The purpose of this study was to investigate nutritional and functional ingredients and antioxidant activity after fermenting rice bran using Lactobacillus brevis for 24, 36, and 48 hours. The results of the analysis of the nutritional ingredients revealed that there was no significant difference in the carbohydrate, crude protein, crude fat and ash content regardless of the fermentation process and fermentation time. The amount of dietary fiber was significantly different between the unfermented and fermented rice bran and was observed to be the highest after a 48-hour fermentation. The -oryzanol, gamma-aminobutyric acid (GABA) and total phenolic contents were significantly higher in the fermented rice bran compared to the unfermented rice bran (p<0.05) and the GABA and total phenolic contents increased significantly as the fermentation time increased (p<0.05). The 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis-3-ethylbenzothiazoline-6- sulfonic acid (ABTS), superoxide and hydroxyl radical scavenging activities used to measure antioxidant effects significantly increased as the fermentation time increased (p<0.05). From these results, it was confirmed that the antioxidant effect and functional components, namely -oryzanol, GABA, and the total phenolic content of rice bran improved with fermentation. Based on these results, fermented rice bran could be presented as a functional material for use in high value-added industries.

본 연구는 새만금 간척지와 주산지인 무안에서 재배하였을 때 양파의 생육과 퀘르세틴 등 기능성 성분을 분석하였다. 양파는 중생종 품종인 ‘맵시황’, 중만생 품종인 ‘선파워’, ‘헬시큐’를 사용하였다. 전남 무안에 9월 상순 파종 후 육묘하여 간척지와 무안에 각각 11월 상순에 정식하고 이듬해 6월 초순에 수확하였다. 중만생종 품종인 ‘선파워’와 ‘헬시큐’가 간척지에서 구중 336.5g, 248.3g으로 생육이 우수하였다. 피루브산은 ‘헬시큐’, ‘맵시황’, ‘선파워’ 순으로 높았고 지역 별로는 ‘헬시큐’, ‘맵시황’ 품종이 간척지 재배 시 각각 48.3μmol·g-1, 41.1μmol·g-1으로 무안에서 재배하였을 때보다 높았다. 총 퀘르세틴은 품종 별로 비교하였을 때 ‘헬시큐’가 219.1μg·g-1으로 가장 높았고 ‘선파워’, ‘맵시황’의 순이었으며 재배지역에 따른 차이는 확인되지 않았다. 또한 총 페놀은 총 퀘르세틴 함량과 유사한 경향을 보였으며, ‘헬시큐’의 함량이 853.7μg·mL-1으로 가장 높았고, ‘선파워’는 무안에서 재배할 경우 페놀 함량이 높은 것으로 나타났다. 결과적으로, 피루브산, 총 퀘르세틴, 총 페놀의 함량은 재배지역 보다는 품종에 따른 영향이 큰 것으로 판단되었다.

Flammulina velutipes, known as winter mushroom in the family of Physalacriaceae, is the main edible and export mushroom with the third highest production after oyster and king oyster mushroom in Korea. However, as normal consumers regard F. velutipes as a simple subsidiary material, there is a limitation to increasing mushroom demand. In order to overcome the consumption limit and increase the differentiation of new varieties, it is necessary to breed varieties with enhanced functionality in consideration of consumer preferences. Therefore, the study was performed to analyze nutrient components and several useful functional substances with 26 genetic resources of F. velutipes. Analyses of inorganic compound(Ca, K, Mg) and 15 amino acids revealed that Strain 4148 had the highest content among the 26 strains. Beta-glucan, which increases immune activity and polyphenol, which exert antioxidant effects were higher in non-white strains than in white strains with a small number of exceptions. Among the five fatty acids, linoleic acid(an omega-6 fatty acid) and α-linolenic acid(an omega-3 fatty acid), were detected in six mushroom strains. α-linolenic acid, which was not found in five major mushrooms including oyster mushrooms, was identified in F. velutipes. The results of HPLC analysis showed that ‘Auram’ (Strain 4232) and ‘Baekseung’(Strain 4230) had the highest content of the stabilizing neurotransmitter GABA(15.38 μg/ml and 20.56 μg/ml, respectively) among non-white and white strains, respectively. Our findings provide useful information for breeding F. velutipes to obtain strains with enhanced functionality.

This study aimed to evaluate the changes in the content of functional components and antioxidant activity of rice and barley according to the milling process used. A considerable amount of γ-oryzanol was observed in unmilled rice and barley. However, γ-oryzanol was not detected in the rice and barley after the milling process. The highest content of γ-aminobutyric acid (GABA) was observed in the unmilled Keunalbori-1-ho barley. The contents of biotin in all samples also decreased in the milled grains compared to the unmilled grains. The highest content of total polyphenols and flavonoids was observed in the Heuksujeongchal barley, and the highest radical scavenging activity was also found in this grain. The milling process led to a decrease in the content of functional components, including γ-oryzanol, GABA, biotin, polyphenols, and flavonoids in both rice and barley. These results may be useful in the development of processed foods using cereal grains.

This study examines the effects of different cooking methods (stir-frying, steaming, superheated-steaming) on the contents and the true retention of moisture, water-soluble vitamins, and bioactive compounds of ten selected vegetables: broccoli, brussels sprout, cabbage, eggplant, green bean, onion, red cabbage, red onion, squash, and tomato. The total color difference (ΔE) values were decreased after stir-frying the samples, except for eggplant, green bean, and tomato. The true retention of water-soluble vitamins (B1, B2, B3, and C) was increased in most vegetables after superheated-steaming, as compared to steaming and stir-frying. Moreover, compared to the uncooked vegetables, a higher true retention of total polyphenol and flavonoid was obtained for most vegetables subsequent to superheated-steaming. Total anthocyanin content was detected only in eggplant, red cabbage, and red onion, and a smaller loss of anthocyanin was determined after subjecting red cabbage to superheated-steaming. Also, the free radical scavenging activities were higher in superheatedsteaming vegetables, except in eggplant and squash. These results indicate that superheated-steaming induces a positive effect for retaining water-soluble vitamins and functional components of vegetables.

The purpose of this study was to investigate the changes in the content of functional components and the antioxidant activity of cooked rice and porridge composed of selected grains. The results of the study showed that brown rice and oat contained considerable amounts of γ-oryzanol (78.099~238.566 mg/100 g). However, cooked rice showed a decreased content of the same. The highest content of γ-aminobutyric acid (GABA) was observed in brown rice from Samgwang. The contents of biotin in all samples also decreased in cooked rice compared to raw cereal grains. The highest content of total polyphenols and flavonoids were observed in Heukjinmi rice, and the highest radical scavenging activity was also found in this variety. The cooking process led to a decrease in the content of functional components including γ-oryzanol, GABA, biotin, polyphenols, and flavonoids versus the corresponding raw cereal grains. These results may be useful in the development and application of home meal replacements using cereal grains.

본 연구에서 흰목이버섯의 추출조건별 추출수율과 기능성 성분의 함량을 비교하여 최적 추출조건을 분석하였으며, 모든 분석에서 R²이 0.9331~0.9462로 유의성을 보이는 것을 확인하였다. 각 독립변수에 따른 추출수율과 ergothioneine, β-glucan 성분을 분석한 결과 추출수율은 추출온도와 추출시료농도에 반비례했으며, 추출시간에는 큰 영향이 없었다. Ergothioneine 성분은 온도가 증가할수록 증가하며, 추출시간은 4.33 h가 가장 높았고 시료량에 큰 영향이 없었다. β-glucan 성분은 온도가 증가할수록 감소했으며, 추출시간에 큰 영향이 없었고 시료량이 21.2 mg/mL에서 가장 높았다. 모든 종속변수의 최대 독립변수는 온도 60 o C, 추출시간 4.33 h, 추출시료농도 16.6 mg/mL에서 추출수율 24.9%, ergothioneine 성분함량 66.8 ug/g, β-glucan 성분함량 34.9 g/100 g으로 나타났다.

This study examined the contents of bioactive compounds and the biological activity of okra seed oil. Okra seed oil consisted mainly of linoleic acid (44.2%). The content of total phytosterols was 2.180 mg/g oil, with β-sitosterol being the highest (1.756 mg/g oil). The vitamin E content was 1.278 mg/g oil; the content of α-tocopherol was higher than γ- tocopherol. The total polyphenol and flavonoid contents were 2.463 mg gallic acid equivalent/g and 1.602 mg cathechin equivalent/g, respectively. The 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid and α-α-diphenyl-β-picrylhydrazyl free radical scavenging activities were 15.297% and 22.265%, respectively, and the reducing power was 4.524 mg gallic acid equivalent/g. The okra seed oil inhibited 77.692% of the α-glucosidase activity. The present study showed that okra seed oil had a considerable amount of phytochemicals and exhibited biological activity. These results suggest that okra seed oil is a potential natural therapeutic for the management of metabolic syndromes.

우엉을 9번 찌고 9번 말려서 차를 제조한 다음 M사의 우엉차와 성분 및 기능성을 비교 분석하였다. 개발한 우엉차는 칼로리 346.48kcal, 탄수화물 72.75g/100g, 단백질 11.98g/100g, 회분 5.01g/100g이었다. 무기물 함량은 칼륨 1,476.21 mg/100g, 칼슘 255.96 mg/100g, 마그네슘 311.41mg/100g 이었다. 유리당 총량은 31.93 mg/100g이었고 그중 fructose 26.27mg/100g , glucose 4.39 mg/100g, sucrose 1.27 mg/100g이었다. 포화지방산은 40.73 mg/100g, 불포화지방산은 59.28 ㎎ /100g이고 그 중 linoleic acid 41.28 mg/100g, palmitic acid 33.11 mg/100g, oleic acid 10.07 mg/100g , linolenic acid 7.92 ㎎/100g이었다. DPPH 라디컬 소거력은 개발한 차 33.9%, 비교용 M사 차 2.3%, 지표물질 37.2%였다. ABTS 라디컬 소거력은 개발한 차 90.7%, M사차 85.9%, 지표물질 47.6%였다. SOD 유사활성은 개발한 차 11.3%, 비교용차 50.5%였다. 플라보노이드 함량은 개발한 차 2.6 fold, M사차 2.9 fold, 지표물질 1.7 fold였다. 폴리페놀 함량은 개발한 차 33.8 fold, M사차 38.9 fold, 지표물질 13.4 fold였다. 기호도는 1회 우린 것과 5회 우린 것을 비교하였다. 1회 우린 것을 기준으로 할 때 5회 우린 것의 기호도 중 색은 개발한 차 65.9%, M사차 12.8%, 향기는 개발한 차 78.0%, M사차 33.3%, 맛은 개발한 차 71.4%, M사차 20.7%, 나타났다. 이같이 비교용 M사차는 우릴수록 추출 성분이 감소하여 5회 후에 종합적인 기호도는 21.4%로 감소한 반면 개발한 차는 감소폭이 적어서 72.1%를 나타냈다. 이같이 개발한 우엉차는 비교용 M사차 및 지표물질보다 항산화 작용이 강하고 유효 물질 함량도 더 많고, 기호성도 높으므로 질병 예방 및 개선 효과가 클 것으로 생각한다.

돼지감자를 9번 찌고 9번 말려서 차를 제조한 다음 M사의 돼지감자차와 성분 및 기능성을 비교 분석하였다. 개발한 돼지감자차는 칼로리 342.27kcal, 탄수화물 73.87g/100g, 유리당 32.66mg/100g, 회분 6.80g/100g, 단백질 8.21g/100g이었고 무기물 총량은 2,785.67mg/100g, 칼륨 2,563.93mg/g, 칼슘 97.52mg/g, 마그네슘 88.78mg/g 등이었다. 돼지감자차의 유리당 총량은 32.66mg/100g이고 그중 fructose 17.40mg/100g, sucrose 9.03mg/100g, glucose 6.05mg/100g이었다. 돼지감자차의 포화지방산은 30.34mg/100g, 4 불포화지방산은 69.66㎎/100g이었고 그 중 linoleic acid 47.00mg/100g, palmitic acid 25.31mg/100g, linolenic acid 8.61㎎/100g이었다. DPPH 라디컬 소거력은 개발한 차 34.2%, 비교용 M사차 5.2%, 지표물질 44.0%였다. ABTS 라디컬 소거력은 개발한 차 93.0%, M사차 61.9%, 지표물질 47.6%였다. SOD 유사활성은 개발한 차 2.7%, M사차 1.6%였다. 플라 보노이드 함량은 개발한 차 2.8 fold, M사차 2.0 fold, 지표물질 1.7 fold 였다. 폴리페놀 함량은 개발한 차 38.2 fold, M사차 8.92 fold, 지표물질 14.0 fold였다. α-Glucosidase 저해율은 개발한 차 9.83%, M사차 8.92%였다. 기호도는 1회 우린 것과 5회 우린 것을 비교하였다. 1회 우린 것을 기준으로 할 때 5회 우린 것의 기호도 중 색은 개발한 차 83.7%, 비교용 차 50.0%, 향기는 개발한 차 78.0%, 비교용 차 42.5%, 맛은 개발한 차 66.7%, 비교용 차 37.5%, 종합적인 기호도는 개발한 차 73.3%, 비교용 차 47.5%로 나타났다. 이같이 비교용 M사차는 우릴수록 추출 성분이 감소하여 5회 후에 종합적인 기호도는 46.3%로 감소한 반면 개발한 차는 감소폭이 적어서 73.3%를 나타냈다. 이같이 개발한 돼지감자차는 비교용 M사차 및 지표물질보다 항산화 작용이 강하고 유효 물질 함량도 더 많고, 기호성도 높으므로 질병 예방 및 개선 효과가 클 것으로 생각한다.

본 연구에서는 영지버섯의 종류별 기능성 성분과 생리활성과의 상관관계를 분석하였다. 영지버섯은 담자 균류 민주름목 구멍장이 버섯과에 속하며 전세계에 분보하고 떡갈나무, 상수리나무 등 활엽수에서 주로 자생한다. 영지버섯은 색상과 형상에 따라서 적, 흑,자, 황, 백, 청, 녹각, 편목, 쓰가 영지로 구분을 하고 있다. 영지버섯은 한방에서 골을 강화하고, 치질과 신경쇄약, 소화불량 등 만성질환에 도움을 주며, 오미의 성분 준다고 나와있다. 영지버섯은 2010~2013년 영지버섯의 전국 생산량은 281 M/T(metric ton), 경기도, 충남, 전북에서 많이 생산되었고, 주요 성분으로 triterpene계(ganoderic acid A-T), steroid계(ganoderol A,B), 다당류 (예: ganoderan B), proteoglycan(예: GLIS) 등이 알려져 있다. 영지버섯의 생리활성으로서는 항종양작용, 심혈관계에 대한 작용, 세포보호작용, 면역증진, 항바이러스 작용 등의 약리효능을 나타내는 것으로 보고되고 있고, 본연구팀의 이전 연구 결과에서 항산화 효과 및 RAW 264.7 대식세포에 대한 항염증 효과를 돟물 실험을 통해 분석한 결과 우수한 효과를 나타낸 것을 보고한 바 있다. 또한, 본 연구팀은 영지버섯중 녹각 영지버섯 추출물의 엡스타인 바 바이러스에 대해서 위암세포 발달을 저해하는 효과를 나타내는 것을 동물 실험을 통해서 확인하였다. 이번 연구결과는 영지버섯류의 기능성 성분과 생리활성 상관관계를 분석하여 베타 글루칸이외의 성분들과 효능과의 상관관계를 분석함으로서 버섯을 이용한 고부가가치 산업화의 소재 개발의 기초를 마련하고자 하였다.

Brown rice, barley, and buckwheat contain a variety of functional ingredients, such as vitamins, minerals, and dietary fibers. Germination is an effective technique for improving the digestion and absorption of the constituents in grains. In addition, germination could modify the chemical properties of grains and increase the content of biologically active substances. Many studies have reported that the intake of grains or germinated grains could ameliorate blood glucose level, stimulate the immune system, lower blood pressure, and result in anti-obesity effect. Therefore, grains and germinated grains are being utilized in a variety of functional products. The present study reviews the primary components and bio-functionalities of grains (brown rice, barley, and buckwheat) and germinated grains (germinated brown rice). The development of grain products for promoting health functional properties is also discussed.

Sesquiterpene lactones (SLs) are a group of over 500 compounds, characteristic of the Asteraceae. They are interesting from the chemical and chemotaxonomic point of view, and show antitumour, anti-leukaemic, anti-cardiovascular disease, reduction of inflammation, and anti-microbial activities. The SLs, free lactucin and lactucopicrin, content in 572 accessions of lettuce (Lactuca sp.) germplasm introduced from 30 countries were quantified using high-performance liquid chromatography. Variation range of free lactucin content exhibited trace ~ 235.3 μg from 1g of dried leaves and average was 41.2 ± 1.2 μg (Avr ± SE), variation range 55.0 ~ 3,041.0 μg and average 526.9 ± 17.5 μg in free lactucopicrin content, and variation range 66.3 ~ 3,188.5 μg and average 568.1 ± 18.1 μg in total free SLs content. Lactucopicrin occupied 92.7% of the total SLs content. Among the varietal types, crisphead type exhibited the highest average total free SLs content, next is leaf lettuce, and butterhead type lettuce exhibited the lowest that of content. German accessions exhibited the lowest average total free SLs content, Korean accessions exhibited the highest, and European origin accessions exhibited lower that of content. Red leaf color accessions having higher SLs content than that of green color. Seven accessions having more than 2,000 μg·g-1 dwt of total free SLs content and five accessions having less than 100 μg·g- 1dwt that of content. These accessions can be used as low SLs content cultivar breeding or high SLs content cultivar breeding sources as well as research materials for medical treatment such as, anti-tumour, anti-leukaemic, and antimicrobial activities, etc.

복분자를 개별인정형 건강기능식품 기능성 원료로 개발하기 위하여 지표성분 표준화를 위한 ellagic acid의 분석법 설정과 분석법에 대한 검증(validation)을 실시하였다.그 결과 ellagic acid의 검량선은 R2=0.9999으로 좋은 선형성을 보였으며, 검출한계 0.6µg/mL, 정량한계는 1.9µg/mL였다. 회수율을 측정한 결과에서는 89.0-100.0%로 나왔고, 상대표준편차(RSD)는 0.05-0.14%를 보였으며, 일내와일간 분석에서 RSD는 각각 0.28-1.96%와 0.69-2.49%로나왔다. 따라서 본 연구를 통해 복분자 추출물의 지표물질인 ellagic acid의 분석법은 적합한 시험법임이 검증되었다.

This study investigated the changes in the nutraceutical lipid components of brown rice oil after germination. Four different high-yielding cultivars (Dasan1, Segyejinmi, Hanareum1 and Hanareum2) of Korean brown rice were selected and brown rice oil was extracted from each cultivar before and after germination. Free fatty acid, squalene, policosanols, and isomers of phytosterol were analyzed using GC, and isomers of tocols (tocopherol and tocotrienol) and γ-oryzanol were quantified using HPLC from both brown rice oil (BRO) and germinated brown rice oil (GBRO). The contents of phytosterol isomers, campesterol, stigmasterol, and β-sitosterol were increased by 8.3%, 31.6%, 3.3% in GBRO, respectively. Furthermore, the squalene content showed the highest increase of up to 2.4 fold in GBRO compared to BRO. In addition, linoleic and linolenic acid composition increased whereas oleic and palmitic acid decreased in the GRBO. However, the contents of tocols (tocopherol and tocotrienol) in GBRO were lower than those in BRO, and there was no significant difference in policosanol and γ-oryzanol between GBRO and BRO. These results suggest that GBRO has the potential as a healthy and functional source due to its lipid profile on improved lipid metabolism.

This study compared the antioxidant activity and functional constituents of the peel, flesh,and placenta of oriental melons according to the harvest time. The sample oriental melons were harvested during the major harvest time (from June to August) and divided into peel, flesh, and placenta. To examine the antioxidant activity and functional constituents, the sample melons were extracted using EtOH. As a result, the functional constituents and antioxidant activity of the oriental melons were found to change according to the harvest time. The total phenol and total flavonoid contents were highest in the samples harvested in June, and lowest in the samples harvested in July. Meanwhile, the antioxidant activities were estimated using an ABTS and FRAP assay, where the samples harvested in June also showed the strongest the antioxidant potential, while the samples harvested in July showed significantly lower antioxidant activities. This means that the climate influences the synthesis of secondary metabolites. The above data also suggests that oriental melons harvested in June contain more functional phytochemicals, making them more beneficial for human health.