Risk assessment traditionally are conducted on individual chemicals; however, humans are exposed to multiple chemicals in daily life. The organophosphotus (OP) pesticides are considered in a single risk assessment because they act by a common mechanism of toxicity, and there is likely to be expose to multiple OP pesticides simultaneously or sequentially. The OP pesticides act by inhibiting the enzyme acetylcholinesterase (AChE) and have available extensive database. AChE is widely distributed droughout the body, most importantly in the nervous system. Inhibition of AChE results in accumulation of acetylcholine in the nervous system that results in clinical signs of cholinergic toxicity, including increased salivation and lacrimation, nausea and vomiting, muscle fasciculation, lethargy and fatigue, among others. To conduct an exposure assessment for pesticides in the diet, we need to know the food consumption patterns of the population, and the pesticide residue levels in the foods that are consumed. This study was conducted to identify cumulative dietary risk due to multiple OP pesticides that can be exposed through various foods. Total 22 food samples including cereals, vegetables and fruits were collected randomly two times from food markets in several sites (4 cities). The subjected foods were selected by regarding of highly consumed foods to general Korean people. The 12 OP pesticides including Acephate, Azinphos-methyl, Chlorpyrifos, and Diazinon were monitored. For the exposure assessment, general adult group of 60 kg body weight was regarded as target population and food consumption data suggested by Lee et al. (2000) were used as consumed value of individual food Analyses of samples for OP pesticides have been carried out according to the multiclass multiresidue analysis method and acephate and methamidophos analysis method of Korea Food Code. In general the levels of OP pesticides found in the food samples were very low or not detected. The detected highest value was 0.282 ppm as methidathion in mandarin and acephate, chlorpyrifos, methamidophos and methidathion were detected in several foods. To quantify simultaneous exposure, toxic equivalency factor of proportionately corresponding potency giving decreased AChE activity on the basis of reference dose 0.3 ㎍/kg/day to the chlorpyrifos was applied. The estimated dietary daily intake of OP pesticide was 4.52 × 10^(-2) ㎍/kg/day as mean value. The hazard index (HI) is the sum for OP pesticides being considered together was 0.15. The HI less than 1 indicates the exposure may be lower than an acceptable level.

본 연구는 상품화된 육수 재료의 이용 현황 및 소비자 의견을 조사하기 위하여 수행되었고 이를 위해 서울에 거주하는 500명의 주부를 대상으로 설문조사를 실시하였다. 그 결과, 사용하는 제품의 종류는 분말형이 가장 많았고 시간 절약과 편리함이 상품화된 육수재료를 사용하는 가장 주된 이유였다. 또한 육수 재료를 사용하는 방법으로는 육류로 만든 육수에 섞어서 사용하는 방법이 가장 많이 쓰이고 있었다. 상품화된 육수 재료의 기호도에 대해서는 응답자의 81%가 상품화된 육수에 대해 '좋아하지 않는다'라고 하였다. 좋아하지 않는 가장 큰 이유는 '첨가제에 대한 염려와 인공조미료 때문'이었다. 연령이 높고 결혼지속년수가 길수록 상품화된 육수 재료를 좋아하지 않는 비율은 높아졌다. 상품화된 육수가 품질만 보장된다면 응답자의 75%는 사용할 의사가 있는 것으로 나타났으나 나머지는 아무리 좋은 품질이라 해도 육수는 직접 제조하겠다고 응답하였다. 60대 이상의 응답자들만은 상품화된 육수를 사용하지 않겠다는 비율이 더 높아 60대 이상의 고령층은 상품화된 육수 제품에 대해 가장 거부감이 큰 연령대인 것으로 판단된다. 본 연구 결과, 상품화된 육수의 수요는 크나 시판되는 제품들에 대한 만족도는 낮은 것으로 나타났으므로 소비자 요구를 반영한 육수 제품에 대한 연구가 시급한 것으로 생각된다.

The purposes of this study were to investigate changes in serum lipid levels with age and gender, and to determine which factors affect the serum lipid profiles. The anthropometric parameters(height, weight, waist girth, hip girth) and biochemical status(cholesterol, triglyceride, HDL-chol.) were measured for clinically normal adults(male 89, female 91) in Yeongdong area. The results are as follows: 1. The obesity index was significantly higher in female(115.2±15.2%) than in male(109.9±13.4%), but waist/hip girth ratio(WHR) was significantly higher in male (0.89±0.05) than in female(0.81±0.06). 2. Male subjects had higher triglyceride and atherogenic index and lower HDL-cholesterol and relative cholesterol than those of female subjects. 3. Prevalences of hypercholesteolemia, hypertriglyceridemia and hypoHDL-cholesterolemia were 9.0%, 9.0%, 14.6% respectively in male and 9.9%, 2.2%, 4.4% in female. 4. WHR positively correlated with serum cholesterol, TG, LDL/HDL and A.I., and negatively correlated with HDL-chol. and relative chol. Correlation analyses indicated that WHR seemed to be more closely associated with serum lipid levels(rather than obesity index). 5. Age showed positive correlations with waist girth, WHR, cholesterol, LDL-cholesterol, LDL/HDL and A.I., but negative correlations with height, body weight and relative cholesterol. 6. There was significant differences in TG concentration between drinker(169.3±130.0mg/dl) and non-drinker(111.4±64.5mg/dl), and smoker(165±103.6mg/dl) and non-smoker (110.8±39.0mg/dl). That is to say that as risk factors for hyperlipidemia are obesity index, serum lipid concentration, life style(such as alcohol drinking and smoking) and age. Specially major risk factors are drinking, smoking and regulated exercise in male and age is an important risk factor in female.

This study was examined the current situation of utilization of meat broth materials for the development of various broth products. The 500 housewives living in Seoul were asked. The results obtained as follows; Beef(93%) was used most frequently for making broth. In order to reduce rancid smell of broth, garlic(72%), ginger(68%), green onion(60%), onion(58%), black pepper(44%), radish(25%) were used. Most housewives used beef three or four times a month and hardly used chicken or pork for making broth. Commercial broth materials were used two or three times a month. For making broth the bricket of beef, a whole or half chicken and bones of pig were consumed most frequently. Before making broth, from flesh only blood was removed. Also housewives slightly boiled bone blood-removed in water. The older age is, the longer marriage duration is, the higher monthly income and education level is, the more beef was consumed. The higher the education level is, the more vegetables like garlic, ginger, green onion, onion, radish were used. It is suggested that in order to develop various broth products made of natural materials, the characteristics of preferred broth materials should be defined and it is necesarry to accomplish the preference test of preferred broth products.

뇌전위에서 개인차가 없는 일반적인 규칙을 지닌 두개의 정보 변수, 즉 ILF와 IHF를 발견하였다. 이러한 일반성을 지닌 정보 변수가 청각, 후각, 촉각 자극에 의해 유발된 쾌하거나 불쾌한 감성 상태를 구분할 수 있으며 전두엽에서 그 경향이 두드러짐을 확인하였다. 전두엽의 뇌전위에서 감성 자극이 주어지가 전과 자극이 주어지는 동안의 ILF, IHF값을 정규화함으로써 새로운 변수, Relative Quantified Emotional State(RQES)를 구현하였다. RQES는 쾌, 중립, 불쾌한 감성의 정도를 선형적으로 정량화하였다. 따라서 하나의 전극으로 측정한 전두엽부분의 뇌전위로부터 RQES값을 계산하면 인간의 쾌, 불쾌 감성을 신뢰도있게 정량화 할 수 있다.

Consumption of edible oil at food service institutions in Inchon was surveyed to provide basic data for continuous education of dietitians. Manufacturing industry was the major food service institution(78.8%) in Inchon followed by schools, hospitals and social welfare service centers. Most dietitians were at the age of twenties and college graduates with professional careers of 1-5 years. Oil was purchased on the basis of its quality within 1-3% of total food costs four times a month. Proper frying temperature was determined by dropping salt or food coating materials into the oil. Soybean oil was the most frequently used and commercial frying mix powder and flour and eggs in water were the most common food coating materials. Fish and commercial frozen foods were the most frequently used materials for frying. Fried foods were stored with covers in a basket and consumed within 30 minutes after cooking. Frying oils were used one more time after filtering and color was the index for determination of re-use.

호열균 B. stearolhermophilus의 세포내 PPIase를 정제하여 Edman법으로 N-말단 아미노산 배열을 결정하여 이를 바탕으로 합성한 올리고누클레오티드의 프리머를 이용하여, 서턴 분석하여 PPIase 유전자 약 3.0kb를 클로닝하였다 (pPI-40). PPI-40으로부터 PPIase N-말단 배열을 코드하는 영역으로부터 합성한 프리머(A-1, B-2)를 이용하여, PCR법으로 PPIase N-말단을 코드하는 유전자를 증폭하여, 염기배열을 결정한 후, 그 정보에 따라 유전해석한 결과 PCR로 증폭된 단편(pSN-18)은 165염기로부터 형성된 55 아미노산잔기를 코드하는 open reading frame (ORF)이 계속되고 있었고, Edman법으로 결정한 PPIase N-말단 아미노산 39 아미노산잔기가 완전히 일치하였다. 이 결과로부터 pSN-18의 ORF은 PPIase 구조 유전자의 약1/3에 해당하는 단편임을 확인하였다. 그리고, 이 ORF를 중심으로, 지금까지 클론화된 대장균의 PPIase (cytoplasm)와 PPIase b(periplasm)의 아미노산 일차구조 해석으로부터 각각 58%(cytoplasm), 16% (periplasm)의 상동성을 나타냈다. PPIase 구조 유전자를 갖는 재조합플라스미드 pPI-40을 JM109로 형질전환하여 Lac 프로모터로 PPIase 단백질을 발현시켰다. 효소 분자량을 SDS-PAGE로 확인한 결과 약 18kDa으로 호열균 B. stearolhermophilus로부터 정제한 단백질 분자량과 동일하다.면역억제(CsA, FK506)와의 화학적인 반응은 대장균의 PPIase와 동일하게, 면역억제와는 비감수성으로 나타났다.

The purpose of this study was to evaluate the foodservice management practices in child-care centers in order to provide basic information for the development of a model of a centralized food service information center. This approach was achieved using a variety of qualitative and quantitative information including general foodservice management practices and plate waste. A self-completed questionnaire survey of 651 child-care centers in Korea was undertaken and detailed information was carefully collected at 6 representative child-care centers. The results of the empirical survey were as follows: 1. Child-care centers categorized by location were in large cities (59.9%), medium cities (27.6%) and in provincial areas (12.5). 2. Private sector of child-care centers was 46.4% of the total followed by National/public (44.2%) and licensed home day-care programs (9.4%). 3. Total average number of children in child-care centers was 63.3±43.1 with a very significant difference (p〈0.001) in types of child-care centers. 4. The average space of kitchen and dining room was 5.0±3.8 and 10.8±11.0 pyung (1pyung=3.3058 cm2). 5. The average cost of interim snack in morning and afternoon in child-care centers were 345.9±459.3 won and 359.3±226.6 won respectively. The average cost of lunch was 644.0±481.1 won. There was a significant difference (p〈0.001) by types of child-care centers with a highest cost of 863.9 won in licensed home day-care programs. 6. Only a limited number of dietitian were employed, therefore most of food service management practice was not conducted by professional personnel. 7. The result of nutritional analysis of the food revealed that the level of energy and nutrients contained in the food was below the recommeded level (RDA/3).

Sprague-Dawley 흰쥐에 고지방식이로 6주간 식이하면서 천궁 열추출액을 경구투여하여 혈장, 간장 및 부고환조직에서 지방성분, 지단백질의 분포, 변중의 담즙산과 중성스테롤들의 배출량을 측정하여 다음과 같은 결과를 얻었다. 고지방식이와 천궁(CR군)열수추출액을 각각 투여한 흰쥐의 혈청중 총콜레스테롤, 중성지질 및 유리지방산의 농도는 고지방식이로만 사육한 군(CON군)에 비해 낮았다. 한편 인지질과 HDL-cholesterol의 농도는 다소 높았다. 또한 간장과 부고환의 지방성분들을 조사한 경우에도 위와 같은 결과를 얻었다. 고지방식이가 급여되는 동안 정상군보다 대조군에서 low density lipoprotein(LDL)과 very low density lipoprotein(VLDL)이 다같이 증가하고 high density lipoprotein(HDL)의 농도는 감소하였다. 천궁투여군에서는 대조군에 비하여 LDL, VLDL이 감소하였고 HDL은 증가하였다. 그리고 천궁투여군은 대조군에 비하여 분변을 통한 담즙산과 중성스테롤의 배설량이 증가하였다. 이상의 결과를 종합해 보면 천궁투여군이 지방축적 및 고지혈증의 완만한 개선경향을 나타내었다.

호박의 지방산 조성에 관하여 gas chromatography법으로 분리 정량하여 다음과 같은 결과를 얻었다. 호박의 일반성분은 수분 57.3%, 탄수화물 40%, 조단백질 2.2%, 조지방 0.1%, 조회분 0.4%이었다. 지방산은 15종이 검출되었고, 호박의 성장일수에 따른 지방산 조성의 변화는 C_14:0, C_15:0, C_16:1, C_17:0, C_18:0, C_18:1등은 20일 경과된 때가 많았으나 시간이 지남에 따라 점점 감소하는 경향을 보였으며, C_20:2, C_20:3, C_20:0은 어린 호박에는 검출되지 않았다. 그리고 C_18:3인 경우는 완전히 노랗게 익은 것이 어린 것일 때보다 90% 정도 증가하였다. C_16:0의 경우는 C(22.5%), B(22.9%), A(26.6%)를 보여 커짐에 따라 증가되었으며, C_16:1와 C_18:1의 경우는 A가 가장 적고 C가 가장 높았다. Docosahexaenoic acid와 eicosapentaenoic acid의 전구체인 ω-3 fatty acids인 α-linoleic acid가 44.8%로 가장 많았으며, linoleic acid(17.2%), stearic acid(3.5%) 등이었다. 그리고 polyunsaturated fatty acid가 62.2%이었고, n-6은 17.4%이었으며, n-3/n-6 ratio는 2.6이었다.

In this report, we investigated meal patterns by area, age, profession from the data of the Korean National Nutrition Survey which had been carried out in 1989. The proportion of home prepared meal was about 90% at breakfast and dinner, and 46.3% at lunch. People had meals outside of home mainly at lunch(31.1%), and usually ate restaurant meal(17.7%). The proportion of meal skipped was 7.1% at breakfast, 3.9% at lunch, and 1.2% at dinner. At large city, 10.3% of people skipped breakfast, it was the highest proportion among three areas(large city, small city, and rural area). Also, the proportion of meal outside of homes was the highest among all areas. In rural area, home prepared meal was prevalent for all meals. Among all age groups, people between 20 and 49 ate out most frequently. For people between age 16 and 19, meal skipping was frequent for breakfast and dinner, especially, 20.5% of girl skipped breakfast. At lunch, the proportion of meal skipping was the highest in age higher than 50 groups. Among all professions, people in service job skipped lunch and dinner at the highest proportion. Manager and deskworker skipped breakfast by 12.9% and they ate out at lunch by 82.5%.

서울시(市)의 역삼동 강남역 부근, 잠원동, 남대문시장(南大門市場) 등 세군데서 영업(營業)하는 포장마차(布張馬車)를 대상(對象)으로하여 1987년(年) 6월(月) 25일부(日)터 1987년(年) 8월(月) 25일(日)까지 영업(營業) 및 위생(衛生)과 고객(顧客)에 관한 실태(實態)를 조사(調査)한 결과(結果)는 아래와 같다. 포장마차(布張馬車)는 주로 30~40대(代) 여자(女子)가 종사(從事)하는 경우가 많았으며 1~2명(名)이 영업(營業)하는 형태(型態)가 가장 많았다. 포장마차영업자(布張馬車營業者)는 3년(年) 이상(以上) 한 사람이 거의 60%였으며 가장 큰 문제점은 자녀(子女)의 교육(敎育)문제, 단속(團束), 수면불족(睡眠不足)으로 들었고, 영업(營業)에 종사(從事)하는 만족도(滿足度)는 저조(低調)하였다. 포장마차(布張馬車)의 영업실태(營業實態)를 보면 식품재료(食品材料)의 구입(購入)은 소매시장(小賣市場)에서 직접 구입하였고, 팔다남은 재료(材料)는 보관후 다시 사용하는 경우가 많았다. 포장마차(布張馬車)에서 판매(販賣)하는 음식은 주류 및 안주와 간식이 주종이었는데, 각 지역(地域)에서 10위(位)안에 드는 음식은 소주와 김밥이었고, 대체로 구이형태의 음식이 많았으며 실내형(室內型)은 주류 및 안주형이 많았고 이동형(移動型)은 간식을 많이 팔고 있었다. 포장마차(布張馬車)의 총 영업시간(營業時間)은 오후 2시경~밤 0시까지 약 10시간(時間) 정도였고 1일(日) 매상액(賣上額)은 이동형(移動型)은 2-3만원, 실내형(室內型)은 4-5만원 정도였고 포장마차(布張馬車)의 약 60% 정도가 자리세를 물고 있었다. 포장마차(布張馬車)의 시설면에서 연료는 주로 연탄을 사용했고, 조명은 전기 혹은 밧데리를 사용했으며 상수도시설은 실내형(室內型)일 경우만 갖추고 있었고 이동형(移動型)은 급수 및 배수가 어려워 위생상태(衛生狀態)가 염려되었다. 위생상태(衛生狀態)는 부합리적(不合理的)인 플라스틱제품의 식기(食器)의 사용, 급수·배수의 어려움, 비위생적(非衛生的)인 세척방법, 재료(材料)의 비위생적(非衛生的) 보관방법, 먹다남은 음식의 재사용 등 포장마차(布張馬車)의 위생실태(衛生實態)는 개선(改善)되어야 할 점이 많이 나타났다. 포장마차(布張馬車)를 주로 찾는 고객(顧客)은 20~40대(代)의 남자(男子)로 직장인(職場人)과 대학생(大學生)이 많았으며 위치에 따라 이용하는 고객(顧客)의 부류가 조금씩 달랐으며 포장마차(布張馬車)를 찾는 동기(動機)는 부담없고 자유(自由)스러운 분위기 때문에 찾는 경우가 많았다.

To determine dining out behaviours of fast foods at five different fast food restaurants of Youido apartment compound in Seoul, a survey was conducted of 460 customers ranging in age from 7 to 29 year, from October 1 to 5th, 1986. Findings are summarized follows: The reasons the customers the fast food restaurants by the highest percentage were the following in the order: 'the atmosphere in which fast food is eaten is enjoyable for the companionship', 'convenient to dining', 'the surroundings and dining equipment are pleasant and hygienic', and 'to be able to stay as long as I want'. The majority of the customers visited the fast food restaurants with their friends between 1pm and 5pm. The fast foods purchased by the customers were mostly for snack rather than a full meal. Preference was a major factor in food selection from available meun items. Ice cream, Kentucky fried chicken, rolled rice with laver I rice cake stew, fried Mandoo were ranged high on the list of liked foods, in contrast, lower preference was for noodle soup, small red bean porridge, pinenut porridge, and persimmon punch.

This study had two main objectives. We first investigated which weather phenomena people were most concerned about in the context of climate change or global warming. Then, we conducted content analysis to find which words were more commonly used with climate change or global warming. For this, we collected web data from Twitter, Naver, and Daum from April to October 2019 in the Republic of Korea. The results suggested that people were more concerned about air quality, followed by typhoons and heat waves. Because this study only considered one warm period in the year of 2019, winter-related weather phenomena such as cold wave and snowfall were not well captured. From Twitter, we were able to find wider range of terminologies and thoughts/opinions than Naver and Daum. Also, more life-relevant weather events such as typhoons and heat waves in Twitter were commonly mentioned compared to Naver and Daum. On the other hand, the comments from Naver and Daum showed relatively narrower and limited terms and thoughts/ opinions. Especially, most of the comments were influenced by headlines of articles. We found many comments about air quality and energy/economic policy. We hope this paper could provide background information about how to promote the climate change education and public awareness and how to efficiently interact with general audiences.

All of the animals and the plants in ecosystem are intimately connected to one another and the changes of forests and surroundings affect directly wild animals. This study was conducted at Hangyeong-myeon Cheongsu-ri located in the western part of Jeju Island belonging to Hangyeong·Andeok Gotjawal Zone and Jocheon-eup Seonheul-ri located in the eastern part of Jeju Island belonging to Jocheon·Hamdeok Gotjawal Zone. The survey on advent of birds was carried out twice a month from January 2014 to December 2015. We divided habitat environments into three survey sites such as a forest, a shrub forest and a farmland. A total of 65 species and 4,802 individuals were observed during the survey period. In a forest, 36 species and 1,287 individuals were observed while A shrub forest had 40 species and 1,554 individuals. And in a farmland, 41 species and 1,961 individuals were observed. The only 10 species were observed in forest and the only 7 species in shrub forest and the only 10 species in farmland. The species diversity and the evenness of a farmland were the highest, and the species richness was the highest in a shrub forest, and the dominance of a forest was the highest among the three areas. The similarity index between a shurb forest and a farmland was high while that between a forest and a farmland was low. The similarity index related with breeding appeared that a forest and a farmland was different from each other.

Cell based grid data of future temperature and precipitation produced with four RCP scenarios were converted into polygon based data for administrative districts using three simple vectorizing methods; (1) KMA Dong-Nae forecast point based, (2) areal ratio based and (3) central point based methods. The results were compared the existed KMA areal weight based methods to identify which methods were more efficient than others. Simple statistical methods such descriptive statistics, correlation coefficient, and Bland & Altman plots (B&A) were used to compare agreements between them. When central point and areal ratio based methods were applied to administrative districts of Eup-Myeon-Dong or some Gus, NULLs were found because their sizes are smaller than the cell of 1x1 km. Therefore, KMA Dong-Nae forecast point based methods were better when sizes of administrative districts are smaller than the cell size. For Do and Metropolitan cities, there were no greater differences among methods except for the KMA Dong- Nae forecast points. The greater the areas of administrative districts the more distortions from the KMA Dong-Nae forecast points because only KMA Dong-Nae forecast one point were used for the calculation. In conclusion, the KMA Dong-Nae forecast point based method was appropriate when sizes of administrative districts are smaller than the grid cell. For the greater areal sizes such as Do and Metropolitan cities, areal ratio and central point based methods were better.

This study was carried out to investigate the vascular plants of Mt. Choejeong in Gachang-myeon, Daegu. From March 2017 to October 2018, a total of 22 studies were conducted. The vascular plants surveyed were grouped into 560 taxa, including 104 families, 297 genera, 495 species, 4 subspecies, 51 varieties and 10 forma. Endemic plants 15 taxa, Rare plants 5 taxa, Red list plants 5 taxa, Floristic regional indicator plants 54 taxa, Naturalized plants 36 taxa were recorded. Among surveyed 560 taxa, edible, medicinal, ornamental, timber, pasturing, industrial and fiber plants included 246 taxa (29.2%), 228 taxa (27.1%), 164 taxa (19.5%), 61 taxa (7.2%), 13 taxa (1.5%), and 8 taxa (0.9%). And because people are coming and going more frequently than in the past, this will result in more frequent influx of naturalized plants and a threat to the habitat of the plants that are currently growing.

본 연구는 전라남도 여수시 돌산읍에 위치하는 돌산도의 관속식물상을 조사하고 분포가 확인된 주요 식물에 대해 논의하였다. 현지조사는 2017년부터 2018년까지 총 15회에 걸쳐 수행되었으며, 이에 대한 증거표본은 국립수목원 산림생물표본관(KH)에 소장하였다. 분포가 확인된 관속식물은 113과 338속 517종 1아종 49변종 3품종 총 570분류군이다. 이중 한국특산식물은 개족도리풀 등 18분류군을 확인되었으며, 환경부지정 식물구계학적특정식물종은 V-I등급으로 총 90분류군이 조사되었다. 희귀식물은 위기종(EN)인 백양꽃 1분류군을 포함하여 총 4분류군이며, 적색목록에 속하는 관속식물은 취약종(VU) 갯취 등 총 8분류군이다.