1에 ‘진주1호’를 다시 교배하여 계통육종법으로 선발한 YS1318B-2B-3-3-3-4-1계통으로 계통명은 익산63호이다.유한신육형으로 꽃색은 자색이고 잎의 모양은 난형이며 종피색과 제색은 흑색이며, 성숙기가 지나도 협개열에 강하다. 성숙기는 10월 4일로 ‘다원콩’보다 10일이 늦은 만숙종으로 경장이 다소 길고, 꼬투리수가 많으며, 종자 100립의 무게는 8.8 g으로 ‘다원콩’보다 작은 소립이다. 바이러스 접종시 모자이크를 보이나 포장에서는 강하며 불마름병은 ‘다원콩’보다 강한 중도저항성을 보였다. 종실의 조단백과 조지방함량은 ‘다원콩’과 비슷하며, 콩나물의 아스파라긴산 함량은 낮았으나 이소플라본 함량이 ‘다원콩’의 2배 이고, GABA 함량이 높은 고기능성 품종이다. 콩나물재배시 경실과 비정상발아율이 낮고 발아율과 배축신장성이 좋으며 수율이 풍산나물콩에 비해 20% 높았다. 수량성은 2007년부터 2009년까지 3년간 실시한 지역적응시험 결과 전국 6개소 평균수량은 ha당 2.34톤으로서 ‘다원콩’보다 128% 증수하였다.

국내 콩에서 발생하는 세균병해인 불마름병, 들불병, 세균점무늬병, 세균갈색점무늬병의 다중 진단을 위한 PCR 방법을 요약하면 다음과 같다.1. 콩에 발생하는 각각의 세균들은 서로 다른 박테리오신(bacteriocin) 이나 파이토톡신(phytotoxin)을 생산하는데 이와 관련한 유전자를 목적으로 하여 진단프라이머를 설계하였다.2. 불마름병은 glycinecin A, 들불병은 tabtoxin, 세균점무늬병은 coronatine과 세균갈색점무늬병은 syringopeptin을 목적유전자로 하여 다중 진단프라이머 조합을 설계하였다.3. 1차 선발로 각각의 균주에 대한 단일 진단 프라이머를선발하였으며, 여기선 선발된 21개의 프라이머들을 조합하여 4종 다중진단프라이머 선발을 위한 2차 선발에 이용하였다. 최종적으로 280 bp의 불마름병, 355bp의 세균갈색점무늬병, 563 bp의 들불병과 815 bp의세균점무늬병으로 구성된 다중진단 프라이머 조합이개발되었다.4. 선발된 4종 다중 진단 프라이머 조합의 경우 다른 세균들과의 비특이적 반응이 있는지 확인하기 위한 3차선발을 거쳐 그 특이성을 검증하였다.

‘대하 1호’는 대립 내재해 다수성 콩 신품종육성을 목표로, 내재해 다수성 ‘장엽콩’을 모본으로 대립 ‘화엄풋콩’을 부본으로 하여 교배한 것을 다시 다수성 ‘수원192호’를 모본으로 1997년도에 인공교배한 SS97214 조합으로 계통육성법으로 선발하였으며 계통명은 ‘밀양164호’이다. 꽃색은 백색이고 잎모양은 난형이며, 종자모양은 구형이고 종피색과 배꼽색은 황색이며 100립중은 25.4 g으로 ‘태광콩’에 비하여 2.5 g 정도 무거운 대립이다. ‘태광콩’보다 성숙기가 늦어 등숙기간이 2일이 길다. 도복에 강하며, 불마름병과 바이러스에 강하고, 내습성과 뿌리썩음병, 종실의 이병립율은 ‘태광콩’과 비슷하다. ‘태광콩’ 대비 수량성은 생산력검정시험에서 8 %, 지역적응시험에서는 5 % 증수하였으며, 전국 10개소 평균 종실수량은 ha당 2.62톤이다.

‘천상’은 대립 다수성 콩 신품종육성을 목표로, ‘수원190호’를 모본으로 ‘황금콩’을 부본으로 1998년에 인공교배하여, 1999-2000년도에 F1, F2 세대를 양성하고, F3부터 계통 전개하여 선발한 SS98206-41-1-3-1-1 계통으로 계통명은 ‘밀양176호’이다. 주요 특성은 유한신육형으로 화색은 백색이고, 엽형은 피침형이며, 모용색은 회색이다. 종자모양은 구형이고, 종피색과 제색은 황색이다. 개화기는 7월 26일로 ‘태광콩’보다 빠르고 성숙기는 3일 늦어 등숙기간이 7일이 더 길다. 경장은 76 cm로서 ‘태광콩’과 비슷하고 도복에 강하며, 100립중은 24.8 g으로 ‘태광콩’에 비하여 2.0 g 정도 무거운 대립이다. 불마름병에 저항성이며 콩모자이크바이러스와 내한발성은 강하였으나, 내습성과 뿌리썩음병에는 약하였으며, 탈립이 되지 않았다. 조단백질 및 조지방 함량은 ‘태광콩’보다 높았으며, 불포화지방산 비율은 ‘태광콩’과 비슷하였고 함황아미노산과 총 아미노산의 함량이 높았다. 두부수율은 낮으나 두부의 물성이 높았으며, 메주 가공적성은 ‘태광콩’과 비슷하였다. 수량성은 생산력검정시험 결과, 10% 증수되었으며, 지역적응시험에서는, 전국 10개소 평균 ha당 2.34톤으로서 대비품종인 ‘태광콩’ 대비 100%이었다.

‘대흑’은 밥밑용 특성이 우수한 단경 내재해 다수성 신품종 육성을 목표로 대립 장류용 품종인 대황콩과 단경 내도복 내재해 다수성 계통인 밀양 79호를 1997년 인공교배하여 계통 육종법으로 선발한 YS1298-2B-20-5-1-1-1 계통이다. 계통명은 ‘밀양158호’이고 품종등록번호는 No. 3009이며 주요특성은 다음과 같다. 1. ‘대흑’의 신육형은 유한신육형이며 화색은 자색이고 엽형은 환형이다. 모용 및 성숙기의 협은 갈색이며 종피색과 제색은 흑

We previously reported that reverse transcription-polymerase chain reaction/restriction fragment length polymorphism (RT- PCR/RFLP) was an effective method to identify SMV strains. Using this method a new SMV strain G6H was successfully identified. To introduce resistance locus Rsv4 of V94-5152, we made crosses between two parents, Hwanggumkong and V94-5152, and obtained 6 BC3 F3 progeny lines, which have different size of DNA fragment of Rsv4 locus region. To confirm the virus resistance of progeny lines, artificial inoculation were conducted with 10 SMV strains, G1-G7, G7A, G6H, and G7H. Genomic DNA of tested lines was extracted and used marker genotyping using 9 SSR marker, which covered about 20 cM genetic distance including Rsv4 locus. In the virulence test, only two progeny lines showed resistance to all the SMV strains like a V94-5152. However, the other lines showed necrotic symptoms to G6H strain. It is considered that a minor gene is located near the Rsv4 locus between Satt157 and Sat_254 marker which interacts with G6H. A new strain can be a clue to find a minor gene in the SMV resistance soybean breeding.

Soybean (Glycine max(L.) Merr.) is an important source of high protein and oil. Use of soybean meal bythe food industry is increasing, but severely limiting dietary choices and the quality of life of food-allergic individuals. Gly m Bd 30K (P34) is known as the main seed allergens in soybean-sensitive patients. The objective of this work was to determine the molecular basis of the low mutation of soybean P34 and to design polyclonal antibody for the selection of the causative mutations for wild homozygous, heterozygous and mutant homozygous. Using soybean genome assembly, we knew that soybean P34 genes are existing 2 copies in LG A1 and 1 copy in LG A2 in soybean genome. Actually, we confirmed that 3 copies for P34 gene were existed on soybean genome with Southern blot analysis. Glyma08g12270 of those was expressed at significantly higher level compared with Glyma08g12280 and Glyma05g29130by RT-PCR and western analysis. However this gene was not expressed in the low-P34 germplasm accessions. We develop a co-dominant marker based on the sequence of Glyma08g12270 containing a four-base pair insertion at the P34 start codon. Also, we make a polyclonal antibody for investigation of P34 protein levels. Further study, we will perform the crossing between low P34 accession and elite variety, backcrossing and allergen test using low P34 line.

Soybeans X soybeans mosaic virus (SMV) strains interactions affected plant growth and seed transmission. Strain virulence of SMV depended on host cultivars. Kwangankong and Tawonkong were susceptible to G7H and G5 strains, causing mosaic symptoms. The distribution patterns of two SMV strains in soybean plants inoculated with G7H, G5 and G7H/G5 sets were investigated by RT-PCR/RFLP analysis. In the first treatment, two primary leaves in a single plant were infected with both strains by means of one strain per leaf. The leaves of Kwangankong and Tawonkong at V2, V4 and V6 stage were doubly infected with the two strains and the upper leaves than those had only G7H strain. Secondly, the two soybeans were inoculated with G7H, and 24 h after followed by the other strain inoculation. The leaves of V6 and V8 stages in all infected plants showed mosaic symptoms caused by G7H, and there was no detection of G5 strain. In contrast, the reverse treatment with G5 and G7H induced different results. Pre-inoculated G5 strain detected in every stage besides G7H strain. Host X SMV strain compatibility influenced seed coat mottling, yield, plant height, number of pod per plant. G7H had a seed mottling rate of 98.5% in Kwangankong, while G5 had an incidence of seed mottling of 1.4% in the same cultivar. G5 was more virulent to Kwangankong and had a lower affinity for infecting soybean seed mottling. Additional inoculation of G7H protected soybean yield and growth from G5-inducing loss in Kwangankong.

Colored soybean for cooking with rice have been used traditionally in Korea which is distinguished from other countries. Although many soybean cultivars have been developed for cooking with rice since the launch of the first cultivar Geomgeongkong1 in 1994, the breeding history of soybeans for cooking with rice is not quite long comparing to that of soybean paste/ bean curd and soy-sprout. In addition to developed cultivar, various landrace soybeans have still used for cooking with rice to Korean. This study was performed to select useful breeding materials and to evaluate the diversity of Korean landrace germplasms, especially black and/or green color seed coat soybeans. About five hundred eighty Korean colored soybean landraces were investigated for agricultural traits in experimental field and for DNA diversity using five SSR markers which showed high polymorphism between Korean soybean cultivars in a previous study. PowerCore (v. 1.0) software (http://genebank.rda.go.kr/PowerCore/) was used to analyze diversity of our landraces and to construct core set. In conclusion, we could obtain core set of forty-five germplasms by PowerCore analysis. Satt002 in analysed five SSR markers had twenty-two alleles and well represented diversity of black and/or green color germplasms.