This study selected the optimal conditions for enzyme treatment that can promote the extraction of functional components from Salvia plebeia (SP) and confirmed solubilization characteristics, functional component contents, and applicability evaluation in pilot-scale, antioxidative, and anti-inflammatory activity. The optimal conditions of pectinase, 1%, and 2 h (P) were determined through the enzyme treatment of SP. The water-soluble index and total polyphenol content were the highest in P compared to 100oC reflux condition for 2 h (CON). Component analysis performed using liquid chromatography-mass spectrometry revealed that flavonoid contents were increased 6.6-9.5 times in the P compared to CON. There was no difference in component content when comparing the measurement lab-scale and pilot scale. CON and P indicated cytoprotective effect against H2O2-induced RAW 264.7 cells. CON and P pre-treatment also reduced the production of nitric oxide and proinflammatory cytokines in LPS-induced RAW 264.7 cells. Furthermore, P showed higher antioxidant and anti-inflammation activities than CON. These results suggested that P has a higher concentration of bioactive compounds through enzyme treatment than that obtained from CON. Thus, it can be used as a primary material for industrial utilization, such as developing functional food materials using SP.
This study was performed to investigate the quality characteristics and antioxidant activity of black soybean Sunsik product with functional food ingredients and functional labeling system. We prepared black soybean Sunsik (BS) containing black beans, cereals, and vegetables. Black soybean Sunsik with nondigestible maltodextrin and calcium lactate (BSN) was prepared by adding non-digestible maltodextrin and calcium lactate to the base recipe to apply a functional labeling system. The particle size in BS was 118.00 μm, whereas BSN was 127.00 μm. The respective L, a, and b color values of BS were 73.25, 2.36, and 14.21. The respective L, a, and b values of BSN were 73.21, 2.36, and 14.31. The respective water retention capacities of BS and BSN were 241.67% and 216.33%. No significant difference was observed between BS and BSN in the three physicochemical properties described above. However, the pH of BSN was 5.45, which was significantly lower than that of BS. The total respective phenolic contents of BS and BSN were 1.75 mg GAE/g and 1.61 mg GAE/g, and total respective flavonoid contents of BS and BSN were 6.36 mg RE/g and 5.95 mg RE/g. The antioxidant capacities of BS and BSN were compared via assays of DPPH and ABTS radical scavenging activities, FRAP, and reducing power. The antioxidant activities of BS and BSN increased in a dose-dependant manner. No significant difference between BS and BSN was observed in any measure of antioxidant capacity. These results suggested that the addition of functional food ingredients (non-digestible maltodextrin and calcium lactate) did not affect the quality characteristics and antioxidant activity of black soybean Sunsik.
The objectives of this study are to increase the contents of functional compounds and antioxidant activity obtained from ginger (Zingiber officinale Roscoe) extracts using fermentation and enzyme. The optimal conditions (1%, Aspergillus luchuensis) for extraction methods were determined through fermentation treatment of ginger (concentrations of 0.5 and 1%; Aspergillus oryzae and Aspergillus luchuensis). The quality characteristics of the ginger extract for each treatment method (HW, hot water treated; EZ, enzyme; AL, fermentation; ALEZ, fermentation and enzyme) were observed using the optimal conditions. The water-soluble index (WSI) and total sugar content of the ALEZ increased by 2.6 times and 6.5 times, respectively, compared with that of the HW. However, the sum of gingerol and shogaol contents of ALEZ were 73.9% in ratio compared with lower than that of the HW. Antioxidant contents were generally higher in ALEZ, similar to the WSI trend. Therefore, ALEZ was more effective in enhancing antioxidant activity than EZ and AL. The fermentation and enzymatic approach described in this study would be beneficial to food industries for developing ginger functional products and materials.
This study aimed to evaluate the changes in the content of functional components and antioxidant activity of rice and barley according to the milling process used. A considerable amount of γ-oryzanol was observed in unmilled rice and barley. However, γ-oryzanol was not detected in the rice and barley after the milling process. The highest content of γ-aminobutyric acid (GABA) was observed in the unmilled Keunalbori-1-ho barley. The contents of biotin in all samples also decreased in the milled grains compared to the unmilled grains. The highest content of total polyphenols and flavonoids was observed in the Heuksujeongchal barley, and the highest radical scavenging activity was also found in this grain. The milling process led to a decrease in the content of functional components, including γ-oryzanol, GABA, biotin, polyphenols, and flavonoids in both rice and barley. These results may be useful in the development of processed foods using cereal grains.
Background: Stroke patients have weak trunk muscle strength due to brain injury, so a single type of exercise is advised for restoring functionality. However, even after intervention, the problem still lies and it is suggested that another intervention method should be applied with exercise in order to deal with such problem.
Objectives: To Investigate the effect of bridge exercise combined with functional electrical stimulation (FES) on trunk muscle activity and balance in stroke patients.
Design: Randomized controlled trial.
Methods: From July to August 2020, twenty stroke patients was sampled, ten patients who mediated bridge exercises combined with functional electrical stimulation were assigned to experiment group I, and ten patients who mediated general bridge exercises were assigned to experiment groupⅡ. For the pre-test, using surface EMG were measured paralyzed rectus abdominis, erector spinae, transverse abdominis/internal oblique muscle activity, and using trunk impairment scale were measured balance. In order to find out immediate effect after intervention, post-test was measured immediately same way pre-test.
Results: Change in balance didn’t show significant difference within and between groups, but muscle activity of trunk was significant difference rectus abdominis and erector spinae within groups I (P<.01), also between groups was significant difference (P<.05).
Conclusion: Bridge exercise combined with FES could improve trunk function more effectively than general bridge exercise due to physiological effect of functional electrical stimulation.
The purpose of this study was to investigate the changes in the content of functional components and the antioxidant activity of cooked rice and porridge composed of selected grains. The results of the study showed that brown rice and oat contained considerable amounts of γ-oryzanol (78.099~238.566 mg/100 g). However, cooked rice showed a decreased content of the same. The highest content of γ-aminobutyric acid (GABA) was observed in brown rice from Samgwang. The contents of biotin in all samples also decreased in cooked rice compared to raw cereal grains. The highest content of total polyphenols and flavonoids were observed in Heukjinmi rice, and the highest radical scavenging activity was also found in this variety. The cooking process led to a decrease in the content of functional components including γ-oryzanol, GABA, biotin, polyphenols, and flavonoids versus the corresponding raw cereal grains. These results may be useful in the development and application of home meal replacements using cereal grains.
The purpose of this study was to prepare Korean traditional vinegar using ginseng radices (wild simulated ginseng, red ginseng), and to investigate its functional ingredients and functionality. The total amino acid content of red ginseng vinegar and wild simulated ginseng vinegar was 124.36 mg% and 168.37 mg%, respectively, which was confirmed to be higher than the total amino acid content of red ginseng and wild simulated ginseng (70.71 mg%, 76.29 mg%). In addition, the polyphenol contents of red ginseng vinegar and wild simulated ginseng vinegar were 73.26 mg/g and 82.43 mg/g, respectively, which was confirmed to be higher than that of red ginseng and wild simulated ginseng (43.42 mg/g, 51.32 mg/g). And also, the DPPH electron donating ability of red ginseng vinegar and wild simulated ginseng vinegar was 893.26 μg/mL, 803.92 μg/mL, which showed that the scavenging ability was better than that of red ginseng and wild simulated ginseng (1253.21 μg/mL, 1021.01 μg/mL). On the other hand, it was confirmed that red ginseng vinegar and wild simulated ginseng vinegar contain 0.024% and 0.028% of compound K, a unique component of fermented ginseng, which is not contained in red ginseng and wild simulated ginseng. From these results, it was confirmed that Korean traditional vinegar using ginseng radices is a traditional functional food with functional ingredients and antioxidant activity.
Sorghum is a rich source of various phytochemicals including phenolic compounds that have potential to significantly benefit human health. Phytochemical production may be induced not only by genotype but also by a number of environmental factors including temperature and an amount of sunshine. The objective of this study was to determine the effect of planting dates and harvesting stages on the quality traits of ‘Donganme,’ a grain sorghum variety developed to produce high antioxidants. ‘Donganme’ was planted in three locations on four dates from early May to early July. Each planted fraction was harvested 35, 40, 45, 50, and 55 days after the head shooting date, respectively. The results showed that significant differences existed between the growth period and content of polyphenol and flavonoid. The sorghum harvested at 35 days after head shooting had the higher polyphenol and flavonoid content in all planting times and the tannin content was increased as the planting date was delayed. So the sorghum grown in late planting dates (July 5) had highest polyphenol content (2.07 gGAE/100g) at early harvesting (35 days) while late planting gave the low grain yield (1.3 ton/ha). Polyphenol index to average temperature during 35 and 45 days after heading of ‘Donganme’ showed that total polyphenol contents were decreased by 5.2% and 4.4%, respectively, with increasing average temperature of 1oC. And high correlation (R=0.85) was observed between accumulated temperature and the amount of polyphenol of ‘Donganme’, confirming the major contribution of the quality traits is temperature. To produce high quality grain from sorghum the relation between the yield and nutrition components must be considered simultaneously (i.e., Sorghum produced the highest polyphenol and flavonoid when planted in June 15 and harvested at 40 to 45 days after head shooting .
To compare functional Chinese cabbage(‘Amtak’ baechu; F1 hybrid cultivar between Brassica rapa and B. perkinensis, AB) with general Chinese cabbage (‘Chunkwang’ baechu; general spring cultivar, CB), two kinds of kimchi(ABK and CBK) prepared with AB and CB cultivar were fermented at 10°C for 10 days. Their fermentative characteristics and anti-proliferative activities against mouse carcinoma cell lines were investigated. General kimchi(CBK) showed mature pH on the 6th day of fermentation, whereas functional kimchi(ABK) reached pH on the 9th day. CBK also exhibited acidity of mature stage on the 6th day, but ABK reached mature acidity on the 9th day. Although ABK and CBK were salted in the same condition, ABK had lower salinity than CBK, throughout the fermentation time. The highest total bacterial and lactic bacterial counts of CBK showed on the 8th day of fermentation, but ABK showed the highest total bacterial and lactic bacterial counts on the 10th day. The texture of ABK was harder than CBK for fermentation time. This seems to be corrleated with the slower fermentation rate of ABK. ABK showed significantly higher anti-proliferative activity (54.6% cell viability of control) in B16BL6 at 1,000 μg/mL. ABK was also higher in anti-proliferative activity than CBK throughout the fermentation time. However, there was no significant difference in the anti-proliferative activity of ABK between the fermentation times. In conclusion, fermentation of ABK showed a better texture, due to the slow fermentation rate and more anti-proliferative activity against mouse carcinoma cell line than those of CBK.
This study investigated the changes in chemical components, antioxidant compounds, and activity before (BG) and after germinated (AG) brown rice in order to promote the availability of these beneficial factors. The GABA content of BG and AG brown rice were 0.07~6.61 and 11.13~49.72 mg/100 g, respectively. The α-amylase activity of AG brown rice was 1.77~70.25 unit/g fold higher than it was in BG brown rice. Total polyphenol and flavonoid contents, which are known to be related to antioxidation, were higher in BG brown rice than in AG brown rice. The total polyphenol contents of BG and AG brown rice were 10.52~36.38 and 11.38~26.33 mg/100 g, and the total flavonoid contents were 3.55~13.39 and 3.52~9.78 mg/100 g, respectively. Also, DPPH radical scavenging activity was 57.64~251.34 and 50.49~213.35 mg TE/100 g, respectively. ABTS radical scavenging activity and total tannin content showed a similar trend to DPPH radical scavenging activity. We expect that this data will be useful in the manufacturing of food products.
This study was carried out to investigate the antioxidant activity and changes in major functional components of fermented Gastrodia elata Blume. Fermented G. elata Blume powder by Phellinus linteus repeated thrice (3rd FGP) showed more DPPH radical scavenging activity than a non-fermented G. elata Blume powder (NFGP), and once fermented G. elata Blume powder (1st FGP) at a concentration of 500 and 1,000 ppm. Free radical scavenging activity of 3rd FGP was similar to that of BHA at a dose of 1,000 ppm. Moreover, the ABTS radical scavenging activity of the 3rd FGP increased compared to NFGP and 1st FGP at a concentration of 31.25 ppm. Total polyphenols and flavonoid contents were increased as fermentation progressed. Ergothioneine content was increased more than 8 times in the 1st FGP, 3 times in the 3rd FGP, respectively than NFGP. In conclusion, this study indicated that the antioxidant activity and functional component contents of G. elata Blume were increased depending on the fermentation number.
This study compared the antioxidant activity and functional constituents of the peel, flesh,and placenta of oriental melons according to the harvest time. The sample oriental melons were harvested during the major harvest time (from June to August) and divided into peel, flesh, and placenta. To examine the antioxidant activity and functional constituents, the sample melons were extracted using EtOH. As a result, the functional constituents and antioxidant activity of the oriental melons were found to change according to the harvest time. The total phenol and total flavonoid contents were highest in the samples harvested in June, and lowest in the samples harvested in July. Meanwhile, the antioxidant activities were estimated using an ABTS and FRAP assay, where the samples harvested in June also showed the strongest the antioxidant potential, while the samples harvested in July showed significantly lower antioxidant activities. This means that the climate influences the synthesis of secondary metabolites. The above data also suggests that oriental melons harvested in June contain more functional phytochemicals, making them more beneficial for human health.
목적 : 본 연구의 목적은 시각되먹임 제공 및 차단으로 시행된 자세수직훈련의 효과를 비교함으로써, 시각되먹임을 차단한 자세수직훈련이 뇌졸중 밀기증후군 환자의 기능회복 및 일상생활동작에 어떠한 영향을 미치는지 알아보고 자 하였다. 연구방법 : 본 연구는 개별사례 교차중재 연구설계 중 대상자간 다중기초선을 이용하였으며, 밀기증후군 환자 3명 을 대상으로 하였다. 기초선 측정은 대상자 1이 4회, 대상자 2가 7회 그리고 대상자 3이 10회였으며, 치료회기는 대상자 1이 14회, 대상자 2가 11회 그리고 대상자 3이 8회였다. 치료중재는 대상자 1이 14회, 대상자 2가 11회 그 리고 대상자 3이 8회였다. 밀기증상의 기능회복 평가는 밀기 증후 척도, 일상생활동작을 평가하기 위하여 바델지 수 및 실험 전∙후 대칭적인 서기자세를 평가하기 위하여 근전도기로 양측 중둔근에서 근활성도를 측정하였다. 결과 : 시각되먹임을 차단한 자세수직훈련이 적용되었을 때 대상자 1, 2, 3의 밀기 증후 척도는 각각 기초선과 비교 하여 52.4%(5.3점에서 2.8점), 36.4%(4.4점에서 1.6점) 및 23.6%(3.6점에서 0.9점) 향상되었다. 시각되먹임을 제공한 자세수직훈련은 42.9%(5.3점에서 2.3점), 26.1%(4.4점에서 1.2점) 및 13.2%(3.6점에서 0.5점) 향상되었다. 대상자 1, 2, 3의 바델지수는 각각 기초선과 비교하여 22.4%(44.3점에서 54.1점), 15.5%(44.7점에서 51.6 점) 및 15.8%(51.5점에서 59.6점) 향상되었다. 시각되먹임을 제공한 자세수직훈련은 17.0%(44.3점에서 51.8점), 11.4%(44.7점에서 49.8점) 및 14.8%(51.5점에서 59.1점) 향상되었다. 또한 실험 전과 비교하여 실험 후 환측의 중둔근 근활성도가 더 크게 향상되었다. 결론 : 교대로 적용된 치료중재 결과를 통하여 시각되먹임을 차단한 자세수직훈련이 시각되먹임을 제공한 자세수직훈련보다 밀기증후군 환자의 기능회복 및 일상생활동작능력에 더 효과적인 것으로 나타났다. 향후에는 많은 대상자를 포함시키고 오랜 기간 동안 적용한 연구들이 계속 이어져야 할 것이다.
한우 고급육 생산을 위한 사양관리에 있어 기능성 사료의 활용 가능성을 규명하기 위해 뽕나무 사일리지의 기능성 성분 및 항산화 활성효능을 분석하였다. 뽕나무 사일리지의 일반 성분을 분석한 결과, 건물함량 , 조단백질 함량 , 조지방 함량 , 조섬유 함량 및 조회분 함량 였다. 뽕나무 사일리지에 함유된 활성성분의 분석 결과, 대표적 활성성분으로 혈당강하성분인 1-deoxynojirimycin(1-DNJ)의 함량이 0.568mg/g으로 검량되었으며 혈압강하성
느타리버섯의 배지에 철, 고춧가루, 은행잎을 첨가하였을 때 버섯의 생육과 생리활성을 분석한 결과는 다음과 같다. 수량은 춘추 품종에 철 34.9g을 첨가하였을 때 144g으로 가장 높았고, 나머지 품종간에는 차이가 없었으며, 34.9g 및 174.5g 처리시 17.4g 처리에 비해 수량이 높은 경향이었다. 자실체와 배지에 포함된 무기물의 함량은 종류별로 차이가 있었으며, 배지중 철의 함량이 증가하여도 자실체내의 철의 함량은 0.31∼0.43mg/kg으로 거의 일정하게 유지되었다. 배지에 포함된 고춧가루의 함량이 많아질수록 자실체에 포함된 캡사이신의 함량도 많아졌으며, 수한품종에서 6mg/100g으로 가장 많이 전이되었다. 철이나 고추를 첨가한 배지에서 재배한 버섯에서 페놀성 화합물의 함량이 높게 나타났으며, 항산화력과 총 페놀성 화합물 함량, 환원력과 총페놀성 화합물 함량, 금속이온 제거능과 페놀성 화합물의 함량 간에는 서로 영향을 미치지 않는 경향이었다. Macrophage 활성화능은 수한 품종이 다른 품종에 비해 높은 경향이었다.
본 연구의 목적은 기능적 근적외선 분광법인 fNIRS(functional Near-Infrared Spectroscopy)를 활용하여 미술표현에 따른 뇌 활성화를 측정하는 것이다. fNIRS는 소형의 크기에, 휴대성이 용이하고, 근적외선이 연구참여자의 전두엽에 비침습적으로 침투하여 대뇌의 혈류량을 파악하고, 이를 토대로 뇌의 활성화 상태를 측정하는 장비이다. K대학 소재의 연구참여자 4명을 대상으로 fNIRS 를 착용 후 1분 30초씩, 총 3번에 걸쳐서 미술표현 활동을 진행하였고, 개별 뇌 활성 이미지와 4명 의 평균 뇌 활성 이미지 및 그래프를 추출하였다. 분석결과 연구참여자 4명의 모든 미술표현 활동에서 시각적 작업기억을 담당하는 우측의 DLPFC영역, 메타인지와 평가 및 성찰을 담당하는 FPC 영역, 그리고 가치 부여 및 판단을 담당하는 OFC영역이 활성화 되었음을 확인하였다. 분석결과를 토대로 미술교육의 시사점 및 나아가야 할 방향을 제안하였다. 첫째, 미술활동은 우뇌와 좌뇌의 활성이 함께 일어나지만 비교적 우뇌가 더 많이 활성화 된다. 둘째, 미술표현활동은 주의집중과 목표지향적 행동, 동기부여를 유발하는 교과이다. 셋째, 미술표현 활동에서 자기평가와 성찰을 위해 표현 후 결과물에 대해 발표 및 소감문을 작성해 보는 시간이 필요하다. 넷째, 미술할동에서 주제를 제시할 때 막연하고 추상적인 주제가 아니라 구체적인 언어와 단어로써 제시해야 한다. 본 연구를 토대로 앞으로 미술교과와 관련된 과학적이고 객관적, 실증적인 연구가 많이 진행되어 미술교과의 위상을 높이고, 더 좋은 후속연구들이 이루어지기를 기대한다.
The objective of this study was to identify the anti-oxidation, astringent, and inhibition effects of wild Ligularia fischeri on hyaluronidase and angiotensin conerting enzyme (ACE). In order to identify the total phenolic compound (TPC), various solvents were used for extraction showing hot water extract with the highest value of 14.42 GAE mg/g. In addition, ABTS radical scavenging activity measurements revealed an anti-oxdiation effect of 98.64-99.84% a hot water extract concentration of 50-200 μg/mL and a radical scavenging activity of 95.14-98.96% at a 60% ethanol extract content. If expressed in antioxidant protection factors (PF), the hot water extract showed 0.59-1.02 PF and the 60% ethanol sample displayed 0.30-0.74 PF. To identify the bio-activity effect, the hyaluronidase inhibition effect was determined as 4.66-35.00% in a 50-200 μg/mL hot water extract. Considering ACE inhibition effect, the hot water extract and 60% ethanol sample showed 0-64.24% and 46.12-69.64% inhibition effect, respectively. Lastly, when taking into account the astringent effect, the hot water extract with 50-200 μg/mL TPC concentration showed 15.68-26.92% and the 60% ethanol sample with an equal concentration exhibited 49.48-86.84%, which indicates the possibility to apply this product as a cosmetic source for pore contraction. Therefore, wild Ligularia fischeri extract can be used for anti-inflammation, high-blood pressure prevention, and as a source for health functional food with anti-oxidative properties.
Little attention has been paid to the functional aspect of the flower petal of Paeonia lactiflora, compared to that of its root. To determine the components of flower petal of Paeonia lactiflora, we conducted the Fourier transform ion cyclotron resonance (FT-ICR) MASS spectrophotometric analysis. We detected the 24 different types of ingredients from the 70% ethanol extracts of flower petal of peonia lactiflora cv. ‘Red Charm’. The main compounds were quercetin glucopyranosides, methyl gallate, paonioflolol and kaemperol glucopyranosides. We further tested its functional activity. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of the extracts was 87.9-90.4% at 0.1mg/ml. This result showed that these flower extracts have approximately 5-fold stronger antioxidant potential than a previous report with root extracts (Bang et al. 1999). The result of tyrosinase inhibition assay of Paeonia lactflora extract was almost similar to that of arbutin except significantly higher effect in the coral sunset extract at 0.1% concentration. Hyaluronidase inhibition assay showed 76.5% inhibition at 5% concentration of this flower extract, indicating that Peaonia lactiflora flower extracts have the major anti-inflammatory, anti-oxidant and brightening effects. Taken together, these results suggest these three Paeonia lactiflora species extracts might provide the basis to develop a new natural brightening agent.
Sensory evaluation, in vitro antioxidant activities and main compounds of coffee water-extract, coffee liqueur (CL) and coffee-ground liqueur (CGL) were investigated to consider their industrialization. Sensory evaluation showed that all groups of CGL without 25% CGL (3 month) were relatively higher than CL groups. Total phenolic compounds and in vitro antioxidant activities such as 1,1-diphenyl-2picryl-hydrazyl (DPPH) radical scavenging activity and ferric reducing/antioxidant power (FRAP) were also performed. The group of 35% CGL had higher total phenolic compounds than others, and the result of DPPH radical scavenging activity was similar to that of total phenolic compounds. In addition, 35% CGL is comparable to the FRAP of coffee water extract (CE). Qualitative and quantitative analysis using high-performance liquid chromatography (HPLC) were performed, and chlorogenic acid as a ployphenolic compound and caffeine as a nonpolyphenolic compound were detected in all samples. Moreover, the HPLC analysis showed that CGLs contain a larger amounts of chlorogenic acid (difference of 0.3~10.5%) and also greater amounts of caffeine (difference of 10.0~18.2%) more then CE. Consequently, these results suggest that coffee-ground as coffee by-products could be used as commercially available food substances because of its physiological molecules remained.
본 연구는 유색미 품종들의 적정 재배시기와 지역에 따른 색소발현 및 항산화 활성 변이를 분석하여 고품질의 유색미를 생산할 수 있는 적정 재배관리기술의 기초자료를 얻고자 수행하였고 그 결과는 다음과 같다.
1. 밀양, 봉화, 상주, 의성 4지역에서 이앙시기를 5월 20일, 6월 5일, 6월 20일 3시기에 걸쳐서 흑미 3품종과 적미 2품종을 재배한 결과 출수 후 30일간의 평균기온 19.0℃ 이상에서 평균기온이 1℃ 증가할 때마다 흑미의 안토시아닌 함량은 약 9.9%, 적미의 총폴리페놀 함량은 약 9%씩 감소하였다.
2. 유색미의 기능성물질 함량을 증대시키기 위한 출수 후 30일간 적정등숙온도는 흑미의 경우 조생종은 22~23℃, 중만생종은 21~22℃의 범위였으며, 적미도 21~22℃ 범위였다.
3. 전국 지역별 30년간의 기상자료를 토대로 적정출수기를 추정한 결과 제천지역은 조생종이 8월 11일에서 8월 17일, 중만생종은 8월 25일경이며 보은지역은 조생종이 8월 15일에서 8월 19일, 중만생종은 8월 20일에서 8월 27일에 해당되었다. 영주지역은 조생종이 8월 15일에서 8월 21일, 중만생종은 8월 24일 경이었고, 충주지역은 조생종이 8월 17일에서 8월 23일, 중만생종은 8월 22일에서 8월 29일에 해당되었다. 대구지역은 조생종이 8월 27일에서 9월 2일, 중만생종은 9월 3일에서 9월 6일경으로 주요 재배적지는 제천, 보은, 문경, 영주 등이었다.
4. 적정파종기 추정결과 제천지역은 조생종이 5월 18일에서 5월 26일, 중만생종이 4월 11일 경이었으며, 보은지역은 조생종이 5월 23일에서 5월 28일, 중만생종이 4월 9일에서 4월 26일, 문경지역은 조생종이 5월 24일에서 6월 1일, 중만생종이 4월 21일에서 5월 4일, 영주지역은 조생종이 5월 22일에서 5월 30일, 중만생종이 4월 13일에서 4월 27일, 충주지역은 조생종이 5월 31일에서 6월 7일, 중만생종은 5월 4일에서 5월 14일 경으로 추정되었다.