본 연구는 초미세분쇄기술을 이용하여 옥수수전분의 입자구조파괴가 이루어졌을 때 분자구조적, 물리적 변화가 어떻게 이루어지는지를 구명하고자 하였다. 초미세 분쇄처리 후 옥수수전분의 평균직경은 약 50% 감소가 이루어졌으며 비표면적은 567% 증가하였다. 초미세분쇄처리 전·후의 옥수수전분에 대한 분자량분포를 GPC로 측정한 결과 분쇄 후 저분자량의 Peak II의 면적이 21.0%에서 86.5%로 상승하였다. 손상전분 함량은 각각 9.63%와 83.57%로 초미세분쇄처리에 의하여 크게 증가하였다. 옥수수전분의 경우는 겔(gel)을 형성하였으나 초미세분쇄처리 후에는 전분의 분쇄과정에서 전분입자파괴와 아울러 옥수수전분의 분자량이 저분자화 되면서 겔 형성능력이 크게 저하되었다.

This study was conducted to investigate the quality characteristics of mungbean starch gels containing various hydrocolloids (carrageenan, locust bean gum and xanthan gum) during room temperature storage (25℃ for 24, 48 and 72 hours). Carrageenan and xanthan gum reduced the pasting viscosity of mungbean starch, whereas the locust bean gum increased the viscosity. The melting characteristics, as assessed by DSC, showed that carrageenan and xanthan gum delayed gelatinization of mungbean starch and the locust bean gum had no effect on this property. The lightness (L) of the gels with the locust bean gum was similar to that without the additive during storage, whereas that with carrageenan and xanthan gum was higher than that without the additive. Hardness, chewiness and gumminess of the gels with the locust bean gum was higher than that without the additive during storage, whereas that with carrageenan and xanthan gum was lower than that without the additive. The rupture stress, rupture strain and rupture energy of the gels with carrageenan and xanthan gum was lower than that without the additive during storage, whereas that with the locust bean gum was similar to that without the additive. In the sensory evaluation, springiness and cohesiveness of the gels with carrageenan and xanthan gum were lower than those without the additive, whereas springiness, brittleness and hardness of the gels with the locust bean gum were higher than those without the additive. In addition, the overall acceptability of the gels with the locust bean gum improved. The above results showed that carrageenan and xanthan gum lowered the quality characteristics of the mungbean starch gel and the locust bean gum improved them. Thus, the addition of 0.5% locust bean gum is an appropriate method for improving the quality characteristics of mungbean starch gel.

Male weanling Sprague Dawley rats were used to evaluate the effect of dietary rice starch with different particle size on growth performance, intestinal function and proliferation. There were two dietary treatment: rice starch (RS), ultra finely pulverized rice starch with less than 15μm size (PRS). They were eight rats per treatment. In vitro digestibility, body weight change and organs weight were evaluated. Serum GPT, GOT and blood urea nitrogen were analyzed. Transit time, short chain fatty acid contents of cecum, and cell proliferation of duodenum and jejunum were measured. In vitro digestibility of PRS was higher than that of RS. Rats fed ultra finely pulverized rice starch for 3 weeks grew faster than rats fed rice starch. PRS group has higher weights of liver, kidney, spleen and epididymal fat pad, perhaps as a result of increased digestibility. GPT and GOT were not different between two groups. Blood urea nitrogen was higher in RS-fed rats than that of PRS-fed rats. Feeding ultra finely pulverized rice starch resulted in a proliferation of duodenum significantly. These results suggest that ultra finely pulverized rice starch increases the growth performance in weanling animals with reduced number of cells in the cell cycle of small intestine.

본 연구에서는 난소화성 전분을 0%, 10%, 20% 및 30% 수준으로 대체한 슈거쿠키를 제조하여 이화학적 검사와 관능검사 그리고 기호도 검사를 통하여 품질 특성을 조사하였다. 반죽의 pH는 난소화성 전분을 30% 대체한 반죽이 다른 시료군에 비해 유의적으로 높은 pH를 나타내었다. 반죽의 수분함량은 대조군이 유의적으로 높은 수분함량을 나타내었고 난소화성 전분의 대체비율이 높아짐에 따라 유의적으로 낮은 결과를 나타내었다. 쿠키의 퍼짐성은 난소화성 전분의 대체비율이 높아짐에 따라 유의적으로 높게 평가되었다. 30% 시료군은 다른 시료군들에 비해 유의적으로 높은 L값과 a값을 나타내었다. 난소화성 전분을 30% 대체한 시료군은 높은 보습력으로 인하여 퍼짐성 특성이 높게 평가되었고 또한 명도와 적색도가 높은 쿠키로 평가되었다. 쿠키의 관능검사 결과 30% 시료군은 다른 시료군들에 비해 경도와 표면에 금이 갈라진 정도가 유의적으로 약하게 평가되었다. 쿠키의 밝은정도에서는 30% 시료군이 20% 시료와 함께 특성이 강하게 평가되었다. 쿠키의 기호도 검사 결과 쿠키의 고소한 맛에서 20% 시료군이 10% 시료군과 함께 유의적으로 높은 기호도를 나타내었고 전반적인 기호도에서는 20% 시료군이 기호도가 높은 경향을 나타내었다. 난소화성 전분을 10%와 20%로 대체한 시료군은 대조군과 비교하였을 때 유의적인 차이를 보이지 않으며 기호도 특성들이 높게 평가되어 기능성을 가진 슈거쿠키의 개발 가능성을 보여주었으며, 특히 10% 시료군은 경제적인 측면에서 바람직한 대체비율이라 생각된다.

This study was examined closely physiological activation and intended to present the possibility of developing food low-fat food. Developing carbohydrate fat replacer as materials for low-fat food made of Korean potato starch, it is expected that the new demand of fat replacer will be created. Potato starch was modified by chemical modification. The calorie of starch of GPS was measured to be 3.0 kcal/g, those of chemically modified starch, HPR showed 2.5 kcal/g respectively, suggesting that calorie is decreased by modified treatment. The appropriateness of processing food was experimented by substituting the existing oil and fat containing food with gel of starch and modified starch in constant rate through utilization of modified starch. When producing mayonnaise by substituting edible oil with gel of modified starch in 10-50%, calorie was reduced by 44~45% when substituted by 50%, suggesting the potential of low-fat food. Measuring viscosity of mayonnaise by Brookfield viscometer, the mayonnaise with HPR showed high viscosity and the chemical modified starch group of EZ also showed high viscosity. Generally, the material property of mayonnaise tended to reduce in all measured items when oil and fat are substituted by starch substituting materials and the substituting materials increase. When it comes to the emulsification stability of mayonnaise with starch substituting materials, emulsification stability of all mayonnaise with starch substituting materials is lower than that of compared group. While the group with NL as commercial fat replacer showed emulsification stability which was slightly higher than group with modified starch and the substitution group of HPR showed higher emulsification stability. Sensory evaluation for low-fat mayonnaise by substituting oil the products substituted by modified starch was more preferred than general starch substituting products such as GPS. While NL as commercially fat replacer showed the hight preference, products with H40, EZ were also highly preferred.

Developing carbohydrate fat replacer as materials for low-calorie and low-fat food made of Korean potato, it is expected that the new demand of fat replacer will be created. Potato starch was modified by chemical modification. Observing modified starch(treated in different method) by SEM, EZ(treated by enzyme) showed shape of deformed round oval, AC(treated by acetylation,), HPR(treated by hydropropylation) showed shape similar to that of NL(N-Lite), the commercial fat replacer. In the modified starch such as AC, peak in B and C type similar to those of general starch was found, but EZ showed non-crystalline shape. Compared to other modified starch, HPR, the chemically produced denatured starch showed very peculiar peak and structure in V-form. While the order of contents of amylopectin was in the order HPR 〉 EZ 〉 AC showed extremely high contents. Measuring the degree of gelatinization per the modified method, the degree of gelatinization of HPR as much higher than others. The water binding activity of modified starch was 240% in HPR. Measuring viscosity by producing general starch and modified starch as gel of 10% concentration, the CPS showed very high viscosity of 30.30 × 103 cp. Showing viscosity of 38.60, 31.60 103 × cp, the modified starch was in the order of HPR. While the calorie of starch of GPS was measured to be 3.0 Kcal/g, very low calorie those of chemically modified starch, HPR showed 2.5 Kcal/g respectively, suggesting that calorie is decreased by modified treatment. The appropriateness of processing food was experimented by substituting the existing oil and fat containing food with saturated gel of starch and modified starch in constant rate through utilization of modified starch. Therefore, research and development for materials and related products which maintain the existing quality and reduce fat contents will be constantly performed in the future.

본 연구에서는 난소화성 전분의 대체비율을 0%, 10%, 20% 및 30%로 하여 호두 빵을 제조하고 이화학적 검사, 관능검사 및 기호도 검사를 통하여 품질 특성을 조사하였다. 반죽의 pH는 10% 시료군과 대조군이 높은 값을 나타내었다. 반죽의 수분함량은 난소화성 전분을 대체한 시료군이 대조군에 비해 유의적으로 높은 값을 나타내었다. 완성된 빵의 부피는 대조군이 가장 큰 것으로 평가되었으나 10%와 20% 시료군과는 유의적인 차이를 보이지 않았다. 빵의 밝은 정도는 난소화성 전분을 대체한 비율이 높을수록 유의적으로 낮게 평가되었다. 빵의 경도는 10%와 20% 시료군이 대조군보다 유의적으로 낮은 특성을 나타내었다. 빵의 검성과 깨짐성은 비슷한 경향을 보이며 대조군과 30% 시료군이 강하게 평가되었다. 빵의 관능검사를 실시한 결과 고소한 맛은 대조군이 가장 특성이 강하게 평가되었고 떫은맛은 난소화성 전분의 대체비율이 높아짐에 따라 유의적으로 증가하는 것으로 나타내었다. 난소화성 전분의 대체비율이 높아질수록 떫은맛이 강해지므로 향후 떫은맛을 개선시키는 연구가 필요하다고 사료된다. 빵의 질긴 정도는 10%와 20% 시료군이 대조군보다 유의적으로 낮은 특성을 나타내어 부드럽게 평가되었다. 빵 내부의 조밀성은 대조군이 높게 평가되었으나 30% 시료군과는 유의적인 차이를 보이지 않았다. 빵의 기호도 검사 결과 조직감의 기호도는 10% 시료군이 대조군보다 유의적으로 높은 기호도를 나타내었다. 난소화성 전분으로 10%와 20%로 대체된 빵은 대조구와 비교하였을 때 유의적인 차이가 없거나 유의적으로 높은 기호도를 나타내어 개발 가능성을 보여주었으며, 아울러 품질 향상을 위한 체계적인 후속 연구가 계속 이루어져야 할 것으로 사료된다.

Tarakjuk with different amylose content was made up using roasted rice flours that consisted of the highest enzyme-resistant starch (RS), while differential scanning calorimetry (DSC) was also utilized to measure the gelatinization temperature of these roasted rice flours in order to establish cooking temperature of tarakjuk. The following qualities of tarakjuk with different amylose content were studied: color, viscosity, spreadability, starch fractions involving total starch (TS), rapidly digestible starch (RDS), slowly digestible starch (SDS) and RS, in vitro starch digestibility (IVSD) and sensory properties. During experimentation, it was found that as the amylose content of the rice flour decreased, the L value of tarakjuk decreased, whereas a value increased significantly (p〈0.05). Also, while viscosity showed to increase significantly (p〈0.05), on the opposite end, the property of spreadability decreased. TS ranged from 15.95~17.31%, RDS 9.36~10.16%, SDS 5.46~6.91% and RS 0.33~1.07%, on a dry basis. Although the amylose content of rice flours decreased, IVSD increased, however showing no significant difference. When testing the sensory properties of tarakjuk, color and viscosity increased, whereas clumpiness decreased. Ilpum tarakjuk showed the highest score for nutty taste and overall acceptance levels. In fact a high correlation was shown between nutty taste and overall acceptance level (p〈0.01), which leads one to believe that nutty taste is a prime factor that greatly influences overall acceptance. Furthermore, viscosity was positively correlated with both a and b values, however negatively correlated with L value (p〈0.05). Moreover, roasted nutty taste and overall acceptance were positively correlated with a value (p〈0.05), respectively. In conclusion, the above results suggest that tarakjuk could be made by choosing the appropriate rice flour based on the nutritional or sensory purpose.