This study constructed a wheat-specific primer and a probe using the internal transcribed spacer (ITS). 2 regions of wheat (Triticum aestivum) and real-time PCR conditions were established. The calibration curve showed a slope of -3.356, a correlation coefficient of 0.998, and an amplification efficiency of 98.589%. Experiments were carried out on the rice flour mixed with 50%, 10%, 1%, 0.1%, 0.01%, and 0.001% of wheat. The result showed that it was possible to detect samples mixed with up to 0.01% of wheat. As a result of checking, the wheat detection potential of rice, 34 processed foods, and seven processed foods was ascertained. The real-time PCR method using the wheatspecific primer and probe developed in this study can be used to identify the authenticity of the raw materials, such as the incorrect indication of the raw materials utilized and the unintended mixing of wheat during the manufacturing process.

이 연구의 주 목적은 친환경농산물가공식품 구입경험이 있는 소비자의 개별 특성과 이 식품의 소비확 대 정책 선호 간 상관관계를 분석하는 데 있다. 연구의 목적을 달성하기 위해 소비자를 대상으로 친환 경농산물 가공식품 소비실태를 조사하였고, 실증 분석을 위해 다항로짓모형(Multinominal logit model) 을 활용하였다. 분석결과는 인증제도 관리가 강화될 때 소비를 늘릴 의향이 있는 소비자는 인증마크를 확인하는 응답자, 상대적으로 건강에 유익할 것으로 인식하는 응답자들인 것으로 나타났다. 가격이 인하될 때 소비를 늘릴 의향이 있는 소비자는 친환경가공식품 가격이 높다고 생각하는 응답자들, 친환경 가공식품의 안전성에 만족하는 응답자들인 것으로 나타났다. 친환경농산물 가공식품 구매를 효과적으로 확대시키기 위해서는 이러한 소비자의 개별특성을 고려하여 맞춤형으로 정책을 추진하는 것은 유용할 것이다.

This study aimed to develop processed foods that can be tailored to the tastes of consumers in countries to enter domestic and foreign markets utilizing fresh Korean pears, in which the consumption is decreasing. A survey was also conducted on three types of samples (pear jelly, pear rice cake, and pear muffin). As a result, both Korean and Chinese women aged in their 20s preferred pear muffins the most among the pear products evaluated. Pear jelly and rice cake were preferred by Chinese consumers because of their sweet taste (p<0.05). Pear rice cakes were preferred because of their texture (p<0.05). Pear muffins were not significant in all items except for odor/flavor and sweetness, but Korean consumers had a high preference for them and showed a significant preference for colors (p<0.05). Pear muffins were most familiar to both Korean and Chinese consumers showing a high willingness to purchase. An analysis of the preference inducement factors of consumers in each country of the three processed foods containing pears using Check-All-That-Reply (CATA) showed that the consumers of both countries preferred the ‘pear odor/flavor’ characteristics of pear jelly, and that pear rice cakes were preferred by Chinese consumers compared to Korean consumers. Pear muffins were preferred by Korean consumers. Overall, pear muffins are the product expected to be most suitable for female consumers in Korea and China aged in their 20s.

In this study, hardness, which is one of the most important texture properties of meat products, was investigated in order to evaluate the potential of using crushed meat products as food for seniors. The hardness of crushed meat products in the Korean market, such as hamburg steak (12 products), meatball (8 products), ddeokgalbi (rib patties, 13 products), and wanja (meat dumplings, 10 products) were analyzed through a texture analyzer. Also, the hardness of UDF 1st grade hamburg steak (Japanese care food) was assessed in comparison with the texture characteristics of crushed meat products. The mean value of hardness of 43 crushed meat products purchased in the Korean market was 64,733±12,319 N/m2 (23,650-102,780 N/m2) while the hardness of UDF 1st grade product was 50,910±4,990 N/m2. The hardness of 16 crushed meat products was not significantly different from that of UDF 1st grade products (p<0.05). On the other hand, crushed meat products had higher relative standard deviation (RSD, %) values of hardness (mean: 19.6%, from 7.8 to 43.1%) than the RSD of UDF 1st grade products (9.8%) except for three products. Therefore, crushed meat products could be a developable “food for seniors” if deviations of texture are minimized. These findings on texture properties could be an effective data base for the development and quality control of “food for seniors” using meat materials.

가공식품에서 폴리아크릴산나트륨에 대한 분석 방법을 size-exclusion chromatography를 사용하여 개발하고 유통중인 가공식품에 대해 모니터링 하였다. 분석조건에 대하여 GF-7M HQ column과 UV / VIS 검출기를 피크 모양과 선형성을 기준으로 선택하였으며, 유속, column oven 온도 및 이동상은 각각 0.6 mL/min, 45℃ 및 50 mM sodium phosphate buffer (pH 9.0)으로 선정되었다. 시료의 전처리를 위하여 pH 7.0의 50 mM sodium phosphate buffer로 20℃, 150 rpm에서 3 시간 동안 추출하였다. 확립된 분석법을 검증한 결과 적절한 선택성을 나타내었으며, 검량선은 50~500 mg/L의 범위에서 선정되었고, 이때 검량선의 상관계수(R2)는 0.9985로 나타났다. 폴리아크릴산나트륨의 검출한계(LOD)는 10.95 mg/kg으로 정량한계(LOQ)는 33.19mg/kg의 값을 얻었으며, 정밀도는 intra-day의 경우 3.0~8.3%를, inter day의 경우 1.3~2.6%로 확인되었다. 정확도는 intra-day의 경우 99.6~127.6%를 나타내었으며, interday의 경우 94.3~121.9%로 확인되었다. 유통 중인 가공식품 125품목을 대상으로 폴리아크릴산나트륨의 함유량을 분석한 결과 40품목에서 검출되었으며, 검출량은 식품첨가물공전의 규격인 0.2% 미만으로 검출되었다. 본 연구 결과는 size-exclusion chromatography에 의한 분석방법이 가공식품에서 폴리아크릴산나트륨을 분석하는데 적용할 수 있는 적합한 방법이라는 것을 나타내었다.

The purpose of this study was to examine the extent of purchase of processed foods and the awareness about food label among middle school students. This survey was conducted by involving 350 middle school students in Incheon city, Korea from June 17~19, 2015. Middle school students consumed confectioneries, bread, carbonated drinks, and juices and ice creams once or twice a week at the rate of 53.4, 53.0, 40.6, and 36.9%, respectively. The most frequent place, time, and reason to purchase the processed foods were ‘convenience store (36.2%)’, ‘after school (26.8%)’, and ‘hunger (77.9%)’. The subjects exhibited top priority (57.0%) on the taste at the time of purchasing the processed foods. Interestingly, the girl-students (44.7%) checked the labels of food more than the boy students (34.0%). The reasons for checking the food labels included acquiring significant information about the shelf life (27.0%), price (18.1%), nutrient (19.1%), and food additives (14.1%). Among the food labeling information, the name of the product (55.7%), the date of manufacture (49.3%) and the content (32.6%) were checked mainly by the subjects. In addition, the major reason for not confirming the food labeling was ‘the food label was too small or crude (31.9%)’. It is necessary to inform about the processing methods and ingredients of the processed to middle school students so that they can make the correct choice of processed foods. Development of proper education methods on nutrition for middle school students is necessitated for healthy living.

The purpose of this study was to develop an indirect enzyme-linked immunosorbent assay (indirect ELISA) based on a monoclonal antibody (MAb) that is specific to mackerel thermal stable-soluble protein (TSSP), that can be used for the rapid detection of mackerel in processed marine foods. Among the four MAbs (3A5-1, 2, 9, and 12) developed in previous studies, the 3A5-2 MAb that showed high specificity and sensitivity were selected and used to develop the indirect ELISA method. The detection range of the indirect ELISA was 0.02%-0.001% and the detection limit of 0.001% was shown. No cross-reaction to other marine products and food ingredients was observed by the indirect ELISA. Processed marine foods containing mackerel with ≥ 0.3 O.D. value at 405 nm were estimated as positive samples by the indirect ELISA. Therefore, the indirect ELISA can be used as a rapid and sensitive method to identify mackerel authenticity and adulteration in processed marine foods.

This study evaluated how fresh pear cognition variables influences the purchase of primary and secondary products to promote pear consumption. The survey was performed online using 200 consumers who have consumed pears. Consumers’ cognition was classified into two groups, one that was more favorable toward fresh pears and one that was less favorable. The cognition toward pear significantly affected the attitude (p<0.001) and purchase intention (p<0.001) toward fresh pear. When consumers bought fresh pear, sweetness was most important among the selection attributes to both groups, and the most common reason why consumers liked the pear analyzed by check all that apply (CATA) was also sweet taste. However, the negative group did not prefer fresh pear because of taste and texture, so those qualities should be wellcontrolled. In addition, cognition toward primary products influenced liking and purchase intention of processed pear products, especially beverages (p<0.001), bakeries and sweets (p<0.05). Therefore, these types of secondary products should be developed based on consumers’ demands.

The objective of this study was to increase the efficiency of starch extraction from potato sludge by different concentration of food-grade hemicellulase. The potato sludge, which is a by-product of potato processing industry, was treated with food-grade hemicellulase. Starch extraction efficiency displayed no significant difference in hemicellulase concentration. The purities of potato starch increased from 83.40 to 95.91, 97.44, 95.58, and 97.79%, with treated 0.5, 0.75, 1.0, and 1.5% hemicellulase, respectively. The physicochemical properties of the starches, such as granule structure, particle size, pasting, and thermal transition, were not affected by the concentration of hemicellulase. These results indicate that food-grade hemicellulase treatment is an efficient method for starch extraction from potato sludge.