Spinach (Spinacia oleracea L.), a green leafy vegetable, is well known as a functional food due to its biological activities. Vascular calcification is associated with several disease conditions including atherosclerosis, diabetes, and chronic kidney disease (CKD), and is known to raise the risk of cardiovascular diseases related morbidity and mortality. However, there are no previous studies that have investigated the effects of fermented spinach exract (FSE) against aortic and its underlying mechanisms. Therefore, this study investigated the effects and action of possible mechanisms of FSE on inorganic phosphate (PI)-induced vascular calcification in ex vivo mouse aortic rings. PI increased vascular calcification through calcium deposition in ex vivo aortic rings. FSE inhibited calcium accumulation and osteogenic key marker, runt-related transcription factor 2 (Runx2), and bone Morphogenetic Protein 2 (BMP-2) protein expression in ex vivo aortic rings. And, FSE inhibited PI-induced extracellular signal-regulated kinase (ERK) and p38 phosphorylation in ex vivo aortic rings. These results show that FSE can prevent vascular calcification which may be a crucial way for the prevention and treatment of vascular disease association with vascular calcification.

고품질의 작물을 재배하기 위해 광은 필수적인 환경조건이 다. 겨울철에는 다른 계절에 비해 일사량이 저조하므로 보광 처리를 이용해 작물의 생육과 수확량을 증대시킬 수 있다. 본 연구는 약광기 동안 고추 온실재배를 위한 경제적인 보광 광 원을 선발하기 위해 수행되었다. 풋고추(Capsicum annuum ‘Super Cheongyang’)는 2019년 9월 5일에 정식하였다. 보광 처리는 2020년 1월 1일부터 2020년 3월 31일까지 수행되었 다. White LED(R:G:B = 5:3:2, W LED), RB LED(red:blue = 7:3, RB LED), 고압나트륨등(high pressure sodium lamp, HPS)을 광원으로 사용하였다. 무처리를 대조구로 사용하였 다. 고추의 초장, SPAD, 마디 수는 보광 광원에 따른 유의적인 차이가 없었다. 그러나 분지 수는 RB LED 광원에서 가장 많 았다. 또한 보광은 고추의 광합성을 증가시켰으며, 특히 RB LED에서 보광기간 동안 가장 높은 광합성률을 보였다. 또한 고추의 수확량은 보광처리에서 증가하였고, RB LED는 다른 광원에 비해 가장 높은 수확량을 보였다. 소비전력은 W LED 가 가장 높았고 HPS 조명이 가장 낮았다. 경제적인 측면에서 RB LED를 이용한 보광처리는 다른 광원에 비해 높은 경제성 을 가졌다. 결론적으로 이러한 결과는 고추 온실에서 약광기 동안 보광 광원으로 RB LED를 사용하는 것이 수확량과 경제 성을 향상시킬 수 있을 것으로 판단된다.

글루탐산은 식물의 생장과 발달에 중요한 역할을 하는 필수 아미노산의 전구체이며, 저온 보호 물질로 이어지는 생합성 경로를 자극하여 저온 피해를 줄이는 생물자극제 중 하나이 다. 본 연구에서는 저온 스트레스 조건에서 글루탐산 엽면 처 리가 배추에 미치는 영향을 평가하였다. 글루탐산 2가지 엽면 시비 농도(0 및 10mg·L-1)와 3가지 주/야간 온도 수준(11/－1 °C extremely low, E; 16/4°C moderately low, M; 21/9°C optimal, O)을 결합하여 6개의 처리가 수행되었다. 글루탐산 의 엽면 처리는 정식 후 10일에 1회 살포하고, 글루탐산 처리 직후 온도 처리는 최대 4일 동안 실시하였다. 처리 4일 후, ABA, PA, DPA 및 ABA-GE 함량은 M 처리에서 Glu 0 처리 보다 Glu 10 처리에서 함량이 더 높았다. Glucose 함량은 E 및 Glu 10 처리에서 가장 높은 반면(52.1mg·100g-1 dry weight), fructose 함량은 O 및 Glu 0 처리에서 함량이 가장 높았다 (134.6mg·100g-1 dry weight). GLP, GBS, 4MGBS, GNBS 및 GNS 함량은 E 및 Glu 10 처리에서 모든 처리 중 가장 높았 다(0.72, 2.05, 1.67, 9.40 및 0.85μmol·g-1 dry weight). 처리 2일 후 E 및 Glu 10 처리의 PA와 DPA함량에서 급격한 변화 를 확인하였고, 몇몇 개별 glucosinolate 함량(GLP, GBS, 4MGBS, GNBS 및 GNS)은 저온과 글루탐산 처리에 따라 유 의적 차이를 확인할 수 있었다. 또한, fructose는 glucose 대신 fructan을 에너지원으로 사용하였기 때문에 처리 4일후 E와 M처리에서 O 처리에 비하여 유의적으로 낮은 함량을 보였다. 따라서, 저온과 글루탐산 엽면 처리에 따른 PA, DPA, glucose, fructose 및 개별 glucosinolate 물질들의 변이를 확 인하였지만, 저온과 글루탐산의 효과에 관한 명확한 상관관계를 평가할 수는 없었다. 배추과 작물은 호냉성 채소로서 저 온에 민감하게 반응하지 않고, 대부분 내한성을 가지고 있기 때문으로 판단된다.

This study evaluated the migrant and residue tests of lead (Pb), cadmium (Cd), nickel (Ni), arsenic (As), and antimony (Sb) in 70 tumbler samples. The migration levels of hazardous metals in all the samples were within the migration limits outlined in the Korean standards and specifications for utensils, containers, and packages. Moreover, in all the tumbler samples, only Ni was detected in 0.5% citric acid solution of a food stimulant. The maximum level of Ni 0.0144 mg/L was 14.4% of the migrant specification (not more than 0.1 mg/L), which was relatively safe. The 0.5% citric acid solution was eluted at 4oC, 70oC, and 100oC for 30 min, and only Ni was detected while testing for migration levels according to the temperature variations; all temperature conditions conformed to the standards. The level of Ni migration increased significantly with increasing migration temperature. Regarding the residue level outside the paint-coated tumbler samples, the Pb level was found to range from N.D. to 20638.1323 mg/kg. The risk of Ni was further estimated to be at a safe level of 0.00 to 0.01% compared to the %TDI as a result.

본 연구는 딸기재배시 전용 고설베드를 사용하지 않고, 일 반 수경재배 시설을 이용하여 코이어 배지를 베드에 올려 재 배하는 방법을 구명하기 위해서 실시하였다. 토마토나 파프 리카를 재배하는 시설재배 베드에 코이어의 칩과 더스트 비율 이 5:5인 코이어 배지 1겹 처리(높이 10cm; A), 2겹으로 쌓은 처리(높이 20cm; B), 코이어의 칩과 더스트 비율이 7:3인 코 이어 배지 1겹 처리(높이 15cm; C)와 대조구로는 딸기 전용 플라스틱 화분(Control) 처리구로 하였다. 생육특성은 코이 어 배지 높이별로는 유의성이 없었고, 플라스틱 화분에서 재 배한 것이 작은 경향을 보였다. 딸기 잎의 광합성율은 처리별 로 14.68－15.76μmol CO2·m-2·s-1로 통계적인 유의성은 없 었고, 뿌리의 근활력은 배지 용량이 컸던 C와 B 처리구가 A 처리구와 대조구보다는 높은 것으로 나타났다. 과장과 과폭 은 각각 4.04－4.13cm와 3.26－3.34cm로 통계적인 유의성 이 없었고, 과장과 과폭 비율은 대조구가 1.27로 A－C 처리 구의 1.23－1.24보다 뾰쪽한 형태이었다. 딸기 1주당 수확과 수는 C 처리구가 4.4개로 가장 적었고, 대조구, A, B 처리구의 6.2－6.5개로 처리간 유의성은 없었다. 상품수량 과수는 A 처 리구가 74개로 가장 많았고, C 처리구가 53개로 가장 적었으 며, 1주당 수량은 A 처리구가 72.38g으로 가장 컸고, C 처리 구가 48.69g으로 가장 작았다. 이와 같은 결과는 딸기재배 시 전용재배 시설을 설치하지 않고, 기존의 토마토나 파프리카 수경재배 시설에서 코이어 배지를 활용하여 딸기재배를 할 수 있다는 것을 나타낸다. 다만 C 처리구에서 수량이 감소한 것 은 칩과 더스트 비율이 7:3으로, A와 B 처리구의 칩과 더스트 비율이 5:5와 다른 것이 원인으로 추정되며 칩과 더스트 비율 에 따른 추가 연구가 필요하다.

Recently, the number of cases of fire spreading due to exterior materials of buildings is increasing. Due to the nature of modern architecture, which emphasizes the aesthetics of buildings, because buildings pursue a splendid appearance, they are inexpensive and have relatively good insulation performance, but an increasing number of buildings are adopting insulation materials that have poor fire safety performance. The risk of spread is also greatly increased. Since the exterior wall of a building is made of a variety of materials and structures, it is composed of a combination of several elements, including materials such as insulation and finishing materials. Therefore, it was determined that it was necessary to introduce a more systematic evaluation method for building exterior materials, and to improve the system reflecting this, away from the existing evaluation method that only checked the fire safety performance of finishing materials.

This study was to investigate an analytical method for determining dieckol content in Ecklonia stolonifera extract. According to the guidelines of International Conference on Harmonization. Method validation was performed by measuring the specificity, linearity, precision, accuracy, limit of detection (LOD), and limit of quantification (LOQ) of dieckol using high-performance liquid chromatography–photodiode array. The results showed that the correlation coefficient of calibration curve (R2) for dieckol was 0.9997. The LOD and LOQ for dieckol were 0.18 and 0.56 μg/mL, respectively. The intra- and inter-day precision values of dieckol were approximately 1.58-4.39% and 1.37-4.64%, respectively. Moreover, intra- and inter-day accuracies of dieckol were approximately 96.91- 102.33% and 98.41-105.71%, respectively. Thus, we successfully validated the analytical method for estimating dieckol content in E. stolonifera extract.

In this study, a two-stage electrostatic precipitator (ESP) was developed using a novel automatic dry cleaning device to reduce the ultrafine particles in subway stations. Collection efficiency was evaluated with a pilot scale ESP (1.2m× 1.2m) and the scale of the test duct was half of the subway air handling unit. The maximum collection efficiency for 0.3 μm particles was 96.9%. In addition, we studied a method of automatic dry cleaning for maintenance of the ESP. The cleaning efficiency was analyzed according to the cleaning flow rate for each particle loading amount to achieve a recovery rate over 90%. In addition, we derived the equation to estimate the reduction in collection efficiency according to the particle loading amount. It was confirmed that the performance of the contaminated ESP was restored to the initial state by the automatic dry cleaning in this study and that the electrical energy consumption was 5 times lower compared to utilizing conventional water cleaning.

Background/Aim: In gallbladder cancer (GBC), gender differences in incidence and mortality rates have been reported with geographic variation. However, there is little known about sex-related difference in GBC prognosis. This study compares prognostic factors according to gender for GBC. Methods: We searched clinicopathological factors in all stages of 952 GBC patients from seven medical centers in Korea. A total of 927 patients were enrolled and surgery with curative resection was performed in 499 patients. Results: Carbohydrate antigen (≥37 U/mL) was a significant prognostic factor in both females and males (odd ratio [OR], 4.30; 95% confidence interval [CI], 3.13-5.89; p<0.001). Age was a significant factor only in female patients, elderly patients were associated with low resectability and the likelihood of T-stage >2; an independent predictor of poor prognosis via multivariate analysis (OR, 1.03; 95% CI, 1.01-1.05; p=0.005, OR, 1.05; 95% CI, 1.02-1.08; p=0.002). Body mass index (BMI) also showed gender difference, and lower BMI (≤25 kg/m2) was the significant good indicator of multivariate analysis for lymph node metastasis in female patients (OR, 0.42; 95% CI, 0.23-0.77; p=0.005) but, the significant poor indicator of univariate analysis for advanced T-stage in male (OR, 2.79; 95% CI, 1.40-5.54; p=0.003). Conclusions: These results suggest that there is a possibility of gender difference in GBC prognosis. Age and high BMI were poor prognostic factors for curative resection for female GBC patients.

In this study, the adsorption/desorption performance of toluene was evaluated using zeolite adsorbent to replace activated carbon with one-off and ignition characteristics. For the proper operation of the VOCs adsorption/desorption and condensate recovery steps, the operating range by various adsorption/desorption temperatures was selected. The adsorbent is a bead-type zeolite, which was put into an adsorption tower of 10 LPM scale. As a result, it was demonstrated that 0.079 mg/g was adsorbed at a low temperature (20°C) during adsorption. In the case of desorption, it was found that VOCs adsorbed on the adsorbent were completely recovered after the desorption operation at 220°C for about 160 minutes. However, in the heating rate step for desorption, it was not possible to maintain an appropriate heating rate by filling the tower with zeolite. This was complemented by applying a copper plate with high thermal conductivity, and it was shown that the time was shortened by about 10 minutes or more. When VOCs are emitted at high concentrations during the desorption process, they can be reused as energy resources through low-temperature maintenance, and a condensation method was attempted. The efficiency of condensing chiller (cooler) with temperature control and liquid nitrogen condensing was compared. It was found that the chiller condensing efficiency increased as the temperature decreased. In the case of liquid nitrogen condensation, the liquid nitrogen temperature was maintained at -196°C, showing a stable efficiency of 90%.

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Newborn piglets are routinely subjected to several treatments shortly after birth, one of which is tail-docking. Tail-docking, which is carried out without an anesthetic when the piglet is 3 to 4 days old, is intended to prevent the severe injuries that can occur when pigs bite each other’s tails. The study was performed in slaughterhouses to determine how much tail-docking prevented tail-biting and how much it caused clinical problems such as amputation neuroma. One thousand pigtails were collected from 3 slaughterhouses in different provinces and tail length and tail-biting injuries were examined. Among them, 659 tail tissues were examined for clinical problems like amputation neuroma. The collected tails were divided into 3 groups according to the length of the tail, which was defined as long (n=136, 75% of the tail remained compared to the referred intact tail length of 30.6±0.6 cm of crossbred Landrace×Yorkshire dam×Duroc sire; LYD), medium (n=694, 50% of the tail remained), and short (n=170, 25% of the tail remained). The results showed that 4.3% of 1000 tails had biting injuries and 58.7% of 659 tails had amputation neuromas. There was no significant association between the tail-biting injury and tail lengths (p=0.953). However, the tail-biting injuries differed significantly by the province (p<0.001), and the frequency of amputation neuromas also appeared more frequently in longer tail lengths (p<0.001). The results indicated that tail-biting behavior was not influenced by tail-docking but might be influenced by the housing system and/or management practices.

최근 도시 과밀화와 건축물 고층화로 인해 빌딩풍에 의한 시설 피해는 물론 보행자의 안전을 위협하는 풍환경에 대한 우려 가 커지고 있다. 빌딩풍 피해 저감을 위한 방안으로 건축물 주변 풍환경 평가를 통해 도시의 풍환경을 개선하는 것이 중요하다. 이를 위해 풍동실험을 대체하거나 보완하는 수단으로 최근 전산 유체역학 기법 (CFD)의 적용이 받아들여지고 있으며, 국토교통부가 보행 자 풍환경 평가를 위한 CFD 활용 가이드라인을 제시한 바 있다. 본 논문에서는 이 가이드라인의 적정성을 평가하기 위한 전산해석을 수행하였다. CFD 결과의 검증을 위해 일본 건축학회가 제시한 모형과 풍동실험 결과를 사용하였다. 평가결과 일부 위치에서 풍동 실 험값과 CFD 결과의 풍속 차이는 있으나 격자의 상세도가 정확도에 미치는 영향과 CFD를 이용한 보행자 풍환경 평가 가능성을 확인 하였다. 또한, 건물 주위의 상승풍, 하강풍 및 와류 등으로 인한 돌풍이 잘 모사되고 있음을 확인하였다.

This study evaluated whether fevernill, which is primarily composed of acetaminophen, vitamin C, and anhydrous citric acid, is effective in relieving stress caused by foot-and-mouth disease virus (FMDV) vaccination in calves. Three-month-old calves from a farm in Korea were randomly assigned to one of three groups (n = 10 per group: control [untreated], group A [FMDV vaccination], and group B [FMDV vaccination + addition of fevernill 0.2% to feed for five days after vaccination]). Body weights, antibody formation, clinical symptoms, serum parameters were measured in the 14 days following vaccination. Total weight gain and average daily gain during the experiment period were group A (12.00±1.15 kg, 0.86±0.08 kg/day) compared to group B (13.57±0.98 kg, 0.97±0.07 kg/day) was significantly improved (p<0.05). There was no significant difference in the formation of antibodies against the FMDV vaccine between group A and group B, though the antibody value of group B tended to be higher than that of group A. The pre-inflammatory cytokine TNF-α value was 74.47±19.26 pg/ml in group A and 59.05±11.88 pg/ml in group B on the 5th day of the experiment, which was significantly lower in group B than in group A (p<0.05). Also, cortisol concentrations were significantly lower in group B than in group A (p<0.05). In conclusion, the feeding of fevernil is judged to be helpful in mitigating the side effects caused by FMD vaccination, and thus it is thought to be able to prevent the decrease in productivity caused by vaccination.

인삼은 다양한 국가에서 과거로부터 민간의약적으로 사용해 온 유용한 한약자원으로, 동북아시아와 북아메리카에서 서식하고 있다. 인삼의 학명은 Panax ginseng C. A. Meyer로 Araliaceae에 속하고 약용 부위는 곁뿌리를 제거한 뿌리 부분이다. 본 연구에서는 전세계에 분포하는 인삼의 종류와 명칭을 정리하고, 동북아시아 공정서 내 인삼의 기원들을 알아보았다. 다양한 인삼종의 전초의 형태를 비교하였고, 여러 문헌 정보를 바탕으로 각국 인삼종의 약리학적 효능을 정리하였다. 현재 한국에서 재배되고 있는 고려인삼의 품종은 약 15종이며, 이들 중 재래종(자경종), 천풍, 연풍, 금풍, 산양삼의 형태학적 특징을 비교하였다. 한약재 활용 가능한 인삼을 수확하기 위해서는 오랜 기간 재배해야 하며, 그 조건도 까다롭다. 약리학적으 로 가치가 있는 인삼종의 시장 수요를 충족하기 위한 대량증식 방법으로 기내조직배양이 활용될 수 있다. 따라서, 각 인삼종의 종자를 수집하여 크기를 측정하고 형태를 비교하였고, 종자를 횡단면으로 절단해 배의 형태를 관찰했다. 각 인삼종의 종자를 활용하여 기내배양을 진행하였고, MS+GA 1.0mg/L 조건에서 발아율은 미국삼이, 생존율은 금풍이 가장 우수한 것으로 확인 되었다. 인삼의 한 종류인 산양삼은 자연에서 성장 속도가 느리고, 재배조건이 까다롭다는 특징을 가지고 있다. 따라서, 산양삼을 수경 재배해, 해당 인삼종에서 발견되는 다양한 미생물종을 동정하였다. 기내배양을 통한 4종의 인삼종 종자 발아 실험에서도 다양한 미생물종이 확인되었고, rDNA 염기서열 분석을 통해 동정하였다. 본 연구는 한국한의학 연구원이 제공하는 전통의학정보포탈(KIOM Oasis Portal)과 농업진흥청 원예특작과학원의 인삼특작부에서 각 인삼종의 종자를 분양받아 진행되었 다. 해당 논문은 기내배양을 통한 인삼의 대량생산 체계 구축 연구의 초석이 될 것으로 사료된다.

PURPOSES : This study evaluates the long-term performance of the asphalt overlay designed by the Seoul pavement design method which determines overlay thickness by considering existing pavement conditions, traffic volume, and bearing capacity of the pavement. METHODS : A total of 76 sections including 17 control sections and 59 design sections were constructed under various traffic conditions, overlay thicknesses and asphalt mixtures. The performance of the pavements has been monitored up to 60 months in terms of surface distresses, rutting, and longitudinal roughness. The service life of the pavements was estimated to be the period when the Seoul pavement condition index (SPI) becomes 6.0, i.e., a rehabilitation level. RESULTS : Overall, the service life of the pavements was 72 months in the control and 120 months for the design sections. For relatively thinner overlay sections than designed, the service life reduced significantly; 36 months for 15cm thick overlay and 120 months for 25cm thick overlay. The service life of the pavement in the bus-only lane was 78 months, which is 30 months shorter than that in mixed-traffic lanes. Out of the bus-only lanes, 56% of the pavement along bus stop was deteriorated early to be a poor condition while only 2% of the pavement in a driving lane was degraded to be poor. The overlay with Stone Mastic Asphalt (SMA) in the wearing surface had 38% longer life than that with conventional dense graded mixtures. CONCLUSIONS : Most of the overlays sections designed by the Seoul pavement design method were expected to survive 10 years, except for bus-only lanes. The control sections having 5 to 10 cm thick overlays showed significant lower performance than the design sections. Thus proper thickness and materials considering the characteristics of existing pavement and traffic volumes should be applied to secure the service life of overlays.

Purpose: This study aimed to understand the COVID-19 vaccination experience of nursing students and explore its importance. Methods: A descriptive qualitative study and content analysis was conducted. The participants were 14 nursing students who expressed their rejection of COVID-19 vaccination. Data were collected through in-depth interviews from November 24, 2021, to January 30, 2022. Results: The COVID-19 vaccination experience of nursing students included four themes: “nostalgia for everyday life before COVID-19”, “hesitancy to get vaccinated against COVID-19”, “different thoughts about COVID-19 vaccination”, and “future direction of COVID-19 vaccination”. Conclusion: The COVID-19 vaccination experience of nursing students may be due to a lack of trust in COVID-19 vaccination. Therefore, accurate information about COVID-19 vaccination should be provided to lower the vaccination hesitancy of nursing students.