Galacto-oligosaccharides (GOS) are prebiotics that have a beneficial effect on human health by promoting the growth of probiotic bacteria in the gut, in addition to having various applications in the food industry. GOS are generally produced from lactose in a reaction catalyzed by ß-galactosidase. Synthesis of GOS from whey permeate (WP) (ultrafiltration of whey, concentrated then spray dried) using surface engineered β-galactosidase in Pichia pastoris (P. pastoris) is a novel method to convert waste into a valuable product. Cell-surface display is the expression of peptides and proteins on the surface of living cells by fusing them to functional components of cells. Surface engineered cells have many potential uses. The Flo1p flocculation functional domain, thought to be located near the N terminus, recognizes and adheres non-covalently to cell-wall components such as α-mannan carbohydrates, causing reversible aggregation of cells into flocs.

This study was investigated the quality characteristics and antioxidant of dried Dioscorea bulbifera with various pre-soaking concentrations of oligosaccharide. Dioscorea bulbifera are prepared by additions of 0, 4, 6, 8 and 10% oligosaccharide solution, and dried at 50℃. The effects of pre-soaking percent of Dioscorea bulbifera slices were evaluated by the moisture, soluble solid, pH, titratable acidity, color, browning degree, texture, antioxidant activities and sensory test. According to the percent of pre-soaking oligosaccharide solution was increased, the moisture was increased but soluble solids and titratable acidity were decreased. With respect to the result of colors, Dioscorea bulbifera slices that underwent the 10% pre-soaked process (85.86%) were lighter than control (73.88%). However, the redness and yellowness scores were the lowest than control. The springiness and cohesiveness of texture showed no significant differences among all groups. Gumminess and chewiness of texture results were increased according to per-soaking concentration increase. Also the polyphenol, flavonoid and DPPH (α, α-diphenyl-β-picrylhydrazyl) and ABTS [2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)] radical scavenging activities were significantly increased with increasing immersion concentration. The Dioscorea bulbifera slices supplemented with 6% pre-soaking oligosaccharide solution treatment showed the highest total sensory score. The results of our study indicated that when pre-soaking oligosaccharide solution is used to immerse the Dioscorea bulbifera slice, it has browning inhibition and antioxidant effect.

Glucansucrase is an enzyme classified as a glycoside hydrolase (GH) 70 family, which catalyzes the synthesis of various glucans and glucooligosaccharides with a low molecular weight using sucrose as a donor of D-glucopyranose and maltose as a carbohydrate acceptor. Oligosaccharides are indigestiable carbohydrate with low calories, which have various positive effects on the health of intestinal track. In this study, oligosaccharide produced from Leuconostoc lactis CCK940 was purified and its prebiotics effect was investigated. Leuconostoc lactis CCK940 which isolated from Chinese cabbage kimchi produced oligosaccharide using 5%(w/v) sucrose as a donor and 3%(w/v) maltose as an acceptor. Oligosaccharide produced was purified using Bio-gel P2 column chromatography and DPs of the resulting oligosaccharide were ranged from 3 to 7. When the prebiotics effects of freeze dried oligosaccharide were examined, 1%(w/v) of purified oligosaccharide added to carbohydrate-free MRS showed good prebiotics effects on Lactobacillus plantarum KCCM 12116, L. reuteri KCTC40417, and Bifidobacterium animalis KCCM1209.

Glucansucrase is an enzyme classified as a glycoside hydrolase (GH) 70 family, which catalyzes the synthesis of glucooligosaccharides with a low molecular weight using sucrose as a donor of D-glucopyranose and maltose as a carbohydrate acceptor. In this study, glucansucrase-producing lactic acid bacteria strain was isolated from the fermented foods collected in traditional markets, and the optimum conditions for the oligosaccharide production were investigated. The strain CCK940 isolated from Chinese cabbage kimchi was selected as an oligosaccharide-producing strain due to its high glucansucrase activity, with 918.2 mU/mL, and identified as Leuconostoc lactis. The optimum conditions for the production of oligosaccharides using Leu. lactis CCK940 were to adjust the initial pH to 6.0, add 5% (w/v) sucrose and 10% (w/v) maltose as a donor and acceptor molecules, respectively, and feed 5% (w/v) sucrose at 4 and 8 h of cultivation. When Leu. lactis CCK940 was cultured for 12 h at optimum conditions, at least four oligosaccharides with a polymerization degree of 2-4 were produced.

This study attempted to find an efficient method for the preparation of high-purity galactooligosaccharides (HP-GOS) using β-galactosidase and yeast fermentation. GOS prepared using Lactozym 3000L showed the greatest enhancement in total GOS of the six β-galatosidases tested. GOS alone achieved 51% conversion of initial lactose. GOS production was enhanced by fermentation with commercial yeast (Saccharomyces cerevisiae); its concentration reached 71% after 36h fermentation with 8% yeast. Component sugar analysis with HPLC indicated that HP-GOS fermented with S. cerevisiae showed significantly increased levels of 4’/6’-galactosyllactose and total GOS as well as a significantly decreased glucose level. HP-GOS facilitated the growth of Lactobacillus sp. (L. acidophilus and L. casei) and Bifidobacterium sp. (B. longum and B. bifidum). In sum, high-purity GOS has been successfully produced through both an enzymatic process and yeast fermentation. GOS encourages the growth of bacteria such as Lactobacillus and Bifidobacterium that may be beneficial to human gastrointestinal health.

Burkholderia는 Riptortus pedestris의 특이적 중장기관인 M4의 crypt에서 공생 하고 있으며 숙주의 성장과 건강에 긴밀하게 영향을 미친다. 그람음성균인 Burkholderia는 가장 바깥에 lipid A-core oligosaccharide-o-antigen로 이루어진 lipopolysaccharide를 가지고 있으며, 본연구진의 선행연구에서 공생 Burkholderia 가 숙주와 공생관계를 형성 할 때 o-antigen이 소실되는 것을 알아내었다. o-antigen 이 소실된 공생균은 core oligosaccharide (core) 부분이 가장 밖에 노출됨으로, core 는 숙주와의 공생에 중요한 영향을 끼칠 것이라 가정했다. 그래서 우리는 core의 공 생에서의 역할이 무엇인지 규명하기 위해 Burkholderia의 core의 생합성 하는 유전 자가 결손된 △glycosyltransferase 1 (GT1), △glycosyltransferase 2 (GT2), △ waaF, △waaC 변이 균주를 구축하였다. 전기영동 분석에서 구축한 변이 균주의 core는 야생형 Burkholderia와 다르다는 사실을 확인하였고, 야생형 및 변이균주 를 R. pedestris에 감염시킨 후 공생균과 R. pedestris에 미치는 영향을 보았을 때 5 령 약충에서 core가 많이 손실된 △waaF, △waaC 변이 균주의 감염 밀도는 야행성 균주보다 더 낮게 나타났으며. 이 변이 균주를 공생균으로 가진 곤충은 발달이 느리 고 체중이 감소하였다. 특히 core가 가장 많이 손실된 △waaC를 공생균으로 가진 곤 충은 가장 짧은 신장과 가장 낮은 생존율을 보였다. 이 사실로 core는 Burkholderia 와 R. pedestris가 서로에게 미치는 해로운 영향을 보호함으로써 Burkholderia와 R. pedestris의 공생관계를 원활하게 한다는 새로운 사실을 발견하였다. P071

This study was performed to determine the optimal composition of a muffin added with jerusalem artichoke powder and oligosaccharide. The experiment was designed based on CCD (central composite design), and evaluation was carried out by means of RSM (response surface methodology), which included 10 experimental points with three replicates each for the two independent variables jerusalem artichoke powder and oligosaccharide. The experimental muffin was prepared according to a traditional recipe, except that the flour was partially replaced by jerusalem artichoke powder (5, 15, or 25%) and the sugar was partially replaced by oligosaccharide (25, 50, or 75%). Using F-test, height, moisture, a-value, b-value, springiness, cohesiveness, texture, and overall acceptability were expressed as a linear model, whereas volume, pH, L-value, appearance, flavor and taste were expressed as a quadratic model. Increased amounts of jerusalem artichoke powder led to reduction of sensory scores for appearance, flavor, taste, texture, and overall quality. The optimum formulation determined by the numerical and graphical methods were similar: jerusalem artichoke powder 10.99%, oligosaccharide 71.40%.

Physicochemical and sensory characteristics of the green tea dasik varying the added value of oligosaccharide were investigated and the products were surveyed by elementary school students regarding the acceptability. Protein and ash contents were significantly higher and fat contents were significanlty lower in the samples with oligosaccharides compared to those in commercial ones(p<0.05). Commercial samples had significantly higher values in all of the textural characteristics using textural analyzer compared to those of the developed dasik sample groups. In analytical sensory evaluation, varying the amount of oligosaccharide, commercial samples had significantly higher values of sweetness, hardness, and cohesiveness and lower values of savory aroma and falvor compared to those of developed dasik(p<0.05). Savory aroma and flavor were highly negatively correlated with textural hardness, springiness, chewiness, gumminess, and cohesiveness(p<0.01), while sensory sweetness and hardness were significantly positively correlated with textural springiness(p<0.05), chewiness, gumminess, and cohesiveness(p<0.01). When surveying elementary school students regarding the acceptability of green tea dasik and commercial samples, green tea dasik 2 was the most preferred with significance in color. Regarding aroma, green tea dasik 1 received the highest acceptability (p<0.05). In the flavor, both dasik 1 and 2 received higher score than those of the commercial dasik. In overall acceptability, both green tea dasik 1 and 2 were preferred to the commercially sold ones with significance(p<0.05).

This study is based on the utilization of pine mushroom for processing products by development of pine mushroom Jung-Gwa. The results were summarized as follows: Pine mushroom Jung-Gwa were prepared with four different amounts (20, 30, 40, 50%) of honey. Pine mushroom Jung-Gwa color value was the highest by 20% soaking honey-water product and 40% soaking honey-water product was the lowest. Strength and hardness of 20% soaking honey-water product was the highest. Appearance to product of 50% soaking honey-water was the highest preference by all age of except 20's. The honey content of more and more external appearance preference was became higher. Product of 40% soaking honey-water was best by flavor of pine mushroom Jung-Gwa. A sugary tastes comparison 50% soaking honey water to 40% soaking honey water was not distinction. The texture was appeared a statistically significant difference by products of 20%, 30% soaking honey-water. Product of 20% soaking honey-water was favorite in 20's and product of 30% soaking honey-water was favorite in 30's. In overall preference test for pine mushroom Jung-Gwa of 40% soaking honey water was preferred by all age. Oligosaccharide helped geriatric diseases and cheaper. Thus this study used oligosaccharide but the result was not a statistically significant difference of pine mushroom Jung-Gwa products quality by comparison honey-water to oligosaccharide. Therefore oligosaccharide utilization products was thought worth a lot more by processing price and functional.

고온기 분식 칼랑코에 재배에 있어 문제는 고온 스트레서에 의한 생장의 억제로, 본 연구는 지하부 및 지상부의 생장을 촉진을 시킬 수 있다고 알려진 근권 생장촉진미생물(Pseudomonas sp. B와 Pseudomonas sp. D2)을 선발하여 사용하였으며 천연물로서는 alginoligosaccharide와 glucosamine oligosaccharide를 사용하였다. 또한 이들의 AG-용액과 단일 및 복합 처리하여 재배 온도차이를 부여한 경우 칼랑코에의 생장에 미치는 영향을 조사하였다. 상이한 지하부 온도조건은 25℃, 30℃ 및 35℃로 처리하여, 2주후 초장, 엽장,엽폭, 엽면적, 엽중, 지상부 생체중, 근장, 근중을 조사하였다. 25℃처리구에서는 초장, 엽면적, 엽중, 근중 모두 Pseudomonas sp. B와 glucosamine oligosaccharide를 혼합한 처리구가 가장 높았고, 30℃의 경우 초장과 엽중은 glusosamine oligosaccharide처리에서, 엽면적과 근중은 Pseudomonas sp. D2와 algin-oligosaccharide를 처리구에서 가장 좋은 생장 효과를 나타내었다. 35℃의 경우는 초장, 엽면적, 엽중, 근중 모두 Pseudomonas sp. B와 glucosamine oligosaccharide를 혼합한 처리구에서 효과가 컸다. 이상의 결과로 보아 고온기 칼랑코에의 분화 재배시 문제가 되는 생장억제현상은 천연제재와 미생물 제재를 혼합처리 함으로써 크게 개선시킬 수 있었다.

본 연구에서는 참다래 식초 개발을 위하여 초산발효 조건 및 올리고당 첨가에 따른 품질 특성을 조사하였다. 그 결과 초기알코올 농도 6%, 초기산도 1%에서 7일 동안 발효시켰을 때 총산이 가장 높게 나타났다. 올리고당 첨가에 따른 영향에서 총산은 올리고당 15% 첨가구에서 5.03%로 가장 높게 나타내었다. 참다래 식초의 유리당은 fructose, glucose 및 maltose가 확인되었으며 총 유리당은 올리고당 첨가량이 많을수록 높게 나타났다

This study was conducted to identify the soluble carbohydrates in soybean seeds using on-line HPLC-RID-ES/MS and HPLC behavior, and to deter­mine their contents for high quality soybean breeding. The monosaccharide (glucose) and three oligosaccharides (sucrose, raffinose, and stachyose) were identified in Korean soybeans by their chromatographic behavior and results of on-line HPLC-RID-MS with Electro­spray Ionization mode. On the basis of HPLC with a RID detector, the 32 Korean major soybeans contain 0.37~pm0.26~% glucose, 4.55~pm0.91~% sucrose, 1.19~pm0.19~% raffinose, and 2.72~pm0.37~% stachyose on a dry basis. In 468 soybean germplasms, the ranges of glucose, sucrose, raffinose, and stachyose were 0.03 - 0.98~% , 2.33 - 6.96~% , 0.08 -1.87~% and 0.75 - 3.18~% , respectively. Among 500 soybean samples, oligosaccharide contents of 32 Korean major cultivated soybeans and 468 soybean germplasms were varied 5.83 - 10.06~% and 3.66 - 10.32~% , respectively. The composition of glucose, sucrose, raffinose, and stachyose in soluble carbo­hydrates of 500 soybean samples were 2.07 ~pm 1.75~% , 58.01~pm5.82~% , 10.13~pm2.28~% and 29.80~pm4.54~% , respectively. Sucrose appeared to be most prevalent in soy­bean soluble carbohydrates.

To enjoy a healthy life, it is important to have a well-balanced diet. However, in today's society, there is an increase in the consumption of preprocessed foods and frequency of eating out. Also the western diet, which is becoming move popular worldwide, contains relatively high levels of protein and fat, and a low amount of fiber, Furthermore, the increased availability of favorite foods has created a condition were the individual diet is less variable. With these conditions, it is difficult to maintain a diet that is nutritionally balanced. With these unbalanced diets, which are difficult to change, there has been an increase in adult disease and health problems, such as colon and breast cancer, It is speculated that metabolites for carcinogens are produced from diet components and that intestinal bacteria contribute to the production of these metabolites. Therefore, it is necessary to evaluate the relationships between health, diet, and intestinal microflora. Soybean oligosaccharide is composed of water-soluble saccharides that have been extracted from soybean whey, a by-product from the production of soy protein. This is mainly a mixture of mono-, di-, tri-, and tetrasac-charides, with the principle components being the oligosaccharide raffinose and stachyose. When consumed by humans, the oligosaccharides cannot be digested in the human duodenal and small intestinal mucosa, and these are selectively utilized by beneficial bifidobacteria in intestines. The results of acute and subacute toxicity tests, soy-bean oligosaccharides were nonpoisonous. Soybean oligosaccharides promote the growth of indigenous bifido-bacteria in the colon which by their antagonistic effects, suppress the activity of putrefactive bacteria. Also, they reduce toxic metabolites, detrimental enzymes and plasma lipid, and increase in the frequency of bowel evacuation and fecal quantities. Consequently, soybean oligosaccharides as functional foods components have potential roles in the prevention and medical treatment of chronic adult diseases. The study of processing property and physiological function of soybean oligosacchavides and development of high oligosaccharide variety allow the creation of new and exciting foodstuffs that aye functional healthy.

This study was carried out to establish the analysis condition for oligosaccharides of soybeans (Glycine max L.) and to assay quantitative evaluation of oligosaccharide contents in soybean varieties and soybean sprouts. 55% acetonitrile solution used as a