친환경 벼 재배농가에서 혼합유박비료를 관행적으로 이앙 3일 전에 사용함에 따라 분얼 기 이후에 비료효과가 나타나므로 수량이 감소하는 문제를 해결하기 위해 본 연구를 수행 하였다. 실내에서 조사한 혼합유박비료의 무기화는 담수조건에서 7주까지 NH4-N가 증가하 였고, 비담수 조건에서 5주까지 NO3-N가 증가하였다. 벼 이앙 20일에 논토양의 질소무기화 는 이앙 20일 전 처리구가 0.85%로 가장 높았다. 수확기 토양특성은 혼합유박비료 시비시 기에 따라 토양유기물함량을 제외하고 차이가 없었다. 이앙 60일에 초장, 간장, 수장은 이 앙 20일 전 처리구가 가장 길었다. 또한 이앙 20일 전 처리구에서 수수, 등숙비율, 천립중이 높게 나타나 백미수량이 관행처리(이앙 3일 전)에 비해 9% 증가하였다. 백미의 백도와 amylose는 차이가 없었지만, 단백질 함량과 식미치는 이앙 20일과 30일전 처리에서 같은 수 준이었다. 쌀 수량과 품질 등을 혼합유박비료 시용시기와 관계식으로 산출하면 혼합유박비 료의 적정 사용 시기는 이앙 21.3일 전으로 나타났다.

Background : Although ginseng has various bioactive compounds in it, there is lack of study on the variations of bioactive compounds in ginseng according to the cultivation soil and the applied fertilizer types (or amount). Therefore, this study aims to examine the variations of 37 fatty acids (FA) and 8 vitamin E (Vit-E) vitamers in 6-year-old ginseng root cultivated in different soil types with different fertilizers regimes. Methods and Results : The profiling of 37 FAs and 8 Vit-E vitamers in 6-year-old ginseng roots was measured by gas chromatography coupled with a flame ionization detector, and then these results were statistically analyzed with chemometrics. The FA and Vit-E content in ginseng roots varied significantly with respect to soil cultivation conditions due to organic fertilizer types and amounts used. Unsaturated FA in ginseng is approximately 2.7 fold higher than the saturated FA. Linoleic, palmitic, and oleic acids were the most abundant FAs found in the ginseng roots. Also, the major Vit-E vitamer found in ginseng root is α-tocopherol. In particular, the application of rice straw compost or food waste fertilizer was increased to create nutritionally desirable FAs and bioactive Vit-E in ginseng root. In addition, phytonutrient profiling coupled with chemometrics can be used to discriminate the cultivation conditions of ginseng. Conclusion : This study extends our understanding about the variations of FA and Vit-E in ginseng root depending on cultivation conditions. Hence, these results can be useful as basic information for reliable ginseng production containing high amounts of phytonutrients in a paddy-converted field.

Background : This study aimed to determine the use of proper organic fertilizer through side dressing application in the environmental cultivation of Adenophora triphylla var. japonica Hara. to improve farming income. Methods and Results : In the cultivation of eco-friendly and environmental medicinal plant like Adenophora triphylla var. japonica Hara., it is necessary to use organic instead of chemical fertilizer. Organic fertilizers such as HL, CK, OK were applied three (3) times during the last ten days of July, August, and September. There was no significant difference between CF and NT, in terms of the number of rooting per 1㎡ which was 12.1 -12.9. There were also no significant differences in plant length, leaf length, leaf width and dry weight. The root length was 19.9 ㎝, which was the longest in CK, and 16.8 ㎝, the shortest in ST, but described as no significant difference. The root width was 14.8 – 5.5 ㎜, and the number of rootlet was 5.7 - 7.1, but was considered not a significant difference. The dry weight was 16.6 g in HL, 16.3 g in CK and ST, and 15.6 g in OK, but still there were no significant differences observed. However, it was 14.6 g in NT, which was considered the lowest, thus there was a significant difference. The number of dry root per 10 a was 149 ㎏ which is considered the most in HL, 141 ㎏ in CK, 140 ㎏ in ST, and 138kg in OK yet there were no significant differences observed. While NT only had 123 ㎏ hence, there was a significant difference on the number of dry root per 10a as compared with the group applied with the three organic fertilizers. Conclusion : In conclusion, it is proper to use Organic Fertilizer HL and CK through side dressing application in growing Adenophora triphylla var. japonica Hara.

This experiment was conducted to assess changes to the chemical properties of soil and applicability in a case of rice cultivation with organic fertilizers. The investigator applied organic fertilizers to rice cultivation for five years to examine changes to the chemical properties of soil and found that the experiment group of organic fertilizers made an ongoing increase in pH, organism content, and available phosphate content annually compared with the control group with no big differences according to the amounts of organic fertilizers used. As for the yield components, there were no statistical differences in the number of spikelets and grain filling rate between the experiment group of organic fertilizers and the control group. The experiment group recorded a higher level in 1,000 seeds weight and yield than the control group. Experiment Group 4 recorded the highest level at 29.11 kg of 1,000 seeds weight. Experiment Groups 3 and 4, which used 222 kg and 267 kg per 10 a, respectively, recorded 576 kg and 572 kg of yield, respectively, which were 4.7% and 4.1% higher than 549 kg of control group, respectively. As for the quality of brown rice, there were no statistical differences in the head rice percentage between the control and experiment group, both of which were in the range of 83.2-85.7%. As for the protein content, Experiment Groups 3 and 4, both of which used a lot of organic fertilizers, were in the range of 6.9-7.1%, which was lower than 7.5% of control group. Those findings indicate that the long-term application of organic fertilizers can improve the chemical properties of soil and increase the yield more than the conventional method of fertilizer application. The findings also suggest that it will be effective to apply 222 kg of organic fertilizers or more per 10 a.

Background: The roots of Atractylodes macrocephala Koidzumi contain atractylone, which is used to suppress appetite and indigestion caused by gastrointestinal disturbance. The present study was conducted to investigate the effect of several organic compost on the growth and root yield of A. macrocephala with organic fertilizer.Methods and Results:When organic fertilizer was applied basally, the average yield of 10 a was 184.6 ㎏ in the HA (Hwanggeumjidae, organic material mix), 171.3 ㎏ in the GG (Gyunbaeyangchegreen, bacterial cuture filtrate) and 175.0 ㎏ in the CF (Customary fertilization, control) each other in practice of CF had no statistical significance. Atractylenolide I was significantly greater in the HA (0.036%) than the GG (0.034%) or CF (0.023%). With regard to the amount of organic fertilzer, 10 a yield ws the most common of 203.0 ㎏ at 2.0 times of the organic 1 (HA), conventional fertilization of 134.0 ㎏ and 173.0 ㎏ of no application was a statistically significant. Organic fertilizer 1 was 1.5 to 2.0 times, organic fertilizer was 2 to 1.5 times that were most suitable.Conclusions:The results of the present study indicated that HA and GG are the most suitable for the organic cultivation of A. macrocephala. The content of atractylone I was highest under the HA treatment and lowest under the CU (Chamjoa, oil cake), TG (Totogreen, plant oil cake) and HG (Heuksalgreen, Castor oil cake) treatment.

Background : This study was investigated the effect of organic fertilizer application and stem training methods on the growth and yield of Cynanchum willfordii. Methods and Results : Traditional species, C. willfordii conducted the test to the field in Chungbuk ARES, Korea. Two methods, non-supporting (custom) and I-shape supporting was used for stem training method. Planting spacing was adjusted to 30 (interrow spacing) x 20 cm (intrarow spacing) and other key management is the followed the cultivation manual for standard medicinal crops. Organic fertilizer application is conducted in mid-March before planting of C. willfordii. Registered organic agricultural materials such as fungal cultures (CC), mixed organic materials (MO), fermented and mixed expeller cake (EC), and fermented fowl dropping (FD) was used. Application rate is based on the nitrogen application rate after soil testing. Plant height was both good in all at I-shape supporting (266.7 ㎝) and all in non-supporting (160.8 ㎝); however, stem diameter was more thicker in custom (4.6 ㎜) of I-shape supporting and EC (4.8 mm) of non-supporting. Number of branched stem were good at EC from I-shape supporting and non-supporting, as well as leaf growth. SPAD value was evaluated in MO (52.7) of I-shape supporting and EC (52.3) of non-supporting. Number of branched root per organic fertilizer were showed 7.0 at MO from I-shape supporting, but 7.3 at FD from non-supporting. While length of main root were 27.0 cm in MO of I-shape supporting and 31.3 ㎝ in FD of non-supporting. Root weight also more heavier in EC (66.3 g) of I-shape supporting and FD (53.0 g) of non-supporting. When applied organic fertilizer, total density of soil microorganisms were changed into 38.4 ppm in custom; however, it more plentiful of 90.7 ppm in MO, it showed good effect on the replication of soil microbiota. Conclusion : From the investigated results, MO of I-shape supporting was good at overall growth, including good tendency of roots growth.

This study was conducted to elucidate the effect of organic fertilizer on growth characteristics, saponin contents and antioxident activity of Platycodin grandiflorum Ridix roots for organic farming. As basal fertilizers, chemical fertilizer, mixed organic fertilizer, bacterial cultures and fermented oil cake and decomposed manure were treated based on 3 ㎏/10a level before transplanting Platycodin grandiflorum Ridix. In root length, when chemical fertilizer plot and mixed organic fertilizer plot were treated, root length was recorded the highest scores (25.3 and 24.0 ㎝) Root width was recorded the highest score (26.6 ㎝) in chemical fertilizer plot. The number of fine-roots was the highest in mixed organic fertilizer plot and chemical fertilizer plot (20.0 and 17.0), respectively. Fresh weight, which affects directly yield, was the highest in organic fertilizer plot (55.7 g/plant). The content of Platycodin D was shown to 327.4~373.8 ㎎/100 g, the highest values were observed in organic fertilizer plot. The total polyphenol and flavonoid contents were recorded the highest scores (15.5 and 15.3 mg/g, respectively) in organic fertilizer plot. In this study we confirmed that application of organic fertilizer was effective to increase yield and pharmacological effect through increase of the number of fine-roots with high saponin contents.

Background : The purpose of this study was to evaluate methods to high quality medicinal vegetables, thereby increasing farm income. This study investigated the effects of different organic fertilizer and applicated cultivation on stable foliage production in Peucedanum japonicum Thunberg. Methods and Results : method of application with organic fertilizer were conducted under nine condition compose to control(non application), N-P2O5-K2O (20-12-10)㎏․10 a−1, Hyphal incubation compost(HIC) applied at 851㎏․10 a−1, Hangawi organic compost(HOC) applied at 547㎏․10 a−1, Fermented mixed oil cake compost(FMOC) applied at 380㎏․10 a−1, 60% and 40% of basal fertilizer in March 12, top dressing were four application in April 12, May 12, June 13 and July 12, respectively. Sowing dates were March 23, 2015. Plants were spaced 15 ㎝ apart in rows 15 ㎝ apart with open cultivation. Conclusions : chemical fertilizers < hyphal incubation compost < hangawi organic compost < fermented mixed oil cake compost application, top dressing than 60% in order of 40% additional fertilizer, aerial part as a result were so many such amount of growth as number of leaf per plant, etc., Green leaf yields is untreated control plot (875 kg․10 a−1) compared to 12% from using chemical fertilizers, HIC application 18% higher, HOC application 20% higher, and FMOC application increased to 28% of the most.

Background : Adenophora triphylla var. japonica Hara. is used to cure internal organ, which is called to important one of the five “Sam (ginseng)” oriental medicinal herbs included Panax ginseng, Salvia miltiorrhiza, Sophora flavescens, Scrophularia buergeriana. Korea imported 24 ton 129 thousand dollar for medicinal herbs and 23 ton 29 thousand dollar for food at 2013. This study was carried out to investigate the effect of several organic compost on the growth and root yield of A. triphylla var japonica with organic fertilizer. Methods and Results : Seeds were collected from Adenophora triphylla var. japonica Hara. at last year in nursery of Department of Herbal Crop Research, NIHHS, RDA. Four types organic fertilizer (germiculture (GG), granular mix oil cake (CJ), mixed organic (DJ), plant oil cake (TG)), chemical fertilizer (CF) and non-fertilizer (NF) were treated. It was fertilized on soil before 2 month of planting. Seeds A. triphylla var. japonica were sowed in tray at early March and then were grown during 2 month. It were planted in furrowed nursery at early with 30 × 10 cm planting distance and black vinyl covering condition. Soil chemical property, survival rate, aerial part and root growth characteristics and yield were investigated at harvest time. For main composition analysis, 10 mg of standard (b-sitosterol, lupenone) was taken, 80% MeOH was added to dissolve in 10 ㎖ flask. 0.5, 1, 2, 4 ㎖ was taken from dissolved solution and then was added to 10 ㎖ of 80% MeOH. It was filtered through 0.45 ㎛ filter and then 5 μℓ was taken to make standard solution. Analytical calibration curve was measured to diluted solution within sample concentration. 2 g of ground sample was taken, 40㎖ of 80% MeOH was added to extract by ultrasound sonication during 60 minutes and then it was filtered through 0.2 ㎛ filter to analyze component content. Conclusion : GG and DJ were the most suitable for organic cultivation of denophora triphylla var. japonica. Content of beta-sitosterol showed the highest value in DJ treatment and the lowest value in CF. Content of lupenone showed the highest value in DJ and the lowest value in CJ.

This study was conducted to determine the effects of organic fertilization rates on the nutrient accumulation and recovery in radish (Raphanus sativus L.) as well as growth and yield of radish in Jeju island. An understanding the relationships between organic fertilization rate, crop nutrient recovery and crop yield can assist in making organic fertilizer recommendation which balances crop value and environmental risk in organic cultivation. Nitrogen (T-N), phosphate (P2O5) and potassium (K2O) were applied at 0, 115-35-40, 230-70-80 (standard application rate), 460-140-160, 230-200-100 (recommended application rate) and 158-53-35 kg/ha (customary application rate), respectively as the broadcast application of mixed organic fertilizer (N 4.5% - P2O5 1.5% - K2O 1%) in combination with langbeinite (K2O 22%), 100% at sowing period. The organic fertilizer was made of organic materials like oil cakes. Total yield of radish, as fresh weight of roots, increased with increasing organic fertilizer doses to a maximum at rate of standard or soil-testing application. Nitrogen, phosphate and potassium accumulations of radish increased curvilinearly with increasing organic fertilization rate to a maximum at rate of N 460 - P2O5 140 - K2O 160 kg/ha. However, nitrogen, phosphate and potassium use efficiency of applied organic fertilizer decreased curvilinearly or linearly with increasing organic fertilization rate. Application of organic fertilizer in combination with langbeinite (as a potassium source) had significant effect on the yield of radish. Organic fertilization on a basis of standard or soil-testingapplication rate is recommended for maximun radish yield in organic cultivation.

The present study was conducted to find a way to recycle the coir substrate by investigating changes in its physical and chemical properties based on the number of use year. Specific gravity of unused coir substrate was 0.212 g/cm3 , while it was higher for the substrate used for 2 years. Porosity was different depending on the number of use year. The porosity of unused substrate was 51.9%, but it increased to 68.6% after used for 2 years. In general, physical and chemical properties were better in the coir substrate used for 2 years than in unused one. The number of leaves, leaf area, flesh weight and dry weight of oriental cabbage and lettuce were higher in coir substrate used for 2 years than those in unused one. Whereas, no significant difference was observed between the substrates used for one year and 2 years, indicating that the one time-used wast substrate could be recycled for cultivating vegetables. Growth of the vegetables was improved when organic fertilizer composed of complex organics with different mixing ratios was provided to the coir substrate, compared to untreated plot. The optimum mixing ratio of the wast substrate and complex organics was 2:8(v/v) for fertilization using wast coir substrate. Therefore, coir substrate generally wasted after being used for one time was reuseable by supplying organic fertilizer.

폐자원인 우렁쉥이 껍질을 이용한 환경친화형 유기농 액비의 개발을 위하여 유용미생물(EM)을 이용한 우렁쉥이 껍질의 최적 발효조건과 액비의 특성을 조사한 결과 다음과 같다. EM발효 우렁쉥이 껍질 액비 제조 시 당밀의 투입량이 증가할수록 pH는 낮아지며, EC는 높아지는 것으로 나타났으며, pH는 당밀이 10% 이상 투입된 시험구에서 일정하게 유지되었다. EM발효 우렁쉥이 액비의 총 질소 함량은 발효가 경과할수록 현저히 증가하였으며, 특히 당밀이 15% 이상 투입된 시험구에서 약 220% 증가율을 나타내었다. 인의 함량은 발효 21일차에서 최고치를 나타내었으며, 칼륨의 함량은 발효가 경과할수록 증가하였다. 칼슘, 마그네슘, 나트륨의 함량 역시 발효가 경과할수록 증가하였고 당밀이 15% 이상 투입된 시험구에서 증가율이 우수한 것으로 나타났으며, 칼슘의 함량이 상대적으로 높은 것으로 나타났다. 유해성분(As, Cd, Cu, Cr, Hg, Ni, pb 및 Zn)의 함량은 시험구 모두에서 비료공정규격 기준치에 적합하였다. 우렁쉥이 껍질 액비의 유리아미노산은 총 29종이 검출되었으며 총량은 7,080.94 mg/L를 나타내었고 알라닌이 656.32 mg/L로 가장 높은 함량을 나타내었다. 이상의 결과를 미루어 볼 때 우렁쉥이 껍질을 이용한 액비화에는 당밀이 15% 이상 투입된 조건이 최적인 것으로 판단된다.

The purpose of this study is identify chemical changes in yield components of soil using organic fertilizer for cultivating rice and establish reasonable methods of fertilization. In chemical change, comparing the control plot, the soil using organic fertilizer consistently for three years for cultivating rice showed an increase in pH and available phosphate in the experiment plots provided by the organic fertilizer, increase in spikelet number and 1000 grain weight in terms of yield components, and increase in yield in the three-year experiment plots. As for the quality of brown rice, the rate of head rice showed no difference when compared to that of the control plots. As for protein content, it was difficult to identify a statistical significance between the control plots and the experiment plots in 2009, but since 2010, the protein content of the experiment plots has been lower than that of the control plot, indicating that fertilization of organic fertilizer was effective in terms of rice quality. In conclusion, long-term use of organic fertilizer improved the chemical properties of soil and increased yield when compared to practical fertilization. Reasonable fertilization was identified to fertilize the nitrogen component of organic fertilizer to be a level of 12kg/10a.

연구내용은 마 재배에 적합한 유기물 및 화학비료 시용량을 검토하였으며, 생육기간 동안 마 생장량, 무기성분 및 allantoin 함량의 변화 등을 조사한 결과이다. 마 재배시 유기물시용 효과 단마의 괴경 수량은 화학비료를 줄여 시용할 경우 감소하였는데, 질소시비량이 높은 관행재배(43-28-32 kg/10a, 퇴비 3톤)에서 2,058 kg/10a의 수량을 보인데 비해 화학비료를 시용하지 않고 10톤의 유기물만 시용한 시험구와 유기물 없이 관행시비량의 반량(22-14-16 kg/10a)을 시비한 시험구는 각각 1,322 kg, 1,404 kg/10a로 수량이 감소하였다. 비료를 배량 증시할 경우 시비량 증가에 따른 수량증수 효과가 나타나지 않았다. 유기물 시용과 경운방법에 따른 생육과 수량 비교 유기물만을 시용하여 단마를 재배할 경우 일반적인 쟁기 경운에서는 10톤/10a 이상을 사용하여야 기준시비량 처리구의 86%의 수량을 확보할 수 있었으며, 트렌쳐심경 재배에서는 유기물 10톤/10a 처리에서 기준시비량 처리구의 95%의 수량을 확보하는 효과가 있었다. 질소와 칼리비료 시비량 개선 시험 질소 시비량에 따른 마 지하부 특성 및 수량은 단마와 장마 모두 질소 32kg/10a에서 최고를 보였으며, 마 밭의 적정 질소시비 수준은 최대수량을 보이는 시비수준의 85%인 27kg/10a로 판단되었다. 칼리 비료의 시비량은 마의 수량에 유의적인 영향을 미치지 않았다. 마의 생장변화와 식물체내 무기성분 변화 마의 지하부 생육은 정식 후 3개월 정도 경과 후 괴경 비대가 급속히 이루어졌으며, 생육기간 중 식물체내에 흡수되는 영양분의 요소별 흡수는 괴경 비대가 시작되는 7월 중순경부터 생체 및 건물 중의 증가와 동시에 증가하는 경향이었다. 생육 기간중 allantoin 함량변화 생육 기간중 allantoin 함량은 지상부생육이 활발한 7~8월경으로 주로 지상부에 많이 포함되어 있으며, 지하부 괴경의 allantoin 함량축적에 따른 마 수확적기는 10월 이후가 적당하며 질소시비량에 따른 allantoin 함량은 큰 차이가 없었다.