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        검색결과 333

        181.
        2009.04 KCI 등재 구독 인증기관 무료, 개인회원 유료
        This study was carried out to determine the quality characteristics of Jeju mandarin orange jellies containing sugar (control) and sugar derivative sweeteners (erythritol, isomaltooligosaccharide, sorbitol, and xylitol) to be consumed by the elderly. The gelling agents were carrageenan and gellan gum at the concentration of 0.27%, respectively. The average age of the subjects for the acceptance test was 78. The lightness (L), redness (a), and yellowness (b) values of the carrageenan gel were higher than those of the gellan gum gel using the same sugars. The L, a, and b values of the carrageenan gel made with sugar derivative sweeteners were higher than those of the respective gel made with sugar. Whereas there were almost no differences between the color values of the gellan gum gels made with sugar and sugar derivative sweeteners, respectively. The gelling and melting temperatures of the carrageenan gel with erythritol were highest and those of the respective gel with xylitol were lowest among the sugar derivative sweeteners. The gelling and melting temperatures of the gellan gum gels with erythritol and isomaltooligosaccharide, respectively, were lower, and those of the respective gel made with xylitol were higher compared to that with sugar. There was no break down in the gellan gum gel. The break down rate of the carrageenan gel made with isomaltooligosaccharide was highest and that of the respective gel with xylitol was lowest among the sugar derivative sweeteners. The hardness, adhesiveness, springiness, and chewiness of the gellan gum gel were greater compared to the carrageenan gel. The adhesiveness levels of the carrageenan gels made with sugar derivative sweeteners were lower, and their springiness was higher than the respective gel with sugar. All the TPA characteristics of the gellan gum gels made with erythritol and xylitol, respectively, were higher than the respective gel with sugar. The rupture properties of the gels were the same as their TPA characteristics. The sensory acceptance of the carrageenan and gellan gum gels made with sugar were highest among all the sweeteners. There were no significant differences in the sensory acceptance scores of the carrageenan gels made with xylitol, erythritol, and sugar, respectively, and there were also no significant differences in the sensory acceptance scores of the gellan gum gels made with xylitol, sorbitol, and sugar, respectively. Xylitol was preferred in both the carrageenan and gellan gum gels. Thus, xylitol appears to be an appropriate alternative sweetener to sugar in Jeju mandarin orange jelly for consumption by the elderly, with regard to the acceptability and stability of the gel.
        4,000원
        182.
        2009.02 KCI 등재 구독 인증기관 무료, 개인회원 유료
        Intracellular reactive oxygen species(ROS) produced in a various pathologic state was known to intermediate many cellular response such as inflammation. Recently, low level light irradiation by HeNe laser used in many clinical field could improve inflammatory state by scavenging intracellular ROS through photo-detachment/dissociation process. The purpose of this study is to investigate the differential effects of blue and red light irradiation on ROS scavenging effects. Immortalized human oral keratinocyte HaCat cells were used. Phorbol 12-myristate 13-acetate(PMA) was treated for inflammation. Red(635nm) and blue(470nm) light irradiation was carried out. To asses the intracellular ROS by light irradiation, confocal microscopic and flow cytometric assay with DCF fluorescence for total ROS and ESR spectrometry of DMPO-O2 - for superoxide anion were caried out. And microarray was performed for mRNA expression level. Released intracellular total ROS in PMA treated HaCat cell lines was dissociated efficiently by red light irradiation, while blue light irradiation did not. Rather, blue light irradiation increased ROS formation. For superoxide anion generated the first synthetic form of ROS, red light irradiation reduced its amount but blue light irradiation did not. In the mRNA expression in line with cyclooxygenase(COX) pathway, prostagrandin endoperoxide synthase 1(PTGS 1), prostagrandin endoperoxide synthase 2(PTGS 2) and phospholipase A2(PLA2) were increased by both light irradiation and they were decreased as time flows. And genes associated with ROS releasing, mRNA expressions of tumor necrosis factor receptor (TNFR) and interleukin 1beta(IL1B) were increased by 1 hour red light irradiation but did not by blue light irradiation. As a result, red and blue light irradiation showed different response in affecting the level of ROS. These findings indicate that red light rather than blue light is more useful for anti-inflammation in clinical field
        4,000원
        183.
        2009.02 KCI 등재 구독 인증기관 무료, 개인회원 유료
        The phytochemicals of many plants suggest their potential use as dietary supplements in cancer chemoprevention and chemotherapy. In the present study, antitumor activity of Cudrania tricuspidata, a plant native to East Asia, was investigated. Cell growth inhibition of the extract on HT-29 colorectal adenocarcinoma using MTT colorimetric assay was determined. Apoptosis on HT-29 cells was performed by DNA fragmentation analysis. PGE2 release was measured by enzyme immunoassay, because PGE2 is a key protumorigenic prostanoid in many human cancers. For the ROS scavenging activity, ROS level was detected by laser scanning confocal microscope. It was found that methanol extract of leaves inhibits cell viability by inducing apoptosis as evidenced by DNA fragmentation. Stem bark decreases synthesis of PGE2, inflammatory mediator. Fruits exhibited pronounced ROS scavenging activity. Taken together, these results suggest that Cudrania tricuspidata exerts growth inhibition and anti-oxidation on HT-29 cells through apoptosis, ROS scavenging respectively that it may have anti-cancer properties.
        4,000원
        185.
        2008.08 KCI 등재 구독 인증기관 무료, 개인회원 유료
        In this study, the relationships among obesity, food behavior, and personality traits were studied in male and female elementary school children. The obesity rates were calculated via the measured heights and weights of the subjects. Food behavior was surveyed via a questionnaire. Personality traits were evaluated using an authorized personality test. The obesity rates in the child subjects in this study were 34.1%in the male children, and 28.7% in the female children, and the rates of underweight were 18.2% in the males and 28.7% in the females. The percentage of obese children was slightly higher in the male population than in the female population. With regard to food behavior, the females evidenced significantly higher points than the males, particularly on the items designated 'slow eating' and 'stop eating upon satiety'. Among the personality traits assessed herein, which included emotional stability, general activity, sociability, masculinity, responsibility, reflectiveness, and superiority, the females evidenced significantly higher sociability scores than the males. The personality traits did not appear to be significantly associated with the obesity rate. However, the obese children scored lowest on all personality traits among the three groups. In terms of the relationship between food behavior and personality traits, children with high stability, sociability, responsibility, and superiority also had significantly higher food behavior scores. These results imply that the maintenance of good food behavior may enhance stability, sociability, responsibility, and superiority in children.
        4,000원
        186.
        2008.08 KCI 등재 구독 인증기관 무료, 개인회원 유료
        This study was conducted to determine the quality characteristics of grape jellies for the elderly. The jellies contained sugar (control) or sugar derivative sweeteners (erythritol, isomaltooligosaccharide, sorbitol, and xylitol). Agar (0.31%) and k-carrageenan (0.27%) were the gelling agents. The average age of the subjects participating in the acceptance test was 79. The lightness (L), redness (a), and yellowness (b) values of the agar gel with erythritol mostly decreased, indicating a darker and pale red color. The L and b values of the carrageenan gel with sugar derivative sweeteners increased, indicating brighter and yellowish color. The agar and carrageenan gels with sorbitol showed higher gelling and melting temperature, indicating that gelation occurred easily and did not easily melt. The agar and carrageenan gels with xylitol showed a low-melting temperature, indicating low stability with temperature change. The break-down rate of the agar and carrageenan gels with erythritol was low, whereas that of agar gel with sorbitol was relatively high despite its high melting temperature. Hardness, cohesiveness, gumminess, and chewiness of the gels with sugar derivative sweeteners decreased, and this tendency was most distinct with isomaltooligosaccharide in the agar gel and with sorbitol in the carrageenan gel. The rupture properties of the gel were the same as the hardness of the gel. Sensory acceptance of the agar gels with erythritol, sorbitol, isomaltooligosaccharide, and the carrageenan gel with erythritol was fairly high, whereas that of the agar gel with xylitol and the carrageenan gel with isomaltooligosaccharide and xylitol was low. The results show that sorbitol and erythritol are appropriate as sugar substitutes in grape jellies for the elderly about the acceptability and stability of the gels.
        4,000원
        187.
        2008.08 KCI 등재 구독 인증기관 무료, 개인회원 유료
        본 연구는 설치와 해체가 용이하여 간이식 구조에서 활용되는 시저스 전개형 구조에 관한 것으로, 입면상의 곡률변화 방법을 고찰하고 그에 따른 구조특성을 파악하고자 한 것이다. 먼저 시저스 구조의 입면상 곡률과 시저스부재의 내부절점 편심거리와의 관계를 식과 그래프를 이용하여 나타내었다. 이에 따라, 동일한 시저스부재에서 내부절점 편심거리를 조절하여 곡률을 변화시킬 수 있는 해석모델을 고찰함으로써, 곡률변화에 따른 시저스 구조부재들의 구조적 특성을 고찰하였다. 또한 고찰된 구성방식에 근거하여 실 모델을 제작하고 전개과정을 살펴봄으로써 실제 전개가능성을 검토하였다.
        4,000원
        188.
        2008.03 KCI 등재 구독 인증기관 무료, 개인회원 유료
        시민 건강을 위협하는 질병 및 식중독 오염의 감염 매개체가 되는 손에 대한 위생실태를 점검하고자 유아원, 초등, 중등, 고등학생 및 일반인 등을 대상으로 오염의 지표인 일반세균 및 대장균군과 식중독 원인균인 세균성이질, 살모넬라, 황색포도상구균, 장병원성대장균, V. parahemolyticus, Y. enterocolitica, B. cereus, C. perfringens, L. monocytogenes, C. jejuni에 대해 조사하여 다음과 같은 결과를 얻었다. 1. 일반세균과 대장균군 등의 위생세균에서 대장균군은 유치원생 2명, 초등학생 6명, 성인에서 2명이 검출되었으며, 일반세균은 유치원생의 경우 평균 3.3 log CFU/hand, 초등학생은 3.4 log CFU/hand, 중학생은 3.2 log CFU/hand, 고등학생은 3.4 log CFU/hand, 성인은 3.3 log CFU/hand로 나타났다. 2. 식중독 원인균으로 총 62주(12.4%)가 분리되었는데 그 중에서 황색포도상구균 47주(75.8%), B. cereus 8주(12.9%), C. perfringens 7주(11.3%)로 나타났다. 3. 독소 발현 확인시험은 황색포도상구균의 경우 47주 중 25주(53.2%)에서 독소를 확인하였는데 A형이 22주(88.0%), A와 B형 복합주가 2주(8.0%), A와 C형 복합주가 1주(4.0%)로 나타났으며, B. cereus는 8주 중 7주(87.5%)에서 독소 생산주를 확인하였으나, C. perfringens는 7주 모두 독소를 생산하지 않은 것으로 나타났다. 4. 항생제감수성 시험에서 분리된 황색포도상구균 47주는 ciprofloxacin, clindamycin, imipenem, rimethoprim/sulfamethoxazole, rifampin, vancomycin에 대해서는 100% 감수성을 나타냈으며, cefepime, chloramphenicol, cefotetan, gentamycin은 각각 91.5% 및 oxacillin(89.4%), tetracycline(87.2%)에 대해서도 높은 감수성을 관찰할 수 있었다. 항생제 내성은 ampicillin(91.5%), erythromycin(42.6%) 및 penicillin(95.7%)에서 매우 높게 나타났다. 2개 이상의 항생제 다제내성을 보인 균주는 총 44주였는데, 2제 다제내성 18주(40.9%), 3제 다제내성 15주(34.1%), 4제 다제내성 7주(15.9%), 5제와 6제 다제내성 각각 1주(4.5%)로 나타났다.
        4,200원
        190.
        2008.03 KCI 등재 구독 인증기관 무료, 개인회원 유료
        수수(Sorghum bicolor(L.) Moench) 잡종의 미량(微量) 광물질(鑛物質) 함량에 미치는 정수장(淨水場) 슬러지의 영향에 관해 조사를 했는데, 이번 보고는 니켈 성분의 분석에 관한 것이다. 대조구(對照區), 부숙(腐熟)시킨 정수장 슬러지, 정수장 슬러지+질소-인산-칼륨 비료, 부숙시킨 슬러지+질소, 인산, 칼륨 비료의 4가지를 처리로 하였고, 1, 3, 5시간 예열(豫熱) 시간이 실험 조건이었다. 5시간 이후의 조건에서 백그라운드의 값이
        4,000원
        195.
        2007.06 KCI 등재 구독 인증기관 무료, 개인회원 유료
        본 연구에서는 레일리(순수 기체) 대기 조건 하에서 국내 COMS 연구자들이 사용하는 여덟 개 단파 복사전달모델에서 산출된 네 종류 복사속(flux) 성분을 상호비교함으로써 상대 오차를 조사하였다. 이들 복사속 성분은 지표에서의 직달 일사, 하향 산란, 상향 산란, 그리고 대기 상부에서의 상향 산란이다. 또한 국내 모델의 평가를 위하여, 15개모델을 평균한 Halthore et al.(2005) 결과(예, H15)를 기준값으로 사용하였다. 동일한 태양천정각에서 모델 간의 불일치는 열대 대기에서 수증기에 기인하였고, 한대 대기에서는 오존에 기인하였다. STREAMER를 제외한 국내 7개 모델의 지상에서의 하향 직달일사값은 H15에 대하여 ±4%내에서 일치하였다. 이러한 상대 오차는 태양천정각이 커질 때 증가하였으며, Halthore et al.(2005)에서의 ±3%와 근접하였다. 네 종류 복사속 분석에서 SBDART 모델이 6S 모델에 비하여 전반적으로 우수하였으나, 근적외 파장역에서는 서로 비교할만하였다. 네개 기관의 연구자들이 같은 SBDART 모델에서 산출한 지표에서의 하향 직달일사값 간에도 12.1Wm-2(1.4%)의 불일치가 존재하였다. 불일치의 원인은 부분적으로 복사속 적분에 있어서 서로 다르게 설정된 파장 분해능에도 있었다. 본 연구는 단파 영역에서 최적 모델을 선정하는 데 도움을 줄 수 있다.
        4,500원
        198.
        2007.06 KCI 등재 구독 인증기관 무료, 개인회원 유료
        We have examined the effect of NO donor, S-nitl‘ oso-N-acetyl-DL-penicillamine(SNAP) on heme oxygenase-1 (HQ-l) ex pression in human oral immortalized & malignant keratinocytes, and investigated in the control of keratinocyte proliferation evidence tha t HO-1 cou ld be involved in a low dose of NO, NO inhibitor, HOinducer, and HO inhibitor medi ated cytoprotect ion against cytotoxi city induced by a high dose of NO Oral keratinocyte growth inhibitory or anti-proliferative effects were exerted by with SNAP and hemin in a dose- and cul tivation time dependent manner The level of HQ-1 protein was increased in all cell types after exposure hernin dose, and the hemin induced HQ-1 protein achieved at higher maximum level by 12 hrs in all kind of cells , The pretreatment of cells with 0, 2 μ M SNAP reduced 1 mM SNAP-induced death in IHOK and HN4 cells , These cytoprotective effects on high dose of NO induced HQ-1 expresion and cell ular toxicity were blocked by low dose of SNAP, HCB, and ZnPP IX supporting the involvement of HQ-1 in high dose NO induced growth arrest or cell death, But these cytoprotection pattern is different from immortalized and malignant keratinocytes , These results indirectly demonstrate that HQ-1 could be involved in cytoprotection by NO priming against high dose NO induced cytotoxicity in immortalized and maigla nt oral keratinocytes, Thus, HQ-1 might be an important cellular target of NO donor, with clinical implications for the pre vention of inJlammatory di seases and anti-tumor immunity
        4,000원